Layer-specific speckle tracking analysis of left ventricular systolic function and synchrony in maintenance hemodialysis patients

Layer-specific speckle tracking analysis of left ventricular systolic function and synchrony in maintenance hemodialysis patients | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research article Layer-specific speckle tracking analysis of left ventricular systolic function and synchrony in maintenance hemodialysis patients Chang Liu, Zining Yan, Li Fan, Jun Huang, Dan Shen, Xiangting Song This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.2.14616/v3 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 08 Jan, 2020 Read the published version in BMC Cardiovascular Disorders → Version 3 posted 4 You are reading this latest preprint version Show more versions Abstract Background: This study investigated the value of layer-specific strain analysis by twodimensional speckle tracking echocardiography (2D-STE) for evaluating left ventricular (LV) systolic function and synchrony in maintenance hemodialysis (MHD) patients. Methods: A total of 34 MHD patients and 35 healthy controls were enrolled in this study. Dynamic images were collected at the LV apical long-axis, the four- and twochamber, and the LV short-axis views at the basal, middle, apical segments. The layerspecific speckle tracking (LST) was used to analyze the longitudinal strain (LS) and circumferential strain (CS) of LV sub-endocardium, mid-myocardium, subepicardium, global longitudinal strain (GLS), global circumferential strain (GCS), the LV 17 segment time to peak LS (TTP), the peak strain dispersion (PSD). The differences in these parameters were compared between control and MHD, and the correlation between PSD and each LS parameter was examined. The receiver operator characteristic curve was used to evaluate the efficacy of three myocardial layer LS and CS in the assessment of LV systolic dysfunction in MHD. Results: MHD had comparable left ventricular ejection fraction (LVEF), but significantly smaller GLS, GCS, and three-layer LS and CS compared to the control. The three layer LS of the basal segment, middle segment, and apex segment was significantly reduced in the MHD compared to the normal, while the three myocardial layer CS of the basal segment, middle segment, and apex segment was significantly reduced in the MHD compared to the normal, except for the sub-endocardium of middle and apex segment. MHD had significantly higher TTP of LV 17 segments and PSD compared to controls, and had delayed peak time in most segments. In addition, PSD of MHD was positively correlated with subendocardial and mid-myocardial LS and GLS, but not with subepicardial LS. The area under the curves (AUCs) of sub-endocardial, midmyocardial, and sub-epicardial LS in MHD were 0.894, 0.852, and 0.870, respectively; the AUCs of sub-epicardial, midmyocardial, and sub-endocardial CS were 0.852, 0.837, and 0.669, respectively. Conclusion: LST may detect the early changes of all three-layer LS and CS and PSD in MHD, and is a valuable tool to diagnose LV systolic dysfunction in MHD. Cardiac & Cardiovascular Systems Cardiothoracic Surgery Echocardiography maintenance hemodialysis ventricular function synchronicity layer-specific strain Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Background End stage renal disease (ESRD) is the final manifestation of chronic renal insufficiency. Hemodialysis, which removes blood metabolites, reduces sodium and water retention, and maintains electrolytes and acid-base balance through solute exchange, is the mainstay treatment for ESRD patients who cannot undergo kidney transplantation (1). Long-term hemodialysis (i.e. maintenance hemodialysis, MHD) has been proven to be effective in reducing clinical symptoms and improving the quality of life of ESRD patients. However, despite the advances in hemodialysis technology, the mortality and morbidity of ESRD patients on MHD remains high and the quality of life of these patients is poor (2). Cardiovascular disease is a major complication of ESRD that is caused by metabolic and hemodynamic changes due to ESRD and is associated with high mortality (3-4), accounting for approximately 50% of hemodialysis patient deaths (5). Left ventricular (LV) dysfunction is a critical indicator of cardiac dysfunction and an early clinical manifestation of LV hypertrophy. Thus, LV dysfunction holds important prognostic value for mortality of ESRD patients on MHD (6-7). There is a significant need to employ effective screening methods to identify early changes in cardiac function in ESRD patients on MHD. Two-dimensional speckle tracking echocardiography (2D-STE), which can non-invasively, semi-automatically, and quantitatively analyze ventricular myocardial strain function, has been widely used in the clinic to evaluate cardiac function (8-9). Compared with traditional echocardiography, 2D-STE technology is faster, more accurate, and angle-independent; it can assess myocardial function through longitudinal, circumferential, radial, and torsional motions (10), and thus can examine the global and local left ventricular function with high sensitivity. The layer-specific speckle tracking (LST) technology, derived from 2D-STE, can analyze strain parameters of LV function in three muscular layers – the sub-endocardium, mid-myocardium, and sub-epicardium (11). Therefore, LST allows early detection of subtle ventricular systolic dysfunction (11-13). Previous studies used the LST to assess global longitudinal, circumferential, and radial myocardial strains of dialysis patients (14-16), but few studies have been performed to measure longitudinal and circumferential strains of the LV three-layer myocardium. In addition, LV systolic synchrony is a sensitive parameter that may reflect early cardiac dysfunction. A normal heart has a well-synchronized LV systolic function, and dys-synchrony is observed in stress, such as in response to pharmacological stimulation (17). However, using LST to evaluate LV systolic synchrony in MHD patients has been rarely reported. In the present study, we used LST to evaluate the longitudinal and circumferential strains of the three-layer LV muscles in MHD patients and to study LV systolic synchrony, with the aim of evaluating the value of LST in detecting cardiac dysfunction at an early stage of MHD. Methods Ethics statement This study protocol was approved by the Ethics Committee of Changzhou Second People's Hospital affiliated to Nanjing Medical University, and all participants signed consent. Participant selection A total of 34 ESRD patients on MHD who were admitted to the Changzhou Second People's Hospital affiliated with Nanjing Medical University between September 2018 and June 2019 and 35 sex- and age-matched healthy individuals (the control group) were registered in this study. Patients who had all of the following were included in this study: 1) kidney disease as the primary disease; 2) LV ejection fraction (LVEF) ≥ 50%; 3) all MHD patients were treated with hemodialysis through forearm arteriovenous anastomosis; 4) hemodialysis was performed three times per week, 4 hours each time; 5) hemodialysis lasted 10 - 36 months; and 6) each patient was weighed before and after hemodialysis, and the post-dialysis weight was equal to the ideal dry weight of each patient (clinically determined), and the difference in weight before and after dialysis was equal to the total volume of hemodialysis removed. All patients underwent image acquisition and measurement within 30 minutes after hemodialysis. Patients who had one of the following diseases were excluded from this study: congenital heart disease, valvular heart disease, coronary artery disease, cardiomyopathy and other heart diseases, arrhythmia, and a history of pulmonary hypertension. Control subjects had normal physical examinations, electrocardiograms, x-ray examinations, and echocardiography results, and did not have any of the following diseases: hypertension, diabetes, heart disease, or abnormal liver and kidney function. Data collection The demographic and clinical data of all participants were obtained from interviews and the hospital database. Conventional 2D Doppler echocardiography Thirty-four MHD patients and 35 control subjects underwent conventional 2D Doppler echocardiography (Vivid E9, GE). The M5S probe was used at a frequency of 2 - 4.5 MHz, and M-mode was used to measure the left ventricular internal diameter at end-diastole (LVIDD), left ventricular internal diameter at end-systole (LVIDS), interventricular septal thickness at end-diastole (IVST), left ventricular posterior wall thickness at end-diastole (LVPWT), left atrial diameter (LAD), and LVEF. Each parameter was measured three times and the average was used to determine the left ventricular mass index (LVMI) using the following formula: LVMI = left ventricular mass / body surface area; left ventricular mass (LVM) = 0.8 x 1.04 x [(LVIDD + LVPWT + IVST) 3 -LVIDD 3 ] + 0.6; body surface area (BSA) = 0.0061 x height + 0.0128 x body weight - 0.1529. Subjects were situated in the left lateral position, connected to the electrocardiogram, and requested to hold their breath to ensure image quality. The instrument was adjusted to clearly display the endocardium, with the image frame rate of 60 - 90 frames/s. The following images were collected during three consecutive cardiac cycles: LV apical long-axis, two- and four-chamber, and LV short-axis views of the basal, middle, and apical segments. All images were stored in the hard drive. Data analysis of LV systolic function Dynamic images of the basal, middle, and apical segments obtained at the LV short-axis view, and dynamic images of the long-axis, two- and four- chamber views obtained at the LV apical view were imported into the 2D speckle tracking analysis software (2D-Strain, EchoPac. PC version 201, GE Healthcare, Horten, Norway) for analysis. Briefly, the LV endocardium in the apical long-axis chamber view was first delineated, followed by the automatic depiction of the region of interest (ROI), including the LV sub-endocardial, mid-myocardium, and sub-epicardial. Then, the curves of the endocardium and epicardium were manually adjusted to match the LV wall. The system automatically selected the aortic valve closing time point according to the ECG, and automatically generated the strain curves and values of each segment. Using a similar method, the apical four- and two-chamber heart images were analyzed to obtain the sub-endocardial, mid-myocardial, and sub-epicardial longitudinal strain (LS) of LV 17 segments and their corresponding curves, as well as the bull's eye diagram and to calculate the LV three-layer LS. The LV global longitudinal strain (GLS) is equivalent to the mean LS of the three layers of myocardium. The system also automatically generated LV 17 segment time to peak LS (TTP) and peak strain dispersion (PSD). Three dynamic images of the LV short axis were traced clockwise from the anterior septum, and the software automatically generated ROI including sub-endocardial, mid-myocardial, and sub-epicardial myocardium. If automatic tracking did not yield satisfactory outcomes, manual adjustment was then performed. The system automatically generated the myocardial circumferential strain (CS) of 17 segments and corresponding curves, and calculated the LV three-layer myocardial CS, the CS of the basal, middle, and apical segments. The LV global circumferential strain (GCS) is equivalent to the mean CS of the three layers of myocardium. Evaluation of intra-observer and inter-observer variability Intra-observer and inter-observer variability were examined in this study. We randomly selected 20 subjects from control and MHD groups for this evaluation. The intra-observer differences were compared between the two observations made by the same observer at 1 week. The inter-differences between observers were compared between two independent observers who were blinded to the grouping. The intra-observer and inter-observer variabilities were evaluated using the intra-class correlation coefficients (ICCs) Statistical analysis All statistical analyses were performed using SPSS 22.0 software (IBM SPSS, Statistics, Chicago, IL, USA). Measurement data are expressed as mean ± standard deviation (SD), and significance was set at the level of P < 0.05. The Kolmogorov-Smirnov's test was used to evaluate data normality. The independent sample T test was used to compare normally distributed data, while the non-parametric Mann-Whitney test was used to compare non-normal data. Pearson correlation analysis was used to examine the correlation between normally distributed variables, while Spearman correlation analysis was used for non-normal variables. The LS and CS values of the myocardium in each layer of the control subjects were defined as normal, and the LS and CS values of the myocardium in each layer of MHD patients were defined as abnormal. Sub-endocardial, mid-myocardial, and sub-epicardial LS and CS in MHD patients were analyzed using the receiver operating characteristic curve (ROC). The Yoden index was used to calculate the critical point of each strain value, specificity, and sensitivity. The criteria for ICCs were: “excellent” if ³ 0.80, “good” if 0.61 - 0.79, “moderate” if 0.41 - 0.60, and “poor” ICC £ 0.40. Results Comparison of demographic and clinical characteristics between control and MHD groups We first compared demographic and clinical data between control and MHD groups. As shown in Table 1, MHD patients had significantly higher SBP, DBP, and creatinine (P 0.05). The primary cause of ESRD was glomerulonephritis (55.8%), followed by diabetic nephropathy (17.6%), hypertensive nephrosclerosis (14.7%), and polycystic kidney (11.8%) (Table 1). Comparison of traditio nal echocardiography parameters between control and MHD groups We next compared traditional echocardiography parameters between control and MHD groups. As shown in Table 2, LVEF was comparable between the groups (P > 0.05), but MHD patients had significantly higher LVIDD, LVIDS, IVST, LVPWT, LAD, LVMI (P < 0.01) compared to the control subjects. These observations suggest that MHD patients had impaired cardiac function. Comparison of layer-specific strain parameters between control and MHD groups In both groups, LS and CS of the LV three-layer myocardium were consistent with the following pattern: sub-endocardial > mid-myocardium > sub-epicardial. Compared with the control group, the MHD group had significantly lower LV GLS, GCS, and three-layer myocardial LS and CS (P < 0.01) (Table 3). MHD patients also had significantly lower LS of the LV three-layer at the basal, middle, and apical segments (P < 0.01) (Table 4, Fig. 1). In addition, with the exception of the sub-endocardium at the apical and middle segments, MHD patients had significantly lower CS of other LV layers compared to the control group (P < 0.05 or P < 0.01) (Table 5, Fig. 2). Comparison of LV synchronous parameters between control and MHD groups In the control group, the color of the TTP bull's eye was uniformly green, while the color of the bull's eye in the MHD group was disordered, showing a mix of yellow and red, indicating that LV contraction was poorly synchronized (Fig. 3). The TTP of the LV 17 segments of MHD patients was significantly higher compared to the control group, and the peak time delay was observed in the majority of these segments (Table 6). In addition, MHD patients had significantly higher PSD compared to the control group (P < 0.01, Table 7). Taken together, these findings suggest that MHD patients had poorly synchronized LV systolic contraction, a sign of cardiac dysfunction. Correlation between PSD and GLS with three-layer LV myocardial LS We next examined the correlation between PSD and GLS with three-layer LV myocardial LS. As shown in Table 8 and Figure 4, PSD was positively