Experimental Studies of Therapeutic Effect of Endostatin Gene Transferred into Endometriosis Rat Model

Objective:To observe the curative effect by transferring endostatin gene into endometriosis rat model.Methods:To establish endometriosis model in Lewis rats by deploying autotransplantation,choosing the sixty-four successful rat models as empirical study objects,divided them randomly into three groups:Injected 2μl lipofectamine-endo-pBud compounds to I group,2μl lipofec-tamine-pBud compounds to II group and 2μl PBS to III group.Taked the ectopic focus tissues to measure the opposing expression of en-dostatin gene by real-time fluorescent quantitative PCR,measure the opposing expression of endostatin protein and expression of ES-HA fusion protein by Western-blot.Detect the level of endostatin and VEGF in serum by ELISA.Detected the endostatin,MMP-2 and MVD in ectopic focus by immunohistochemical assay,and measured the volume of the ectopia focus by vernier cursor.Results:The opposing expressions of endostatin gene were difference between I group and the two control groups has statistical significance(P0.05);ES-HA fusion protein can be detected in the ectopic focus in I group;The opposing expression of endostatin protein is much higher in the ectopic focus of I group than in the two control groups(P0.01).The level of ES is much higher in I group than in the two control groups(P0.01);The level of VEGF is much lower in I group than in the two control groups(P0.01);There was an negative correlation between ES and VEGF in serum(r=-0.805).The expression of ES is much higher in I group than in the two control groups(P0.01);The expres-sion of MMP-2 is much lower in I group than in the two control groups(P0.01);and there was an negative correlation between the ex-pression of ES and MMP-2 in ectopia focus(r=-0.700).The MVD is much lower in I group than in the two control groups(P0.01);The volume of the ectopia focus is smaller in I group than in the two control groups(P0.01) two weeks after transferring.Conclusions:ES gene and protein can expressed in endometriosis rats successfully by injecting lipofectinmine-endo-pBud compounds to the ectopic focus directly,and it has definite curative effect in endometriosis rat model.