A genetically encoded GRAB sensor for measuring serotonin dynamicsin vivo

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Abstract

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating a variety of processes in the brain. To directly visualize the dynamics of 5-HT, we developed a genetically encoded G PC R - A ctivation- B ased 5-HT (GRAB 5-HT ) sensor with high sensitivity, selectivity, and spatiotemporal resolution. GRAB 5-HT , detected 5-HT release in multiple physiological and pathological conditions in both flies and mice, and thus provides new insights into the dynamics and mechanisms of 5-HT signaling.

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europepmc
last seen: 2026-05-19T01:45:01.086888+00:00