correlated with sub-endocardial and mid-myocardial LS and GLS (sub-endocardial, r = 0.467, P = 0.005; mid-myocardial, r = 0.513, P = 0.002; GLS, r = 0.463, P = 0.006). There was no significant correlation between PSD and sub-epicardial LS (P = 0.179). ROC curve analysis of the accuracy of three-layer LV myocardial LS and CS in MHD patients The AUC was analyzed to obtain the efficacy of three-layer LV myocardial LS and CS in the diagnosis of LV systolic dysfunction in MHD patients. The AUC values of sub-endocardial, mid-myocardial, and sub-epicardial LS myocardium in MHD patients were approximately 0.894, 0.852, and 0.870, respectively, and the cutoff values were approximately -21.15%, -18.33%, and -17.08%, respectively. The sensitivity of sub-endocardial LS (85.3%) was higher than that of sub-epicardial (82.4%) and mid-myocardial LS (73.5%). The specificity of LS of the sub-endocardial LS (82.9%) was also higher than that of the mid-myocardial (80.0%) and sub-epicardial LS (77.1%). The AUC values of sub-epicardial, mid-myocardial, and sub-endocardial CS in MHD patients were approximately 0.852, 0.837, and 0.669, respectively, and the cutoff values were -11.65%, -17.86%, and -24.33%, respectively. The sensitivity of sub-epicardial CS (82.4%) was higher than that of mid-myocardial (73.5%) and sub-endocardial CS (50.0%). However, the specificity of mid-myocardial CS (82.9%) was higher than that of sub-endocardial (80.0%) and sub-epicardial CS (74.3%) (Table 9, Fig. 5). Collectively, our findings indicate that sub-endocardial LS is an appropriate indicator of early stage cardiac dysfunction. Intra-observer and inter-observer variability We examined the intra-observer and inter-observer variability in our analysis. Twenty participants were randomly selected from the control and MHD groups. All observers were blinded to the subject type and measured the reproducibility of the three-layer LV myocardial LS, CS, GLS, GCS, and PSD. As shown in Table 10, our results suggest that our study generated reliable and consistent observations. Discussion Although MHD is the primary treatment for ESRD patients who are not candidates for kidney replacement, the mortality rate of MHD patients remains high and cardiovascular complications, such as heart failure and coronary heart disease, are the main factors associated with the high mortality of MHD patients (18). Therefore, early detection of cardiac dysfunction in MHD patients has important clinical applications. In the present study, we used LST to evaluate the LS and CS of different LV myocardial layers of control and MHD patients and found that, despite the comparable LVEF between these two groups, MHD patients had altered LS and CS at different myocardial layers, higher TTP of 17 LV segments, and higher PSD compared to control subjects. Our findings suggest that LST holds value for early identifying LV dysfunction of MHD patients. A previous study showed that hemodialysis did not significantly improve LV remodeling and systolic dysfunction in patients (19). Consistent with that report, we first employed traditional echocardiography to assess the cardiac function of control subjects and MHD patients. We found that controls and MHD patients had a relatively comparable LVEF; however, MHD patients had significantly increased cardiac functional indexes, including LAD, LVIDD, LVIDS, IVSD, LVPWD, and LVMI. These findings suggest that ESRD patients exhibit LV remodeling with potential LV systolic dysfunction even after MHD treatment. Indeed, a previous study showed that ESRD patients on MHD had extensive pre- and post-loading factors such as sodium and water retention, anemia, malnutrition, valvular insufficiency, and hypertension (20). The presence of a long-term arteriovenous fistula increased the pre-cardiac load in MHD patients (21). To overcome increases in pre- and post-load, the LV develops physiological cardiac hypertrophy at an early stage to maintain normal cardiac output in MHD patients (22-23). Early studies have shown that the overall LS obtained by 2D-STE has become an objective and sensitive indicator for quantitative analysis of small changes in LV systolic function (24), which can be used to detect changes in regional myocardial blood supply earlier than LVEF (25). LST based on 2D-STE technology is a new modality for evaluating wall motion. Animal experiments showed that LST can better assess the degree of myocardial infarction-induced damage at an early stage (26). Previous studies showed that the mid- and epi- myocardia of LV are sensitive to post-load changes (27-29), and that the endocardial myocardium is sensitive to changes in volumetric load (27,30), indicating that different LV myocardial layers exhibit different responses to myocardial insults. Thus, measuring layer-specific strain could be helpful to accurately assess the subtle changes in LV function during the progression of cardiac diseases. The present study used the layer-specific strain by 2D speckle tracking to appraise cardiac strain parameters of MHD patients, and revealed that MHD patients had significantly decreased myocardial LS of all layers compared to the control group. The impaired myocardial LS of all layers could be attributed to endothelial dysfunction and vascular injury (15), and to the observation that ESRD patients receiving MHD commonly present with anemia, secondary hyperparathyroidism, and arteriovenous fistula, all of which increase volume load and result in LV remodeling, hypertrophy, and fibrosis (31). These pathological changes aggravate the progression of atherosclerosis and further heighten LV wall stress and stiffness (32), eventually leading to decreased endocardial LS (33) . As the disease progresses and dialysis time increases, myocardial fibrosis and LV hypertrophy is exacerbated, leading to a decrease in myocardial strain of each layer. The cardiac myocardium can be divided into three layers: endocardial, middle, and epicardial. Approximately 75% of the endocardial and epicardial layers consist of longitudinal myocardium, while 25% of the middle layer consists of ring-shaped myocardium (34). During myocardial contraction, the middle annular myocardium produces motion in the short axis direction. Compared with the control group, we found that MHD patients had comparable CS at the apical and the middle endocardial segments, indicating that LS was first impaired in MHD patients, while CS was only partially impaired at the early stage of cardiac impairment. Moreover, impaired CS was mainly observed in the middle and epicardial myocardium, which may be related to the fact that the middle myocardium has more ring-shaped myocardium. Since hemodialysis removes excess fluid from the body, the heart consequently increases LV contraction to maintain normal cardiac output and blood pressure (35). However, MHD patients often have compromised cardiac function, and present with fluctuating blood pressure after hemodialysis. Since the mid- and epi-myocardium is sensitive to afterload (27-29), a large range of blood pressure fluctuation may underlie reduced CS in the mid- and epi-cardial myocardium of MHD patients. LV TTP is the time from the R-wave of the electrocardiogram to the longitudinal peak strain of the LV 17 segments, and PSD measures the dispersion of the TTP of the LV 17 segments. Both TTP and PSD can be used to evaluate LV myocardial synchrony (36). Compared with the control group, MHD patients had significantly delayed TTP and increased PSD, indicating decreased LV myocardial synchrony in MHD patients. This decreased LV synchrony could be attributed to increased sodium retention and high blood pressure. Indeed, some studies have shown that hypertension causes LV remodeling and alters myocardial electrophysiological properties, such as increased cardiomyocyte autorhythmicity and potential instability, myocardial electrophysiological conduction block, and myocardial excitation-contraction coupling dysregulation, all of which may lead to impaired LV systolic synchrony (37-38). Due to excessive volume overload, elevated peripheral blood pressure, and lack of ATP, LV contraction time is extended as a compensatory mechanism to insure adequate cardiac output (39). Indeed, we found that PSD was positively correlated with GLS, which also indicates that the decrease in LV contraction in MHD patients is accompanied by a decrease in contractile synchrony. We also found that PSD was positively correlated with sub-endocardial and mid-myocardial GLS, but not with sub-epicardial GLS. This finding was probably due to the distribution of His bundle, left bundle branch, and Purkinje fiber mainly in sub-endocardium and mid-myocardium, thus rendering these myocardia more susceptible to the above factors (40). Previously, Shi et al (41) studied LV strain of young and middle-aged patients undergoing peritoneal dialysis, and found that sub-endocardial GLS was more sensitive to blood perfusion than the epicardium. Sun et al (42) used LST technology to study LV function in ESRD patients, and reported that the curvature radius of the annular myocardial fiber was smaller than the curvature radius of the longitudinal myocardial fiber. Moreover, they found that the tension of the circumferential myocardial fiber was lower when deformed. Thus, they concluded that the LS of the myocardial fiber was more sensitive than CS. Leng et al (43) also found that the decrease in LS occurred earlier than CS in response to external insults. In the present study, we analyzed the efficacy of three-layer strain parameters for predicting LV systolic function in MHD patients, and found that the AUC of the sub-endocardial LS was the largest with a cut-off value of -21.15%, indicating that the sub-endocardial LS was the best index to evaluate LV systolic function. The AUC of the mid- and sub-epicardial CS was greater than that of the sub-endocardial CS. Also, the AUC of the sub-endocardial LS was greater than that of the mid- and epi-cardial CS. Thus, our findings are in line with the previous reports. Limitations The limitations of this study should be acknowledged. First, our study was a single-centered study with a small sample size. Thus, we were not able to further divide MHD patients into subgroups. Second, the MHD patients in this study routinely took antihypertensive drugs, including calcium channel blockers and angiotensin converting enzyme inhibitors, and we did not rule out the possibility that the LV functional changes detected in MHD patients were already ameliorated by these treatments to some degree. Third, our study focused on LV longitudinal, circumferential strain, and synchrony, and did not include LV radial strain and torsion. Fourth, all MHD patients were assessed for coronary artery disease only by symptoms, electrocardiogram, myocardial enzyme profile, troponin, and echocardiography; they were not evaluated using angiography. Thus, our study did not rule out the possibility that some of these patients had subclinical coronary artery disease. Finally, the relationship between left ventricular load changes and left ventricular strain values revealed in MHD patients in this study needs to be further corroborated in the future. Conclusions We report here that, although MHD patients had relatively normal LVEF, they had significantly reduced LS and CS of sub-endocardial, mid-myocardial, and sub-epicardial layers as detected by the LST. MHD patients also had significantly increased PSD, suggesting impaired LV systolic synchrony. Therefore, LST can detect changes of three-layer strain, LV systolic function, and dys-synchrony in MHD patients with high sensitivity. Our findings provide direct evidence that LST may be used clinically to detect cardiac dysfunction at an early stage in MHD patients to guide cardiac treatment. Abbreviations 2D-STE: Two-dimensional speckle tracking echocardiography; MHD: Maintenance hemodialysis; LV: Left ventricular; LST: Layer-specific speckle tracking; LS: Longitudinal strain; CS: Circumferential strain; GLS: Global longitudinal strain; GCS: Global circumferential strain; TTP: Time to peak longitudinal strain; PSD: Peak strain dispersion; ESRD: End stage renal disease; LVEF: Left ventricular ejection fraction; LVIDD: Left ventricular internal diameter at end-diastole; LVIDS: Left ventricular internal diameter at end-systole; IVST: Interventricular septal thickness at end-diastole; LVPWT: Left ventricular posterior wall thickness at end-diastole; LAD: Left atrial diameter; LVMI: Left ventricular mass index Declarations Ethics approval and consent to participate This study complied with the Declaration of Helsinki and was reviewed and approved by the Human Subjects Committee of Changzhou No.2 People’s Hospital approved this study. Written informed consent was obtained from the participant enrolled in this study. Consent for publication Not applicable Availability of data and materials The datasets generated and/or analyzed during the current study are available in the Open Science Framework repository, https://mfr.osf.io/render?url=https://osf.io/bngwh/?action=download%26mode=render Competing interests The authors declare that they have no competing interests. Funding None Authors’ Contributions LC and YZN designed the study, collected, and analyzed echocardiography measurements. LC wrote the manuscript. YZN and FL revised the manuscript. FL and LC collected the clinical data. SD and HJ check the validity of data. LC and SXT performed the statistical analysis. All authors have read and approved the final manuscript. Acknowledgements Not applicable References Shibiru T , Gudina EK , Habte B , Derbew A , Agonafer T . 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A novel multi-layer approach of measuring myocardial strain and torsion by 2D speckle tracking imaging in normal subjects and patients with heart diseases. Int J Cardiol. 2011;147(1):32-7. Duan F, Xie M, Wang X, Li Y, He L, Jiang L, et al. Preliminary clinical study of left ventricular myocardial strain in patients with non-ischemic dilated cardiomyopathy by three-dimensional speckle tracking imaging. Cardiovasc Ultrasound. 2012;10:8. Imbalzano E, Zito C, Carerj S, Oreto G, Mandraffino G, Cusma-Piccione M, et al: Left ventricular function in hypertension: new insight by speckle tracking echocardiography. Echocardiography. 2011;28(6):649-57. Nie Y, Zhang Z, Zou J, Liang Y, Cao X, Liu Z, et al. Hemodialysis-induced regional left ventricular systolic dysfunction. Hemodial Int. 2016;20(4):564-72. Widmer RJ , Lerman A . Endothelial dysfunction and cardiovascular disease. Glob Cardiol Sci Pract . 2014;2014(3): 291–308 Montgomery DE , Puthumana JJ , Fox JM , Ogunyankin KO . 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Correlation between systolic deformation and dyssynchrony indices and the grade of left ventricular hypertrophy in hypertensive patients with a preserved systolic ejection fraction undergoing coronary angiography, based on tissue Doppler imaging. J Ultrasound Med. 2014;33(1):119-28. Ma Y, Zhang B, Zhang Y, Dong Y, Zhang R. Ultrasonic image analysis of longitudinal strain in uremic patients with preserved left ventricular ejection fraction. Biomed Eng Online. 2018;17(1):112. Oh IY, Cha MJ, Lee TH, Seo JW, Oh S. Unsolved Questions on the Anatomy of the Ventricular Conduction System. Korean Circ J. 2018;48(12):1081-96. Shi F, Feng S, Zhu J, Wu Y, Chen J. Left Ventricular Strain and Dyssynchrony in Young and Middle-Aged Peritoneal Dialysis Patients and Healthy Controls: A Case-Matched Study. Cardiorenal Med. 2018;8(4):271-84. Sun M, Dong Y, Wang Y, Li G, Huang D. Assessment of the left ventricular function in patients with uremia using layer-specific 2-dimensional speckle tracking echocardiography. Medicine (Baltimore). 2019;98(9):e14656. Leng Z, Li R, Li Y, Wang L, Wang Y, Yang Y. Myocardial layer-specific analysis in patients with heterozygous familial hypercholesterolemia using speckle tracking echocardiography. Echocardiography. 