Cadmium bioaccumulation and oxidative-stress-induced DNA alterations in the freshwater cladoceran Moina macrocopa (Straus 1820) following consecutive short-term exposure assessments

Cadmium bioaccumulation and oxidative-stress-induced DNA alterations in the freshwater cladoceran Moina macrocopa (Straus 1820) following consecutive short-term exposure assessments | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Cadmium bioaccumulation and oxidative-stress-induced DNA alterations in the freshwater cladoceran Moina macrocopa (Straus 1820) following consecutive short-term exposure assessments Thilomi Samarakoon, Takeshi Fujino, Masayori Hagimori, Rie Saito This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1333898/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Moina macrocopa is a toxicologically less explored, yet widely distributed freshwater cladoceran found throughout the world. The present study is aimed at determining the cadmium (Cd) bioaccumulation and Cd-induced molecular and biochemical biomarkers, including DNA damage and antioxidant enzyme responses (i.e., catalase (CAT) and glutathione S-transferase (GST) activities), in M. macrocopa neonates. Cd bioaccumulation was determined using a Cd-sensitive fluorescence probe after acute exposure to Cd. The results indicated a concentration-dependent Cd bioaccumulation, especially in the gut region, where it was statistically significant in neonates exposed to ≥5 Cd μg/L. DNA damage was determined by the randomly amplified polymorphic DNA (RAPD) technique after a short-term exposure to sublethal Cd concentrations. Significant DNA alterations were observed in individuals exposed to ≥10 Cd μg/L. Short-term exposure to sublethal Cd concentrations did not change CAT levels. However, low Cd concentrations (10 μg/L) significantly induced GST levels, and higher concentrations (50 μg/L) significantly inhibited the GST levels. Taken together, the results of the present study indicated that bioaccumulation of Cd in M. macrocopa causes oxidative stress even at sublethal concentrations, which may be one of the underlying causes of the observed DNA damage. Furthermore, the findings of the present study highlight the importance of utilizing molecular and biochemical biomarkers for toxicity assessment in cladocerans owing to their high sensitivity and rapid responses even under short-term exposure conditions with low concentrations. Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Full Text Tables Tables are only available as a download in the Supplemental Files section. Supplementary Files SamarakoonTTables.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1333898","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":82229956,"identity":"cff08255-8d87-448c-9bb0-5b6bbc7bef72","order_by":0,"name":"Thilomi 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Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-1333898/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1333898/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Moina macrocopa is a toxicologically less explored, yet widely distributed freshwater cladoceran found throughout the world. The present study is aimed at determining the cadmium (Cd) bioaccumulation and Cd-induced molecular and biochemical biomarkers, including DNA damage and antioxidant enzyme responses (i.e., catalase (CAT) and glutathione S-transferase (GST) activities), in M. macrocopa neonates. Cd bioaccumulation was determined using a Cd-sensitive fluorescence probe after acute exposure to Cd. The results indicated a concentration-dependent Cd bioaccumulation, especially in the gut region, where it was statistically significant in neonates exposed to ≥5 Cd μg/L. DNA damage was determined by the randomly amplified polymorphic DNA (RAPD) technique after a short-term exposure to sublethal Cd concentrations. Significant DNA alterations were observed in individuals exposed to ≥10 Cd μg/L. Short-term exposure to sublethal Cd concentrations did not change CAT levels. However, low Cd concentrations (10 μg/L) significantly induced GST levels, and higher concentrations (50 μg/L) significantly inhibited the GST levels. Taken together, the results of the present study indicated that bioaccumulation of Cd in M. macrocopa causes oxidative stress even at sublethal concentrations, which may be one of the underlying causes of the observed DNA damage. 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