Cell viability and extracellular polymeric substances are indispensable for Pb mineralization by Sinorhizobium sp. C10

Cell viability and extracellular polymeric substances are indispensable for Pb mineralization by Sinorhizobium sp. C10 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Cell viability and extracellular polymeric substances are indispensable for Pb mineralization by Sinorhizobium sp. C10 Ying Ge This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9165433/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Lead (Pb) pollution is a pressing global environmental problem due to its threat to ecosystem health. Rhizobia have garnered significant interest since they demonstrate potential to clean up Pb contaminated soils. However, the processes by which rhizobia mediate Pb biomineralization are not well understood. In this study, sp. (C10) isolated from a soil near a mining site, was used to explore the mechanisms of Pb biomineralization facilitated by this strain, with a particular focus placed on the roles of cell viability and extracellular polymeric substances (EPS). It was found that the strain could sustain growth and metabolic functions under Pb stress. The Pb removal rate reached 99.8% after 26 h of with 50 and 150 mg/L Pb treatments. The alkaline phosphatase from C10 cells effectively decomposed organophosphorus compounds in the culture and released soluble phosphate (P), forming stable chloropyromorphite (Pb5(PO4)3Cl). In contrast, heat-inactivated C10 lost the function of secreting alkaline phosphatase and could not form Pb mineral on the cell surface. Moreover, after stripping EPS, the signal intensities of Pb and P were significantly reduced, as evidenced in the SEM-EDS analysis. Overall, these findings confirmed that cell viability and EPS are indispensable in Pb biomineralization induced by C10. General Microbiology biomineralization cell viability extracellular polymeric substances lead Full Text Additional Declarations The authors declare no competing interests. Supplementary Files SupplementaryInformation.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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Rhizobia have garnered significant interest since they demonstrate potential to clean up Pb contaminated soils. However, the processes by which rhizobia mediate Pb biomineralization are not well understood. In this study, sp. (C10) isolated from a soil near a mining site, was used to explore the mechanisms of Pb biomineralization facilitated by this strain, with a particular focus placed on the roles of cell viability and extracellular polymeric substances (EPS). It was found that the strain could sustain growth and metabolic functions under Pb stress. The Pb removal rate reached 99.8% after 26 h of with 50 and 150 mg/L Pb treatments. The alkaline phosphatase from C10 cells effectively decomposed organophosphorus compounds in the culture and released soluble phosphate (P), forming stable chloropyromorphite (Pb5(PO4)3Cl). In contrast, heat-inactivated C10 lost the function of secreting alkaline phosphatase and could not form Pb mineral on the cell surface. 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