2017;34(3):390-96. Tables Table 1. Comparison of demographic and clinical characteristics between control and MHD groups MHD (n=34) Control (n=35) P-value Age (years) 52±9 49±10 0.327 Male gender (%) 52.9 48.6 HD time (months) 23(10-36) Heart rate (beats/min) 76.79±9.63 72.34±10.50 0.071 BMI 22.18±2.11 21.29±2.14 0.086 Remove volume (kg) Dry weight (kg) SBP (mmHg) 1.82±0.93 52.07±8.95 144.65±17.00 121.40±6.49 <0.001 DBP (mmHg) 88.82±11.17 75.91±6.29 <0.001 Creatinine (umol/L) 855.41±191.36 55.86±9.92 <0.001 Cause of ESRD Glomerulonephritis (cases) 19 (55.8%) Diabetic Nephropathy (cases) 6 (17.6%) Hypertensive nephrosclerosis (cases) 5 (14.7%) Polycystic kidney (cases) 4 (11.8%) Data are expressed as mean ± SD. Bold number: P < 0.05 HD: hemodialysis; BMI: body mass index; BP: systolic blood pressure; DBP: diastolic blood pressure; Creatinine: serum creatinine; ESRD: end-stage renal disease. T able 2. Comparison of traditional echocardiography parameters between control and MHD groups MHD Normal P-value LVIDD (mm) 52.56±6.13 46.06±3.07 <0.001 LVIDS (mm) 36.65±5.55 31.11±2.84 <0.001 IVST (mm) 11.56±1.62 8.86±1.38 <0.001 LVPWT (mm) 11.24±1.50 8.14±1.38 <0.001 LAD (mm) 42.38±5.29 34.86±3.12 <0.001 LVEF (%) 58.24±5.16 60.11±4.00 0.095 LVMI (g/m 2 ) 56.59±9.11 31.60±3.82 <0.001 Data are expressed as mean ± SD. Bold number: P < 0.05 LVIDD: left ventricular internal diameter at end-diastole; LVIDS: left ventricular internal diameter at end-systole; IVST: interventricular septal thickness at end-diastole; LVPWT: left ventricular posterior wall thickness at end-diastole; LAD: left atrial diameter; LVEF: left ventricular ejection fraction; LVMI: left ventricular mass index. T able 3. Comparison of global and layer-specific LS and CS between control and MHD groups Varible Longitudinal strain (%) Circumferential strain(%) Sub-endocardial Mid-myocardial Sub-epicardial Global Sub-endocardial Mid-myocardial Subepicardial Global MHD -17.04±4.09 -14.76±4.11 -13.50±2.99 -15.07±3.46 -24.70±4.68 -15.33±2.44 -9.20±1.67 -16.41±2.59 Normal -23.41±3.58 -20.18±3.25 -18.07±2.75 -20.55±3.16 -27.40±3.51 -18.94±2.74 -12.36±2.52 -19.57±2.75 P-value <0.001 <0.001 <0.001 <0.001 0.008 <0.001 <0.001 <0.001 Data are expressed as mean ± SD. Bold number: P < 0.05 Table 4. Comparison of horizontal layer-specific longitudinal strain between control and MHD groups Segmental LV wall Longitudinal strain (%) Endocardial Middle Epicardial MHD Normal P-value MHD Normal P-value MHD Normal P-value basal segment -12.47±4.23 -19.57±2.92 <0.001 -12.15±4.32 -18.66±2.58 <0.001 -12.15±3.53 -18.06±2.75 <0.001 middle segment -16.56±4.42 -22.40±3.42 <0.001 -14.82±3.93 -19.83±3.28 <0.001 -14.38±3.14 -18.37±2.61 <0.001 apex segment -22.09±5.37 -28.26±5.26 <0.001 -17.32±5.26 -22.06±4.54 <0.001 -13.65±4.12 -17.77±3.43 <0.001 Data are expressed as mean ± SD. Bold number: P < 0.05 Table 5. Comparison of horizontal layer-spe cific circumferential strain parameters between control and MHD patients Segmental LV wall Circumferential strain(%) Sub-endocardial Mid-myocardial sub-epicardial MHD Normal P-value MHD Normal P-value MHD Normal P-value basal segment -23.88±5.99 -27.46±5.37 0.011 -15.15±3.60 -19.09±4.18 <0.001 -9.47±2.62 -13.11±3.64 <0.001 middle segment -23.79±5.40 -26.00±3.80 0.053 -14.44±3.52 -17.97±3.26 <0.001 -8.56±3.15 -12.43±3.17 <0.001 apex segment -26.41±7.23 -28.74±5.25 0.131 -16.41±3.49 -19.77±4.03 <0.001 -9.56±3.41 -11.54±3.39 0.018 Data are expressed as mean ± SD. Bold number: P < 0.05 Table 6. Comparison of time to peak longitudinal strain of LV 17 segments between control and MHD groups variable TTP (ms) MHD Normal P-value bas ANT-SEPT 415±93 375±55 0.032 bas ANT 424±85 375±53 0.006 bas LAT 451±73 383±57 <0.001 bas POST 405±54 379±51 0.045 bas INF 394±56 367±33 0.017 bas SEPT 389±71 376±52 0.669 mid ANT-SEPT 375±56 363±42 0.499 mid ANT 403±80 360±39 0.006 mid LAT 431±67 366±38 <0.001 mid POST 401±51 366±41 0.002 mid INF 381±48 360±30 0.028 mid SEPT 368±52 360±33 0.094 ap ANT 389±60 365±33 0.044 ap LAT 385±59 369±33 0.151 ap INF 397±61 358±28 0.001 ap SEPT 370±46 362±33 0.372 ap AP 383±46 364±28 0.047 Data are expressed as mean ± SD. Bold number: P < 0.05 Bas: basal segment; mid: middle segment; ap: apex segment; ANT-SEPT: anterior septum wall; ANT: anterior wall; LAT: lateral wall; POST: posterior wall; INF: inferior wall; SEPT: septum wall; TTP: time to peak longitudinal strain. Table 7. Comparison of LV PSD between control and MHD groups Group n PSD MHD 34 57.26±18.18 Normal 35 29.97±8.31 P-value <0.001 Data are expressed as mean ± SD. PSD: peak strain dispersion. Bold number: P < 0.05 Table 8. Correlation between PSD and LV three-layer myocardial LS and GLS Longitudinal strain (%) Endocardial Middle Epicardial Global r-value 0.467 0.513 0.236 0.463 p-value 0.005 0.002 0.179 0.006 Bold number: P < 0.05 Table 9. RO C curve analysis of accuracy of LV three-layer myocardial LS and CS in MHD patients Parameters AUC 95%CI Cut-off Value (%) Sensitivity (%) Specificity(%) Youden index LS sub-endocardial 0.894 0.796-0.955 -21.15 85.3 82.9 0.6521 LS mid-myocardial 0.852 0.746-0.926 -18.33 73.5 80.0 0.5353 LS sub-epicardial 0.870 0.767-0.939 -17.08 82.4 77.1 0.6244 CS sub-endocardial 0.669 0.545-0.778 -24.33 50.0 80.0 0.3000 CS mid-myocardial 0.837 0.728-0.915 -17.86 73.5 82.9 0.5639 CS sub-epicardial 0.852 0.746-0.926 -11.65 82.4 74.3 0.5664 AUC: area under the curve; 95% CI: 95% confidence interval; LS: longitudinal strain; CS: circumferential strain. Table 10. Evaluation of intra-observer and inter-observer variability Intra-observer Inter-observer ICC 95%CI ICC 95%CI LS sub-endocardial 0.90 0.76-0.96 0.87 0.69-0.94 LS mid-myocardial 0.89 0.76-0.96 0.79 0.55-0.91 LS sub-epicardial 0.87 0.70-0.95 0.88 0.72-0.95 GLS 0.89 0.75-0.96 0.87 0.71-0.95 CS sub-endocardial 0.89 0.74-0.95 0.87 0.71-0.93 CS mid-myocardial 0.77 0.51-0.90 0.82 0.60-0.92 CS sub-epicardial 0.86 0.69-0.94 0.80 0.57-0.92 GCS 0.84 0.63-0.93 0.83 0.62-0.93 PSD 0.96 0.90-0.98 0.95 0.88-0.98 ICC: intra-class correlation coefficients; 95% CI ： 95% confidence interval; LS: longitudinal strain; GLS: global longitudinal strain; CS: circumferential strain; GCS: global circumferential strain; PSD: peak strain dispersion. Cite Share Download PDF Status: Published Journal Publication published 08 Jan, 2020 Read the published version in BMC Cardiovascular Disorders → Version 3 posted Editorial decision: Accept 30 Dec, 2019 Editor assigned by journal 27 Dec, 2019 Submission checks completed at journal 26 Dec, 2019 Editor invited by journal 26 Dec, 2019 You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5293","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":272045,"identity":"1908f781-63d2-42c2-b9f7-932bfa0e1a5b","order_by":1,"name":"Chang 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19:33:35","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":126827,"visible":true,"origin":"","legend":"Bull’s eyes of the peak LS of the sub-endocardial, mid-myocardial, and sub-epicardial layers in MHD patients and normal subjects.","description":"","filename":"fig1.jpg","url":"https://assets-eu.researchsquare.com/files/70c1ee66-e21f-4997-b68b-3bbc82f4bf20/v3/fig 1.jpg"},{"id":314012,"identity":"86f3c8f2-76db-4b40-a4bd-6c8383c39dcf","added_by":"auto","created_at":"2019-12-31 19:33:35","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":180638,"visible":true,"origin":"","legend":"Peak CS of the sub-endocardial, mid-myocardial, and sub-epicardial layers of LV in MHD patients and normal subjects.","description":"","filename":"fig2.jpg","url":"https://assets-eu.researchsquare.com/files/70c1ee66-e21f-4997-b68b-3bbc82f4bf20/v3/fig 2.jpg"},{"id":314013,"identity":"c9969344-5246-4e53-9f3d-5f0b7c2ffb32","added_by":"auto","created_at":"2019-12-31 19:33:35","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":173838,"visible":true,"origin":"","legend":"Bull’s eyes of the time to peak LS for the multiple LV segments and their PSD between MHD patients and normal subjects.","description":"","filename":"fig3.jpg","url":"https://assets-eu.researchsquare.com/files/70c1ee66-e21f-4997-b68b-3bbc82f4bf20/v3/fig 3.jpg"},{"id":314014,"identity":"d0f44a07-48a9-4728-9c54-313db456791f","added_by":"auto","created_at":"2019-12-31 19:33:36","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":40689,"visible":true,"origin":"","legend":"Correlation between PSD and peak LS of the sub-endocardial (a), mid-myocardial (b) and sub-epicardial layers (c), and the GLS (d) in MHD patients.","description":"","filename":"fig4.jpg","url":"https://assets-eu.researchsquare.com/files/70c1ee66-e21f-4997-b68b-3bbc82f4bf20/v3/fig 4.jpg"},{"id":314015,"identity":"24bf9d55-fff9-4920-81f4-4fa99a7eac95","added_by":"auto","created_at":"2019-12-31 19:33:36","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":32241,"visible":true,"origin":"","legend":"ROC curve analysis for evaluating the sensitivity and specificity of the peak LS (a) and peak CS (b) of different myocardial layers in identifying LV dysfunction in MHD patients.","description":"","filename":"fig5.jpg","url":"https://assets-eu.researchsquare.com/files/70c1ee66-e21f-4997-b68b-3bbc82f4bf20/v3/fig 5.jpg"},{"id":13483867,"identity":"eb3d1a6d-8523-45c5-aa34-1d5c0d0412dd","added_by":"auto","created_at":"2021-09-16 21:55:28","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1063207,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5293/v3/2f8d5e62-80c3-4f13-8cdc-64de9e2e2020.pdf"}],"financialInterests":"","formattedTitle":"Layer-specific speckle tracking analysis of left ventricular systolic function and synchrony in maintenance hemodialysis patients","fulltext":[{"header":"Background","content":"\u003cp\u003eEnd stage renal disease (ESRD) is the final manifestation of chronic renal insufficiency. Hemodialysis, which removes blood metabolites, reduces sodium and water retention, and maintains electrolytes and acid-base balance through solute exchange, is the mainstay treatment for ESRD patients who cannot undergo kidney transplantation (1). Long-term hemodialysis (i.e. maintenance hemodialysis, MHD) has been proven to be effective in reducing clinical symptoms and improving the quality of life of ESRD patients. However, despite the advances in hemodialysis technology, the mortality and morbidity of ESRD patients on MHD remains high and the quality of life of these patients is poor (2). Cardiovascular disease is a major complication of ESRD that is caused by metabolic and hemodynamic changes due to ESRD and is associated with high mortality (3-4), accounting for approximately 50% of hemodialysis patient deaths (5). Left ventricular (LV) dysfunction is a critical indicator of cardiac dysfunction and an early clinical manifestation of LV hypertrophy. Thus, LV dysfunction holds important prognostic value for mortality of ESRD patients on MHD (6-7). There is a significant need to employ effective screening methods to identify early changes in cardiac function in ESRD patients on MHD.\u003c/p\u003e\n\u003cp\u003eTwo-dimensional speckle tracking echocardiography (2D-STE), which can non-invasively, semi-automatically, and quantitatively analyze ventricular myocardial strain function, has been widely used in the clinic to evaluate cardiac function (8-9). Compared with traditional echocardiography, 2D-STE technology is faster, more accurate, and angle-independent; it can assess myocardial function through longitudinal, circumferential, radial, and torsional motions (10), and thus can examine the global and local left ventricular function with high sensitivity. The layer-specific speckle tracking (LST) technology, derived from 2D-STE, can analyze strain parameters of LV function in three muscular layers \u0026ndash; the sub-endocardium, mid-myocardium, and sub-epicardium (11). Therefore, LST allows early detection of subtle ventricular systolic dysfunction (11-13). Previous studies used the LST to assess global longitudinal, circumferential, and radial myocardial strains of dialysis patients (14-16), but few studies have been performed to measure longitudinal and circumferential strains of the LV three-layer myocardium. In addition, LV systolic synchrony is a sensitive parameter that may reflect early cardiac dysfunction. A normal heart has a well-synchronized LV systolic function, and dys-synchrony is observed in stress, such as in response to pharmacological stimulation (17). However, using LST to evaluate LV systolic synchrony in MHD patients has been rarely reported.\u003c/p\u003e\n\u003cp\u003eIn the present study, we used LST to evaluate the longitudinal and circumferential strains of the three-layer LV muscles in MHD patients and to study LV systolic synchrony, with the aim of evaluating the value of LST in detecting cardiac dysfunction at an early stage of MHD.\u003c/p\u003e"},{"header":"Methods","content":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eEthics statement\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study protocol was approved by the Ethics Committee of Changzhou Second People's Hospital affiliated to Nanjing Medical University, and all participants signed consent.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eParticipant selection\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eA total of 34 ESRD patients on MHD who were admitted to the Changzhou Second People's Hospital affiliated with Nanjing Medical University between September 2018 and June 2019 and 35 sex- and age-matched healthy individuals (the control group) were registered in this study. Patients who had all of the following were included in this study: 1) kidney disease as the primary disease; 2) LV ejection fraction (LVEF) \u0026ge; 50%; 3) all MHD patients were treated with hemodialysis through forearm arteriovenous anastomosis; 4) hemodialysis was performed three times per week, 4 hours each time; 5) hemodialysis lasted 10 - 36 months; and 6) each patient was weighed before and after hemodialysis, and the post-dialysis weight was equal to the ideal dry weight of each patient (clinically determined), and the difference in weight before and after dialysis was equal to the total volume of hemodialysis removed. All patients underwent image acquisition and measurement within 30 minutes after hemodialysis. Patients who had one of the following diseases were excluded from this study: congenital heart disease, valvular heart disease, coronary artery disease, cardiomyopathy and other heart diseases, arrhythmia, and a history of pulmonary hypertension. Control subjects had normal physical examinations, electrocardiograms, x-ray examinations, and echocardiography results, and did not have any of the following diseases: hypertension, diabetes, heart disease, or abnormal liver and kidney function.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eData collection\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe demographic and clinical data of all participants were obtained from interviews and the hospital database.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eConventional 2D Doppler echocardiography\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThirty-four MHD patients and 35 control subjects underwent conventional 2D Doppler echocardiography (Vivid E9, GE). The M5S probe was used at a frequency of 2 - 4.5 MHz, and M-mode was used to measure the left ventricular internal diameter at end-diastole (LVIDD), left ventricular internal diameter at end-systole (LVIDS), interventricular septal thickness at end-diastole (IVST), left ventricular posterior wall thickness at end-diastole (LVPWT), left atrial diameter (LAD), and LVEF. Each parameter was measured three times and the average was used to determine the left ventricular mass index (LVMI) using the following formula: LVMI = left ventricular mass / body surface area; left ventricular mass (LVM) = 0.8 x 1.04 x [(LVIDD + LVPWT + IVST)\u003csup\u003e3\u003c/sup\u003e-LVIDD\u003csup\u003e3\u003c/sup\u003e] + 0.6; body surface area (BSA) = 0.0061 x height + 0.0128 x body weight - 0.1529.