Amelioration of Oxidative Stress by Trans-anethole via Modulating Phase I and Phase II Enzymes Against Hepatic Damage Induced by Ccl4 in Male Wistar Rats

Abstract The current study was designed to assess the in vivo hepatoprotective properties of trans-Anethole and it is a principal aromatic component of star anise possessed several therapeutic properties. Results showed that CCl4 treatment elevated the levels of different serum markers like serum glutamate oxaloacetate transaminase (SGOT) by 4.74 fold, serum glutamate pyruvate transaminase (SGPT) by 3.47 fold, aspartate alkaline phosphatase (ALP) by 3.55 fold, direct bilirubin by 3.48 fold and total bilirubin by 2.38 fold in contrast with control. It was observed that decreased levels of various liver antioxidant enzymes viz. CAT, GR, and GSH were significantly ameliorated by the pre-administration of rats with different doses (40, 80, and 160 mg kg-1 bw) of trans-Anethole. Furthermore, pre-treatment of trans-Anethole reduced phase I enzymes level, whereas elevated level of phase II detoxifying enzymes. Furthermore, histopathological examination showed that the treatment with trans-Anethole was potent in defending the liver from CCl4 toxic injury and restored normal hepatic architecture. Also, trans-Anethole regulated p53 and Cyclin D1 expressions in liver tissue relative to group II treated with CCl4. Collectively, the findings of the study showed a strong efficiency of trans-Anethole in ameliorating the effects caused by CCl4 through modulation of antioxidants and xenobiotic-metabolizing enzymes.