Olsenella lakotia SW165 sp. nov., an acetate producing obligate anaerobe with a GC rich genome

ABSTRACT A Gram-positive and obligately anaerobic bacterium was isolated from cecal content of feral chickens in Brookings, South Dakota, USA. The microorganism grew at 37-45° C and pH 6-7.5. This strain produced acetic acid as the primary metabolic end product. Major fatty acids were C 12:0 , C 14:0 , C 14:0 DMA and summed feature 1 (C 13:1 at 12-13 and C 14:0 aldehyde). Phylogenetic analyses based on 16S rRNA gene sequence suggested that strain SW165 belongs to the family Atopobiaceae with the closest relatives being Olsenella profusa DSM 13989 T (96.33% similarity), Olsenella umbonate DSM 26220 T (96.18%) and Olsenella uli DSM 7084 T (96.03%). Genome sequencing revealed a genome size of 2.43 Mbp with a G+C content of 67.59 mol%, which is the highest G+C content among members of the genus Olsenella . Phylogenetic and phenotypic comparison indicated that strain SW165 represents a novel species of the genus Olsenella , for which the name Olsenella lakotia sp. nov. is proposed. The type strain is SW165 (=DSM 107283 T ).