\u003c/p\u003e\n\u003cp\u003eSubjects were situated in the left lateral position, connected to the electrocardiogram, and requested to hold their breath to ensure image quality. The instrument was adjusted to clearly display the endocardium, with the image frame rate of 60 - 90 frames/s. The following images were collected during three consecutive cardiac cycles: LV apical long-axis, two- and four-chamber, and LV short-axis views of the basal, middle, and apical segments. All images were stored in the hard drive.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eData analysis of LV systolic function\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eDynamic images of the basal, middle, and apical segments obtained at the LV short-axis view, and dynamic images of the long-axis, two- and four- chamber views obtained at the LV apical view were imported into the 2D speckle tracking analysis software (2D-Strain, EchoPac. PC version 201, GE Healthcare, Horten, Norway) for analysis. Briefly, the LV endocardium in the apical long-axis chamber view was first delineated, followed by the automatic depiction of the region of interest (ROI), including the LV sub-endocardial, mid-myocardium, and sub-epicardial. Then, the curves of the endocardium and epicardium were manually adjusted to match the LV wall. The system automatically selected the aortic valve closing time point according to the ECG, and automatically generated the strain curves and values of each segment.\u003c/p\u003e\n\u003cp\u003eUsing a similar method, the apical four- and two-chamber heart images were analyzed to obtain the sub-endocardial, mid-myocardial, and sub-epicardial longitudinal strain (LS) of LV 17 segments and their corresponding curves, as well as the bull's eye diagram and to calculate the LV three-layer LS. The LV global longitudinal strain (GLS) is equivalent to the mean LS of the three layers of myocardium. The system also automatically generated LV 17 segment time to peak LS (TTP) and peak strain dispersion (PSD). Three dynamic images of the LV short axis were traced clockwise from the anterior septum, and the software automatically generated ROI including sub-endocardial, mid-myocardial, and sub-epicardial myocardium. If automatic tracking did not yield satisfactory outcomes, manual adjustment was then performed. The system automatically generated the myocardial circumferential strain (CS) of 17 segments and corresponding curves, and calculated the LV three-layer myocardial CS, the CS of the basal, middle, and apical segments. The LV global circumferential strain (GCS) is equivalent to the mean CS of the three layers of myocardium.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eEvaluation of intra-observer and inter-observer variability \u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIntra-observer and inter-observer variability were examined in this study. We randomly selected 20 subjects from control and MHD groups for this evaluation. The intra-observer differences were compared between the two observations made by the same observer at 1 week. The inter-differences between observers were compared between two independent observers who were blinded to the grouping. The intra-observer and inter-observer variabilities were evaluated using the intra-class correlation coefficients (ICCs)\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eStatistical analysis\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll statistical analyses were performed using SPSS 22.0 software (IBM SPSS, Statistics, Chicago, IL, USA). Measurement data are expressed as mean \u0026plusmn; standard deviation (SD), and significance was set at the level of P \u0026lt; 0.05. The Kolmogorov-Smirnov's test was used to evaluate data normality. The independent sample T test was used to compare normally distributed data, while the non-parametric Mann-Whitney test was used to compare non-normal data. Pearson correlation analysis was used to examine the correlation between normally distributed variables, while Spearman correlation analysis was used for non-normal variables. The LS and CS values of the myocardium in each layer of the control subjects were defined as normal, and the LS and CS values of the myocardium in each layer of MHD patients were defined as abnormal. Sub-endocardial, mid-myocardial, and sub-epicardial LS and CS in MHD patients were analyzed using the receiver operating characteristic curve (ROC). The Yoden index was used to calculate the critical point of each strain value, specificity, and sensitivity. The criteria for ICCs were: \u0026ldquo;excellent\u0026rdquo; if \u0026sup3; 0.80, \u0026ldquo;good\u0026rdquo; if 0.61 - 0.79, \u0026ldquo;moderate\u0026rdquo; if 0.41 - 0.60, and \u0026ldquo;poor\u0026rdquo; ICC \u0026pound; 0.40.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eComparison of demographic and clinical characteristics between control and MHD groups\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe first compared demographic and clinical data between control and MHD groups. As shown in Table 1, MHD patients had significantly higher SBP, DBP, and creatinine (P \u0026lt; 0.05) compared to the control group, but there were no significant differences with regard to age, gender, heart rate, and body mass index (BMI) between these two groups (P \u0026gt; 0.05). The primary cause of ESRD was glomerulonephritis (55.8%), followed by diabetic nephropathy (17.6%), hypertensive nephrosclerosis (14.7%), and polycystic kidney (11.8%) (Table 1).\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eComparison of traditio\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003enal \u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003eechocardiography\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cem\u003e parameters between control and MHD groups\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe next compared traditional echocardiography parameters between control and MHD groups. As shown in Table 2, LVEF was comparable between the groups (P \u0026gt; 0.05), but MHD patients had significantly higher LVIDD, LVIDS, IVST, LVPWT, LAD, LVMI (P \u0026lt; 0.01) compared to the control subjects. These observations suggest that MHD patients had impaired cardiac function.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eComparison of layer-specific strain parameters between control and MHD groups\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn both groups, LS and CS of the LV three-layer myocardium were consistent with the following pattern: sub-endocardial \u0026gt; mid-myocardium \u0026gt; sub-epicardial. Compared with the control group, the MHD group had significantly lower LV GLS, GCS, and three-layer myocardial LS and CS (P \u0026lt; 0.01) (Table 3). MHD patients also had significantly lower LS of the LV three-layer at the basal, middle, and apical segments (P \u0026lt; 0.01) (Table 4, Fig. 1). In addition, with the exception of the sub-endocardium at the apical and middle segments, MHD patients had significantly lower CS of other LV layers compared to the control group (P \u0026lt; 0.05 or P \u0026lt; 0.01) (Table 5, Fig. 2).\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eComparison of LV synchronous parameters between control and MHD groups \u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn the control group, the color of the TTP bull's eye was uniformly green, while the color of the bull's eye in the MHD group was disordered, showing a mix of yellow and red, indicating that LV contraction was poorly synchronized (Fig. 3). The TTP of the LV 17 segments of MHD patients was significantly higher compared to the control group, and the peak time delay was observed in the majority of these segments (Table 6). In addition, MHD patients had significantly higher PSD compared to the control group (P \u0026lt; 0.01, Table 7). Taken together, these findings suggest that MHD patients had poorly synchronized LV systolic contraction, a sign of cardiac dysfunction.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eCorrelation between PSD and GLS with three-layer LV myocardial LS\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe next examined the correlation between PSD and GLS with three-layer LV myocardial LS. As shown in Table 8 and Figure 4, PSD was positively correlated with sub-endocardial and mid-myocardial LS and GLS (sub-endocardial, r = 0.467, P = 0.005; mid-myocardial, r = 0.513, P = 0.002; GLS, r = 0.463, P = 0.006). There was no significant correlation between PSD and sub-epicardial LS (P = 0.179).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eROC curve analysis of the accuracy of three-layer LV myocardial LS and CS in MHD patients \u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe AUC was analyzed to obtain the efficacy of three-layer LV myocardial LS and CS in the diagnosis of LV systolic dysfunction in MHD patients. The AUC values of sub-endocardial, mid-myocardial, and sub-epicardial LS myocardium in MHD patients were approximately 0.894, 0.852, and 0.870, respectively, and the cutoff values were approximately -21.15%, -18.33%, and -17.08%, respectively. The sensitivity of sub-endocardial LS (85.3%) was higher than that of sub-epicardial (82.4%) and mid-myocardial LS (73.5%). The specificity of LS of the sub-endocardial LS (82.9%) was also higher than that of the mid-myocardial (80.0%) and sub-epicardial LS (77.1%). The AUC values of sub-epicardial, mid-myocardial, and sub-endocardial CS in MHD patients were approximately 0.852, 0.837, and 0.669, respectively, and the cutoff values were -11.65%, -17.86%, and -24.33%, respectively. The sensitivity of sub-epicardial CS (82.4%) was higher than that of mid-myocardial (73.5%) and sub-endocardial CS (50.0%). However, the specificity of mid-myocardial CS (82.9%) was higher than that of sub-endocardial (80.0%) and sub-epicardial CS (74.3%) (Table 9, Fig. 5). Collectively, our findings indicate that sub-endocardial LS is an appropriate indicator of early stage cardiac dysfunction.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eIntra-observer and inter-observer variability \u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe examined the intra-observer and inter-observer variability in our analysis. Twenty participants were randomly selected from the control and MHD groups. All observers were blinded to the subject type and measured the reproducibility of the three-layer LV myocardial LS, CS, GLS, GCS, and PSD. As shown in Table 10, our results suggest that our study generated reliable and consistent observations.\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eAlthough MHD is the primary treatment for ESRD patients who are not candidates for kidney replacement, the mortality rate of MHD patients remains high and cardiovascular complications, such as heart failure and coronary heart disease, are the main factors associated with the high mortality of MHD patients (18). Therefore, early detection of cardiac dysfunction in MHD patients has important clinical applications. In the present study, we used LST to evaluate the LS and CS of different LV myocardial layers of control and MHD patients and found that, despite the comparable LVEF between these two groups, MHD patients had altered LS and CS at different myocardial layers, higher TTP of 17 LV segments, and higher PSD compared to control subjects. Our findings suggest that LST holds value for early identifying LV dysfunction of MHD patients.\u003c/p\u003e\n\u003cp\u003eA previous study showed that hemodialysis did not significantly improve LV remodeling and systolic dysfunction in patients (19). Consistent with that report, we first employed traditional echocardiography to assess the cardiac function of control subjects and MHD patients. We found that controls and MHD patients had a relatively comparable LVEF; however, MHD patients had significantly increased cardiac functional indexes, including LAD, LVIDD, LVIDS, IVSD, LVPWD, and LVMI. These findings suggest that ESRD patients exhibit LV remodeling with potential LV systolic dysfunction even after MHD treatment. Indeed, a previous study showed that ESRD patients on MHD had extensive pre- and post-loading factors such as sodium and water retention, anemia, malnutrition, valvular insufficiency, and hypertension (20). The presence of a long-term arteriovenous fistula increased the pre-cardiac load in MHD patients (21). To overcome increases in pre- and post-load, the LV develops physiological cardiac hypertrophy at an early stage to maintain normal cardiac output in MHD patients (22-23).\u003c/p\u003e\n\u003cp\u003eEarly studies have shown that the overall LS obtained by 2D-STE has become an objective and sensitive indicator for quantitative analysis of small changes in LV systolic function (24), which can be used to detect changes in regional myocardial blood supply earlier than LVEF (25). LST based on 2D-STE technology is a new modality for evaluating wall motion. Animal experiments showed that LST can better assess the degree of myocardial infarction-induced damage at an early stage (26). Previous studies showed that the mid- and epi- myocardia of LV are sensitive to post-load changes (27-29), and that the endocardial myocardium is sensitive to changes in volumetric load (27,30), indicating that different LV myocardial layers exhibit different responses to myocardial insults. Thus, measuring layer-specific strain could be helpful to accurately assess the subtle changes in LV function during the progression of cardiac diseases. The present study used the layer-specific strain by 2D speckle tracking to appraise cardiac strain parameters of MHD patients, and revealed that MHD patients had significantly decreased myocardial LS of all layers compared to the control group. The impaired myocardial LS of all layers could be attributed to endothelial dysfunction and vascular injury (15), and to the observation that ESRD patients receiving MHD commonly present with anemia, secondary hyperparathyroidism, and arteriovenous fistula, all of which increase volume load and result in LV remodeling, hypertrophy, and fibrosis (31). These pathological changes aggravate the progression of atherosclerosis and further heighten LV wall stress and stiffness (32), eventually leading to decreased endocardial LS \u003cem\u003e(33)\u003c/em\u003e. As the disease progresses and dialysis time increases, myocardial fibrosis and LV hypertrophy is exacerbated, leading to a decrease in myocardial strain of each layer.\u003c/p\u003e\n\u003cp\u003eThe cardiac myocardium can be divided into three layers: endocardial, middle, and epicardial. Approximately 75% of the endocardial and epicardial layers consist of longitudinal myocardium, while 25% of the middle layer consists of ring-shaped myocardium (34). During myocardial contraction, the middle annular myocardium produces motion in the short axis direction. Compared with the control group, we found that MHD patients had comparable CS at the apical and the middle endocardial segments, indicating that LS was first impaired in MHD patients, while CS was only partially impaired at the early stage of cardiac impairment. Moreover, impaired CS was mainly observed in the middle and epicardial myocardium, which may be related to the fact that the middle myocardium has more ring-shaped myocardium. Since hemodialysis removes excess fluid from the body, the heart consequently increases LV contraction to maintain normal cardiac output and blood pressure (35). However, MHD patients often have compromised cardiac function, and present with fluctuating blood pressure after hemodialysis. Since the mid- and epi-myocardium is sensitive to afterload (27-29), a large range of blood pressure fluctuation may underlie reduced CS in the mid- and epi-cardial myocardium of MHD patients.