Soybean aphids exploit abscisic acid signaling to suppress jasmonate defense responses

Abstract Summary Soybean aphids (Aphis glycines) can induce susceptibility on soybean (Glycine max) during colonization. However, the mechanism for this process is not known. Based on previous transcriptome analyses, we hypothesized that aphids block effective jasmonate (JA) defenses by inducing an antagonistic abscisic acid (ABA) signal. To test this hypothesis, we used a combination of gene expression analyses, measurements of hormone levels, and aphid bioassays on plants with reduced expression of ABA-related genes. Aphid feeding attenuated JA responses in soybean plants and facilitated the growth of a chewing herbivore. Aphid-treated plants had increased levels of cis-JA but not biologically active JA-isoleucine, and aphid feeding induced expression of genes associated with JA-Ile catabolism. In parallel, aphid-feeding induced higher levels of ABA. ABA treatment and knockdown lines impaired in ABA biosynthesis (aba2-RNAi) or signaling (scof-1-RNAi), showed that ABA suppressed wound-induced JA responses. Aphid populations were significantly reduced on ABA-deficient plants and aphid-regulated attenuation of JA signaling was abolished in these lines. Remarkably, plants defective in ABA signaling had increased JA signaling in the absence of stressors. Our results indicate that, in soybean, the ABA pathway is necessary to control basal levels of JA and soybean aphids exploit this ABA-JA antagonism to suppress plant defenses. Competing Interest Statement The authors have declared no competing interest.