Protective early innate immune activation by mitochondrial antiviral signalling protein (MAVS) in bacterial pneumonia

Protective early innate immune activation by mitochondrial antiviral signalling protein (MAVS) in bacterial pneumonia | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Protective early innate immune activation by mitochondrial antiviral signalling protein (MAVS) in bacterial pneumonia Timothy Hinks, Yuqing Long, Sophie Morgan, Yonglin Mai, Paul Klenerman This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6368800/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Mitochondrial antiviral signalling protein (MAVS) is important for host antiviral immunity and recent studies suggest its role in fungal defence. However, the role of MAVS in antibacterial host defence remains unclear. The Gram-negative bacterium non-typeable Haemophilus influenzae (NTHi) is the predominant pathogen in airway diseases. Using a murine infection model, we demonstrate that NTHi infection leads to the degradation of MAVS. MAVS deficiency results in compromised bacterial control during NTHi infection. Mavs-/- mice demonstrated impaired early production of proinflammatory cytokines and reduced maturation and activation of NK cells. Depletion of macrophages eliminated the disparity in bacterial loads and weight loss between the two mouse strains. Moreover, in vitro experiments using freshly isolated alveolar macrophages revealed that MAVS played an important role in macrophage function against bacterial infections by detecting bacterial RNA. Overall, our data support the involvement of MAVS signalling in early-stage antibacterial immunity both in vivo and in vitro. Biological sciences/Immunology/Infection Biological sciences/Immunology/Innate immunity Biological sciences/Microbiology/Pathogens MAVS anti-bacterial NTHi innate immunity Asthma Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6368800","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":441197766,"identity":"7123f584-a9fe-4142-9c0e-ce183def7b5f","order_by":0,"name":"Timothy Hinks","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAklEQVRIiWNgGAWjYDACZiBmbGBgMGAGMSvAbCjgwa6DB6TlIFzLGWK0MMC0gCxkbCNCiz07d+LjjzsOM5iz8x42/DnvjuzaBuZjEgw1dgwGZw7gcBjvZoODZw4zWDbzJSfzbntmvO0AW7IBw7FkBoOzDbi0bJM42HaYweAwj/Fhxm2HE7cd4DF8wMB2gMHgPC7vI2k5+HMOWIvBAYZ/RGpJ4G2A2sLYdgC3ww4D/XK2LZ0HpMWY59hh422HgX5J7EvmkcThffb+sxsfVLZZyxmcP2Ms+aPmsOy2483HJD58s5PjO5OA3WUQ0IwUB6DITcAZK3BQR0B+FIyCUTAKRjQAABgUXsTy6wsuAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0003-0699-2373","institution":"University of Oxford","correspondingAuthor":true,"prefix":"","firstName":"Timothy","middleName":"","lastName":"Hinks","suffix":""},{"id":441197767,"identity":"42b0186d-c410-4ea6-bfd9-329bcf3127b6","order_by":1,"name":"Yuqing Long","email":"","orcid":"https://orcid.org/0000-0002-0486-6990","institution":"University of Oxford","correspondingAuthor":false,"prefix":"","firstName":"Yuqing","middleName":"","lastName":"Long","suffix":""},{"id":441197768,"identity":"102164fa-cbae-452f-80df-a5e496b6c2ca","order_by":2,"name":"Sophie Morgan","email":"","orcid":"","institution":"University of Oxford","correspondingAuthor":false,"prefix":"","firstName":"Sophie","middleName":"","lastName":"Morgan","suffix":""},{"id":441197769,"identity":"9b5f9bcf-579d-4fd8-b9e9-627980a9c71d","order_by":3,"name":"Yonglin Mai","email":"","orcid":"https://orcid.org/0000-0002-8070-2701","institution":"University of Oxford","correspondingAuthor":false,"prefix":"","firstName":"Yonglin","middleName":"","lastName":"Mai","suffix":""},{"id":441197770,"identity":"3c08f1ac-dc18-48e7-b718-2c2edf2bbeba","order_by":4,"name":"Paul Klenerman","email":"","orcid":"","institution":"University of Oxford","correspondingAuthor":false,"prefix":"","firstName":"Paul","middleName":"","lastName":"Klenerman","suffix":""}],"badges":[],"createdAt":"2025-04-03 11:15:36","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6368800/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6368800/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":82600160,"identity":"82a16e86-5227-4833-901e-339edb923ee0","added_by":"auto","created_at":"2025-05-13 09:28:51","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":8596027,"visible":true,"origin":"","legend":"Article File","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6368800/v1_covered_bb8e487c-f1ef-4498-a6b0-4ca3644ddf73.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Protective early innate immune activation by mitochondrial antiviral signalling protein (MAVS) in bacterial pneumonia","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"MAVS, anti-bacterial, NTHi, innate immunity, Asthma","lastPublishedDoi":"10.21203/rs.3.rs-6368800/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6368800/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Mitochondrial antiviral signalling protein (MAVS) is important for host antiviral immunity and recent studies suggest its role in fungal defence. 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