\u003c/p\u003e\n\u003cp\u003eLV TTP is the time from the R-wave of the electrocardiogram to the longitudinal peak strain of the LV 17 segments, and PSD measures the dispersion of the TTP of the LV 17 segments. Both TTP and PSD can be used to evaluate LV myocardial synchrony (36). Compared with the control group, MHD patients had significantly delayed TTP and increased PSD, indicating decreased LV myocardial synchrony in MHD patients. This decreased LV synchrony could be attributed to increased sodium retention and high blood pressure. Indeed, some studies have shown that hypertension causes LV remodeling and alters myocardial electrophysiological properties, such as increased cardiomyocyte autorhythmicity and potential instability, myocardial electrophysiological conduction block, and myocardial excitation-contraction coupling dysregulation, all of which may lead to impaired LV systolic synchrony (37-38). Due to excessive volume overload, elevated peripheral blood pressure, and lack of ATP, LV contraction time is extended as a compensatory mechanism to insure adequate cardiac output (39). Indeed, we found that PSD was positively correlated with GLS, which also indicates that the decrease in LV contraction in MHD patients is accompanied by a decrease in contractile synchrony. We also found that PSD was positively correlated with sub-endocardial and mid-myocardial GLS, but not with sub-epicardial GLS. This finding was probably due to the distribution of His bundle, left bundle branch, and Purkinje fiber mainly in sub-endocardium and mid-myocardium, thus rendering these myocardia more susceptible to the above factors (40).\u003c/p\u003e\n\u003cp\u003ePreviously, Shi et al (41) studied LV strain of young and middle-aged patients undergoing peritoneal dialysis, and found that sub-endocardial GLS was more sensitive to blood perfusion than the epicardium. Sun et al (42) used LST technology to study LV function in ESRD patients, and reported that the curvature radius of the annular myocardial fiber was smaller than the curvature radius of the longitudinal myocardial fiber. Moreover, they found that the tension of the circumferential myocardial fiber was lower when deformed. Thus, they concluded that the LS of the myocardial fiber was more sensitive than CS. Leng et al (43) also found that the decrease in LS occurred earlier than CS in response to external insults. In the present study, we analyzed the efficacy of three-layer strain parameters for predicting LV systolic function in MHD patients, and found that the AUC of the sub-endocardial LS was the largest with a cut-off value of -21.15%, indicating that the sub-endocardial LS was the best index to evaluate LV systolic function. The AUC of the mid- and sub-epicardial CS was greater than that of the sub-endocardial CS. Also, the AUC of the sub-endocardial LS was greater than that of the mid- and epi-cardial CS. Thus, our findings are in line with the previous reports.\u003c/p\u003e"},{"header":"Limitations","content":"\u003cp\u003eThe limitations of this study should be acknowledged. First, our study was a single-centered study with a small sample size. Thus, we were not able to further divide MHD patients into subgroups. Second, the MHD patients in this study routinely took antihypertensive drugs, including calcium channel blockers and angiotensin converting enzyme inhibitors, and we did not rule out the possibility that the LV functional changes detected in MHD patients were already ameliorated by these treatments to some degree. Third, our study focused on LV longitudinal, circumferential strain, and synchrony, and did not include LV radial strain and torsion. Fourth, all MHD patients were assessed for coronary artery disease only by symptoms, electrocardiogram, myocardial enzyme profile, troponin, and echocardiography; they were not evaluated using angiography. Thus, our study did not rule out the possibility that some of these patients had subclinical coronary artery disease. Finally, the relationship between left ventricular load changes and left ventricular strain values revealed in MHD patients in this study needs to be further corroborated in the future.\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eWe report here that, although MHD patients had relatively normal LVEF, they had significantly reduced LS and CS of sub-endocardial, mid-myocardial, and sub-epicardial layers as detected by the LST. MHD patients also had significantly increased PSD, suggesting impaired LV systolic synchrony. Therefore, LST can detect changes of three-layer strain, LV systolic function, and dys-synchrony in MHD patients with high sensitivity. Our findings provide direct evidence that LST may be used clinically to detect cardiac dysfunction at an early stage in MHD patients to guide cardiac treatment. \u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003e2D-STE: Two-dimensional speckle tracking echocardiography; MHD: Maintenance hemodialysis; LV: Left ventricular; LST: Layer-specific speckle tracking; LS: Longitudinal strain; CS: Circumferential strain; GLS: Global longitudinal strain; GCS: Global circumferential strain; TTP: Time to peak longitudinal strain; PSD: Peak strain dispersion; ESRD: End stage renal disease; LVEF: Left ventricular ejection fraction; LVIDD: Left ventricular internal diameter at end-diastole; LVIDS: Left ventricular internal diameter at end-systole; IVST: Interventricular septal thickness at end-diastole; LVPWT: Left ventricular posterior wall thickness at end-diastole; LAD: Left atrial diameter; LVMI: Left ventricular mass index \u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study complied with the Declaration of Helsinki and was reviewed and approved by the Human Subjects Committee of Changzhou No.2 People\u0026rsquo;s Hospital approved this study. Written informed consent was obtained from the participant enrolled in this study.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe datasets generated and/or analyzed during the current study are available in the Open Science Framework repository,\u003c/p\u003e\n\u003cp\u003ehttps://mfr.osf.io/render?url=https://osf.io/bngwh/?action=download%26mode=render\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNone\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; Contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eLC and YZN designed the study, collected, and analyzed echocardiography measurements. LC wrote the manuscript. YZN and FL revised the manuscript. FL and LC collected the clinical data. SD and HJ check the validity of data. LC and SXT performed the statistical analysis. All authors have read and approved the final manuscript.\u0026nbsp;\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003e\u003ca href=\"https://www.ncbi.nlm.nih.gov/pubmed/?term=Shibiru%20T%5BAuthor%5D\u0026amp;cauthor=true\u0026amp;cauthor_uid=23782468\"\u003eShibiru T\u003c/a\u003e, \u003ca href=\"https://www.ncbi.nlm.nih.gov/pubmed/?term=Gudina%20EK%5BAuthor%5D\u0026amp;cauthor=true\u0026amp;cauthor_uid=23782468\"\u003eGudina EK\u003c/a\u003e, \u003ca href=\"https://www.ncbi.nlm.nih.gov/pubmed/?term=Habte%20B%5BAuthor%5D\u0026amp;cauthor=true\u0026amp;cauthor_uid=23782468\"\u003eHabte B\u003c/a\u003e, \u003ca href=\"https://www.ncbi.nlm.nih.gov/pubmed/?term=Derbew%20A%5BAuthor%5D\u0026amp;cauthor=true\u0026amp;cauthor_uid=23782468\"\u003eDerbew A\u003c/a\u003e, \u003ca href=\"https://www.ncbi.nlm.nih.gov/pubmed/?term=Agonafer%20T%5BAuthor%5D\u0026amp;cauthor=true\u0026amp;cauthor_uid=23782468\"\u003eAgonafer T\u003c/a\u003e. 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Echocardiography. 2017;34(3):390-96.\u003c/li\u003e\n\u003c/ol\u003e "},{"header":"Tables","content":"\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;line-height:150%;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eTable 1.\u003c/span\u003e\u003c/strong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:#222222;\"\u003eComparison of demographic and clinical characteristics between control and MHD groups\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eMHD (n=34)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eControl (n=35)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.8pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eAge (years)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.8pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e52±9\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.8pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e49±10\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.8pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.327\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eMale gender (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e52.9\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e48.6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eHD time (months)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e23(10-36)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eHeart rate (beats/min)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e76.79±9.63\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e72.34±10.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.071\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eBMI\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e22.18±2.11\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e21.29±2.14\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.086\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eRemove volume\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;(kg)\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eDry weight\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;(kg)\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eSBP (mmHg)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e1.82±0.93\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e52.07±8.95\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e144.65±17.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e121.40±6.49\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eDBP (mmHg)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e88.82±11.17\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e75.91±6.29\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eCreatinine (umol/L)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e855.41±191.36\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e55.86±9.92\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eCause of ESRD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;text-indent:.5in;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eGlomerulonephritis (cases)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e19 (55.8%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;text-indent:.5in;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eDiabetic Nephropathy (cases)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e6 (17.6%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;margin-left:35.9pt;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eHypertensive nephrosclerosis (cases)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e5 (14.7%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:15.1pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:199.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"46.58493870402802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Polycystic kidney (cases)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.45pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"20.140105078809107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e4 (11.8%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:78.55pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"18.38879159369527%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:63.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:16.55pt;\" width=\"14.8861646234676%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eHD: hemodialysis; BMI: body mass index; BP: systolic blood pressure; DBP: diastolic blood pressure; Creatinine: serum creatinine; ESRD: end-stage renal disease.\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;line-height:150%;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eT\u003cspan style=\"color:black;\"\u003eable 2.\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eComparison of traditional\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;line-height:150%;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eechocardiography\u003cspan style=\"color:black;\"\u003e\u0026nbsp;parameters between control and MHD groups\u003c/span\u003e\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVIDD (mm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e52.56±6.13\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e46.06±3.07\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVIDS (mm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e36.65±5.55\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e31.11±2.84\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eIVST (mm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e11.56±1.62\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e8.86±1.38\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVPWT (mm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e11.24±1.50\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e8.14±1.38\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLAD (mm)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e42.38±5.29\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e34.86±3.12\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVEF (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e58.24±5.16\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e60.11±4.00\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.095\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:92.3pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"29.567307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVMI (g/m\u003csup\u003e2\u003c/sup\u003e)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"23.317307692307693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e56.59±9.11\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.55pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"22.83653846153846%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e31.60±3.82\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.55pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:4.5pt;\" width=\"24.278846153846153%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLVIDD: left ventricular internal diameter at end-diastole; LVIDS: left ventricular internal diameter at end-systole; IVST: interventricular septal thickness at end-diastole; LVPWT: left ventricular posterior wall thickness at end-diastole; LAD: left atrial diameter; LVEF: left ventricular ejection fraction; LVMI: left ventricular mass index.\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eT\u003cspan style=\"color:black;\"\u003eable 3. Comparison of global and layer-specific LS and CS between control and MHD groups\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/strong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width:.75in;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"8.017817371937639%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eVarible\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width:288.8pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"42.87305122494432%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLongitudinal strain (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-top:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"1.78173719376392%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width:318.7pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"47.32739420935412%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCircumferential strain(%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:76.5pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"12.34866828087167%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.25pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"13.922518159806295%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:64.5pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"10.411622276029055%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSub-epicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:61.55pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"9.927360774818402%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"1.937046004842615%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.2pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"11.501210653753027%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.75pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"15.13317191283293%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.25pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"12.469733656174334%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSubepicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"12.34866828087167%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:.75in;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"8.017817371937639%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.04±4.09\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"12.806236080178174%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-14.76±4.11\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:64.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"9.57683741648107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-13.50±2.99\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:61.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"9.131403118040089%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-15.07±3.46\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"1.78173719376392%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.2pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"10.579064587973274%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-24.70±4.68\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"13.919821826280623%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-15.33±2.44\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"11.469933184855234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-9.20±1.67\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:36.6pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-16.41±2.59\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:.75in;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"8.017817371937639%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-23.41±3.58\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"12.806236080178174%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-20.18±3.25\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:64.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"9.57683741648107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.07±2.75\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:61.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"9.131403118040089%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-20.55±3.16\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"1.78173719376392%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.2pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"10.579064587973274%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-27.40±3.51\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"13.919821826280623%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.94±2.74\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"11.469933184855234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-12.36±2.52\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:34.3pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-19.57±2.75\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:.75in;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"8.017817371937639%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:86.25pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"12.806236080178174%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:64.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"9.57683741648107%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:61.55pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"9.131403118040089%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"1.78173719376392%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.2pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"10.579064587973274%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.008\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"13.919821826280623%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.25pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"11.469933184855234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:76.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.9pt;\" width=\"11.35857461024499%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eTable 4. Comparison of horizontal layer-specific longitudinal strain between control and MHD groups\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"3\" style=\"width:56.6pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"8.43644544431946%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSegmental LV wall\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"11\" style=\"width:610.75pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"91.56355455568054%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLongitudinal strain (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"3\" style=\"width:192.05pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"31.411042944785276%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eEndocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"1.9631901840490797%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width:195.5pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"32.02453987730061%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMiddle\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"1.9631901840490797%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width:199.2pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:23.95pt;\" width=\"32.63803680981595%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eEpicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:69.75pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"11.425061425061426%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:1.0in;border-top:solid windowtext 1.0pt;border-left: none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"11.793611793611793%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.3pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"8.230958230958231%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"1.9656019656019657%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.35pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"12.039312039312039%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.65pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"11.793611793611793%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.5pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"8.230958230958231%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"1.9656019656019657%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"13.267813267813267%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.85pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"11.547911547911548%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:47.5pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"7.73955773955774%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:56.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"8.43644544431946%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ebasal segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"10.46119235095613%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-12.47±4.23\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:1.0in;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-19.57±2.92\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"11.023622047244094%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-12.15±4.32\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.66±2.58\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"12.148481439820022%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-12.15±3.53\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"10.573678290213723%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.06±2.75\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:47.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:31.35pt;\" width=\"7.086614173228346%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:56.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"8.43644544431946%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003emiddle segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"10.46119235095613%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-16.56±4.42\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:1.0in;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-22.40±3.42\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.3pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"11.023622047244094%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-14.82±3.93\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-19.83±3.28\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"12.148481439820022%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-14.38±3.14\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"10.573678290213723%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.37±2.61\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:47.5pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:26.6pt;\" width=\"7.086614173228346%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:56.6pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"8.43644544431946%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eapex segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.46119235095613%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-22.09±5.37\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:1.0in;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-28.26±5.26\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.3pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.35pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"11.023622047244094%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.32±5.26\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.798650168728908%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-22.06±4.54\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:50.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.536557930258717%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"1.7997750281214848%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"12.148481439820022%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-13.65±4.12\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.573678290213723%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.77±3.43\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:47.5pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.086614173228346%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eTable 5. Comparison of horizontal layer-spe\u003cspan style=\"color:black;\"\u003ecific circumferential strain parameters between control and MHD patients\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"3\" style=\"width:66.0pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSegmental LV wall\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"11\" style=\"width:602.25pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"90.12345679012346%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCircumferential strain(%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"3\" style=\"width:186.9pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"31.008717310087174%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"1.9925280199252802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width:194.85pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.378580323785805%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"1.9925280199252802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width:196.5pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.62764632627646%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003esub-epicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:72.75pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"12.079701120797012%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.0pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"10.95890410958904%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:48.15pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"7.970112079701121%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"1.9925280199252802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:74.1pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"12.32876712328767%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"11.581569115815691%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"8.468244084682441%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"1.9925280199252802%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"12.079701120797012%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"12.079701120797012%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:22.05pt;\" width=\"8.468244084682441%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:66.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ebasal segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-23.88±5.99\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-27.46±5.37\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:48.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"7.18294051627385%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.011\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:74.1pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"11.11111111111111%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-15.15±3.60\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"10.437710437710438%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-19.09±4.18\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-9.47±2.62\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-13.11±3.64\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:38.85pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:66.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003emiddle segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-23.79±5.40\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-26.00±3.80\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:48.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"7.18294051627385%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.053\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:74.1pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"11.11111111111111%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-14.44±3.52\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"10.437710437710438%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.97±3.26\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-8.56±3.15\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-12.43±3.17\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:42.45pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:66.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eapex segment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-26.41±7.23\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"9.876543209876543%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-28.74±5.25\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:48.15pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.18294051627385%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.131\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:74.1pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"11.11111111111111%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-16.41±3.49\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:69.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.437710437710438%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-19.77±4.03\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"1.7957351290684624%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-9.56±3.41\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:72.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"10.886644219977553%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-11.54±3.39\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:51.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:38.1pt;\" width=\"7.631874298540965%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.018\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cpre style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:13px;font-family:\u0026quot;Courier New\u0026quot;;line-height:18.0pt;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eTable 6. \u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eComparison of time to peak longitudinal strain of LV 17 segments between control and MHD groups\u003c/span\u003e\u003c/strong\u003e\u003c/pre\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width:98.25pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003evariable\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"3\" style=\"width:227.25pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"69.81566820276498%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eTTP (ms)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:71.25pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"31.353135313531354%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"35.31353135313531%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"33.333333333333336%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ebas ANT-SEPT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e415±93\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e375±55\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.032\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ebas ANT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e424±85\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e375±53\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.006\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ebas LAT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp 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style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003emid ANT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e403±80\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp 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width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e381±48\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e360±30\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.028\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003emid SEPT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e368±52\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e360±33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.094\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eap ANT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e389±60\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e365±33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.044\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eap LAT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e385±59\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e369±33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.151\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eap INF\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e397±61\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e358±28\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eap SEPT\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e370±46\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e362±33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.372\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:98.25pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.18433179723502%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eap AP\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:71.25pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.889400921658986%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e383±46\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.25pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.65437788018433%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e364±28\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.75pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.27188940092166%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.047\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eBas: basal segment; mid: middle segment; ap: apex segment; ANT-SEPT: anterior septum wall; ANT: anterior wall; LAT: lateral wall; POST: posterior wall;\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eINF: inferior wall; SEPT: septum wall; TTP: time to peak longitudinal strain.\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eTable 7.\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;background:#F8F9FA;\"\u003eComparison of LV PSD between control and MHD groups\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:126.65pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.31357552581262%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eGroup\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:118.35pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.21032504780115%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003en\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:146.65pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"37.476099426386234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003ePSD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:126.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.31357552581262%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eMHD\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:118.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.21032504780115%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e34\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:146.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"37.476099426386234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e57.26±18.18\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:126.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.31357552581262%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eNormal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:118.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.21032504780115%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e35\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:146.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"37.476099426386234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e29.97±8.31\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:126.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"32.31357552581262%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eP-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:118.35pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"30.21032504780115%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:146.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"37.476099426386234%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026lt;0.001\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eData are expressed as mean ± SD. PSD: peak strain dispersion. Bold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eTable 8.\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;Correlation between PSD and LV three-layer myocardial LS and GLS\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width:57.95pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"13.824057450628366%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"4\" style=\"width:359.8pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"86.17594254937164%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eLongitudinal strain (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:84.35pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"23.38204592901879%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eEndocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.65pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"20.45929018789144%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eMiddle\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:92.6pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"25.678496868475992%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eEpicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:109.2pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"30.48016701461378%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eGlobal\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:57.95pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"13.848920863309353%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003er-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"20.14388489208633%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.467\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"17.62589928057554%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.513\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:92.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"22.12230215827338%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.236\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:109.2pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:16.5pt;\" width=\"26.258992805755394%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.463\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:57.95pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"13.848920863309353%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003ep-value\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.35pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"20.14388489208633%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.005\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"17.62589928057554%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.002\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:92.6pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"22.12230215827338%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.179\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:109.2pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:17.25pt;\" width=\"26.258992805755394%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.006\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eBold number: P \u0026lt; 0.05\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eTable 9.\u003cspan style=\"color:red;\"\u003e\u0026nbsp;\u003c/span\u003eRO\u003cspan style=\"color:black;\"\u003eC curve analysis of accuracy of LV three-layer myocardial LS and CS in MHD patients\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eParameters\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eAUC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e95%CI\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCut-off Value (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSensitivity (%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eSpecificity(%)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eYouden index\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLS sub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.894\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.796-0.955\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-21.15\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e85.3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e82.9\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.6521\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLS mid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.852\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.746-0.926\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-18.33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e73.5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e80.0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.5353\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eLS sub-epicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.870\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.767-0.939\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.08\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e82.4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e77.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.6244\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCS sub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.669\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.545-0.778\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-24.33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e50.0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e80.0\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.3000\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCS mid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.837\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.728-0.915\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-17.86\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e73.5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e82.9\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.5639\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:136.05pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"22.12713936430318%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eCS sub-epicardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:49.05pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"7.946210268948655%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.852\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:80.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.202933985330073%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.746-0.926\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:84.15pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.691931540342297%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e-11.65\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"13.32518337408313%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e82.4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:93.35pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.158924205378973%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e74.3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:89.15pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"14.547677261613693%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e0.5664\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eAUC: area under the curve; 95% CI: 95% confidence interval; LS: longitudinal strain; CS: circumferential strain.\u003c/span\u003e\u003c/p\u003e\u003c/br\u003e\u003c/br\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:left;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003eTable 10. Evaluation of\u003c/span\u003e\u003c/strong\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003cstrong\u003eintra-observer and inter-observer variability\u003c/strong\u003e\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;color:black;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003ctable align=\"left\" border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse:collapse;border:none;margin-left:6.75pt;margin-right:6.75pt;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd rowspan=\"2\" style=\"width:108.4pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"25.985663082437277%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width:142.45pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"34.05017921146953%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eIntra-observer\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-top:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"2.867383512544803%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" style=\"width:155.5pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"37.096774193548384%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eInter-observer\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:66.6pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"21.54963680387409%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eICC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.85pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"24.455205811138015%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e95%CI\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"3.87409200968523%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.85pt;border:none;border-bottom:solid windowtext 1.0pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"23.728813559322035%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eICC\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border-top:solid windowtext 1.0pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"26.39225181598063%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e95%CI\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:108.4pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"25.985663082437277%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eLS sub-endocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.949820788530467%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.90\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"18.100358422939067%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.76-0.96\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"2.867383512544803%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"17.56272401433692%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.87\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"19.53405017921147%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.69-0.94\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:108.4pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"25.985663082437277%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eLS mid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.949820788530467%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.89\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"18.100358422939067%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.76-0.96\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"2.867383512544803%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.85pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"17.56272401433692%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.79\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"19.53405017921147%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.55-0.91\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:108.4pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"25.985663082437277%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eLS sub-epicardial\u003c/span\u003e\u003c/p\u003e\n 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style=\"width:81.65pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"19.53405017921147%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.71-0.93\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:108.4pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"25.985663082437277%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eCS mid-myocardial\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:66.6pt;border:none;padding:0in 5.4pt 0in 5.4pt;\" width=\"15.949820788530467%\"\u003e\n \u003cp 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Roman\u0026quot;,serif;\"\u003e0.96\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:75.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"18.100358422939067%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.90-0.98\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:12.0pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"2.867383512544803%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:73.85pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"17.56272401433692%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.95\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:81.65pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;\" width=\"19.53405017921147%\"\u003e\n \u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:center;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e0.88-0.98\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style=\"margin:0in;margin-bottom:.0001pt;text-align:justify;font-size:14px;font-family:\u0026quot;Calibri\u0026quot;,sans-serif;\"\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003eICC: intra-class correlation coefficients; 95% CI\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:SimSun;\"\u003e：\u003c/span\u003e\u003cspan style=\"font-size:16px;font-family:\u0026quot;Times New Roman\u0026quot;,serif;\"\u003e95% confidence interval; LS: longitudinal strain; GLS: global longitudinal strain; CS: circumferential strain; GCS: global circumferential strain; PSD: peak strain dispersion.\u003c/span\u003e\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Echocardiography, maintenance hemodialysis, ventricular function, synchronicity, layer-specific strain","lastPublishedDoi":"10.21203/rs.2.14616/v3","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.2.14616/v3","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Background: This study investigated the value of layer-specific strain analysis by twodimensional speckle tracking echocardiography (2D-STE) for evaluating left ventricular (LV) systolic function and synchrony in maintenance hemodialysis (MHD) patients. Methods: A total of 34 MHD patients and 35 healthy controls were enrolled in this study. Dynamic images were collected at the LV apical long-axis, the four- and twochamber, and the LV short-axis views at the basal, middle, apical segments. The layerspecific speckle tracking (LST) was used to analyze the longitudinal strain (LS) and circumferential strain (CS) of LV sub-endocardium, mid-myocardium, subepicardium, global longitudinal strain (GLS), global circumferential strain (GCS), the LV 17 segment time to peak LS (TTP), the peak strain dispersion (PSD). The differences in these parameters were compared between control and MHD, and the correlation between PSD and each LS parameter was examined. The receiver operator characteristic curve was used to evaluate the efficacy of three myocardial layer LS and CS in the assessment of LV systolic dysfunction in MHD. Results: MHD had comparable left ventricular ejection fraction (LVEF), but significantly smaller GLS, GCS, and three-layer LS and CS compared to the control. The three layer LS of the basal segment, middle segment, and apex segment was significantly reduced in the MHD compared to the normal, while the three myocardial layer CS of the basal segment, middle segment, and apex segment was significantly reduced in the MHD compared to the normal, except for the sub-endocardium of middle and apex segment. MHD had significantly higher TTP of LV 17 segments and PSD compared to controls, and had delayed peak time in most segments. In addition, PSD of MHD was positively correlated with subendocardial and mid-myocardial LS and GLS, but not with subepicardial LS. The area under the curves (AUCs) of sub-endocardial, midmyocardial, and sub-epicardial LS in MHD were 0.894, 0.852, and 0.870, respectively; the AUCs of sub-epicardial, midmyocardial, and sub-endocardial CS were 0.852, 0.837, and 0.669, respectively. Conclusion: LST may detect the early changes of all three-layer LS and CS and PSD in MHD, and is a valuable tool to diagnose LV systolic dysfunction in MHD.","manuscriptTitle":"Layer-specific speckle tracking analysis of left ventricular systolic function and synchrony in maintenance hemodialysis patients","msid":"","msnumber":"","nonDraftVersions":[{"code":3,"date":"2019-12-31 19:33:35","doi":"10.21203/rs.2.14616/v3","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Accept","date":"2019-12-30T12:00:00+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2019-12-27T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2019-12-26T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2019-12-26T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":2,"date":"2019-11-25 17:13:43","doi":"10.21203/rs.2.14616/v2","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Minor revision","date":"2019-12-18T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2019-12-10T12:00:00+00:00","index":1,"fulltext":"Recommendation: Accept without revision\nForm responses:\n---\n* Are the methods appropriate and well described?: **Yes**\n* Does the work include the necessary controls?: **Yes**\n* Are the conclusions drawn adequately supported by the data shown?: **Yes**\n* Are you able to assess any statistics in the manuscript or would you recommend an additional statistical review?: **I am able to assess the statistics**\n* Quality of written English: **Acceptable**\n* Declaration of competing interests: **none**\n\nComments to Author:\n---\nThe authors have provided appropriate responses to the comments raised by other reviewers. I don't have anything else to add. There are limitations related to the small sample size and the strain measurements according to myocardial layers. These measurements have not been validated and they have limited application in clinical practice currently. However, there is potential and the hypothesis is interesting."},{"type":"editorInvitedReview","content":"","date":"2019-12-09T12:00:00+00:00","index":2,"fulltext":"Recommendation: Reject\nForm responses:\n---\n* Are the methods appropriate and well described?: **Yes**\n* Does the work include the necessary controls?: **Yes**\n* Are the conclusions drawn adequately supported by the data shown?: **No**\n* Are you able to assess any statistics in the manuscript or would you recommend an additional statistical review?: **I am able to assess the statistics**\n* Quality of written English: **Acceptable**\n* Declaration of competing interests: **I declare that I have no competing interests**\n\nComments to Author:\n---\nThe authors showed decreased strain values in the ESRD patients with maintenance hemodialysis (MHD) with layer-specific strains compared with controls. I have several concerns about this study.\n1. The fundamental question is why we should measure layer-specific strain (LST) instead of using standard strains. The authors showed decreased strain values. What is the additional diagnostic value in using LST over the conventional strain?\n2. LV strain values can be affected by preload and afterload (Voigt JU, et al. J Am Coll Cardiol Imag 2019;12:1849-63). In ESRD patients with MHD, preload can be changed from increased venous return as the result of arteriovenous fistulae. Also, increased total body water and toxic materials from decreased urinary excretion and altered cardiac geometry by increased afterload can affect the LV strain values. Thus, patients with MHD may have decreased strain values. However, the authors only showed the decreased strain values in these patients compared with controls. What is the meaning of decreased strain values in these patients? Do they have a higher mortality rate or increased clinical events?\n3. Because of the EchoPAC PC software set mid-myocardial strain value same as the global strain value including 3 layers. Thus, in table 3, the values should be rechecked."},{"type":"reviewerAgreed","content":"","date":"2019-12-08T12:00:00+00:00","index":2,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2019-12-04T12:00:00+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2019-12-04T12:00:00+00:00","index":1,"fulltext":""},{"type":"editorAssigned","content":"","date":"2019-11-25T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2019-11-24T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2019-11-21T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2019-09-19 04:08:17","doi":"10.21203/rs.2.14616/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Minor revision","date":"2019-10-29T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2019-10-02T12:00:00+00:00","index":2,"fulltext":"Form responses:\n---\n* Are the methods appropriate and well described?: **Yes**\n* Does the work include the necessary controls?: **Yes**\n* Are the conclusions drawn adequately supported by the data shown?: **Yes**\n* Are you able to assess any statistics in the manuscript or would you recommend an additional statistical review?: **I recommend additional statistical review**\n* Quality of written English: **Acceptable**\n* Declaration of competing interests: **I declare that I have no competing interests**\n* Does the work include the necessary controls?\nIf not, please specify which controls are required in your comments to the authors.\tYes: **Are the conclusions drawn adequately supported by the data shown?\nIf not, please explain in your comments to the authors.\tYes**\n"},{"type":"editorInvitedReview","content":"","date":"2019-10-01T12:00:00+00:00","index":1,"fulltext":"Form responses:\n---\n* Are the methods appropriate and well described?: **Yes**\n* Does the work include the necessary controls?: **Yes**\n* Are the conclusions drawn adequately supported by the data shown?: **Yes**\n* Are you able to assess any statistics in the manuscript or would you recommend an additional statistical review?: **I am able to assess the statistics**\n* Quality of written English: **Acceptable**\n* Declaration of competing interests: **I declare that I have no competing interests**\n* Does the work include the necessary controls?\nIf not, please specify which controls are required in your comments to the authors.\tYes: **Are the conclusions drawn adequately supported by the data shown?\nIf not, please explain in your comments to the authors.\tYes**\n"},{"type":"reviewerAgreed","content":"","date":"2019-09-19T12:00:00+00:00","index":2,"fulltext":""},{"type":"reviewerAgreed","content":"","date":"2019-09-18T12:00:00+00:00","index":1,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2019-09-17T12:00:00+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2019-09-16T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2019-09-13T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2019-09-12T12:00:00+00:00","index":"","fulltext":""},{"type":"submitted","content":"","date":"2019-08-29T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-cardiovascular-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bcar","sideBox":"Learn more about [BMC Cardiovascular Disorders](http://bmccardiovascdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bcar/default.aspx","title":"BMC Cardiovascular Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"2ced0fa2-c3df-4720-8da2-049e36fc3d28","owner":[],"postedDate":"December 31st, 2019","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":38127,"name":"Cardiac \u0026 Cardiovascular Systems"},{"id":38128,"name":"Cardiothoracic Surgery"}],"tags":[],"updatedAt":"2021-08-26T17:33:52+00:00","versionOfRecord":{"articleIdentity":"rs-5293","link":"https://doi.org/10.1186/s12872-019-01324-z","journal":{"identity":"bmc-cardiovascular-disorders","isVorOnly":false,"title":"BMC Cardiovascular 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