Isolation of lipase producing strains Acinetobacter pitti and Enterobacter asburiae from the oil mill sample and induced oil environment and their characterization for Bioremediation

Isolation of lipase producing strains Acinetobacter pitti and Enterobacter asburiae from the oil mill sample and induced oil environment and their characterization for Bioremediation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Isolation of lipase producing strains Acinetobacter pitti and Enterobacter asburiae from the oil mill sample and induced oil environment and their characterization for Bioremediation eswari beeram, Vishnu vardhan S, Siva Priya, SriVidhya This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9467814/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A. pitti & E.asburiae are the gram-negative rod-shaped bacteria isolated from the oil mill sample and induced oil environment by using gram staining method. The isolated bacteria are characterised biochemically using IMVIC test procedures. Both the strains showed positive reaction with methyl red, catalase reaction and citrate utilisation test where as negative for gelatin hydrolysis, urease and oxidase reaction. Enterobacter asburiae is a motile bacterium with motility away from the stab line where as A.pitti is an immotile with motility along the stab line in the semisolid motility agar. Microbial lipases are isolated from the A. pitti & E. asburiae using chromato-focussing and the isoelectric pH of the lipases isolated from the bacteria is found to be 4.4. The isolated lipase is separated using SDS PAGE for the purity assessment. The bacterial strains are confirmed as A. pitti & E. asburiae by 16srRNA sequencing and BLAST assembly. The resulted genomic data is deposited in Gen Bank of NCBI and are provided with the accession numbers PZ167599 (E.asburiae) & PZ167600 (A.pitti). General Microbiology Applied & Industrial Microbiology Enterobacter asburiae Acinetobacter pitti gelatin hydrolysis oxidase reaction catalase reaction SDS PAGE Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9467814","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":626038354,"identity":"5cdc0bf4-479d-4d6c-81fd-29129fff0e1a","order_by":0,"name":"eswari beeram","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1UlEQVRIiWNgGAWjYFACHijN3gAkDCxI0cJzAKRFghQtEglgkrAGfv6zxyQY/tjkGdx8fnXDjwIJBv727gS8WiRn5KVJMLalFRvczim72QN0mMSZsxvwajG4wWMmwdhwOHHD7Zy0GzxALQYSufi12J8/YwZ0GFDLzTNpN/8Qo8WAIQeohQ2o5Qb7sdtE2SJxI8fYIrEtLXHmmRy22zIGEjwE/cLff8bwxoc/Nol9x48/u/nmj40cf3svfi1gkAAmeQzAJGHlCMD+gBTVo2AUjIJRMIIAALtwRn+kHoIUAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-6055-6241","institution":"Mohan babu university","correspondingAuthor":true,"prefix":"","firstName":"eswari","middleName":"","lastName":"beeram","suffix":""},{"id":626038355,"identity":"f07e1aa0-9f81-4fea-aaf0-1084b1b25347","order_by":1,"name":"Vishnu vardhan S","email":"","orcid":"","institution":"Mohan babu university","correspondingAuthor":false,"prefix":"","firstName":"Vishnu","middleName":"vardhan","lastName":"S","suffix":""},{"id":626038356,"identity":"58cde1f5-e2d3-4a06-bd7b-203b29526600","order_by":2,"name":"Siva Priya","email":"","orcid":"","institution":"Mohan babu University","correspondingAuthor":false,"prefix":"","firstName":"Siva","middleName":"","lastName":"Priya","suffix":""},{"id":626038357,"identity":"c8e2da3c-2a03-4e27-95fa-3853fc33cdc8","order_by":3,"name":"SriVidhya","email":"","orcid":"","institution":"Mohan babu university","correspondingAuthor":false,"prefix":"","firstName":"","middleName":"","lastName":"SriVidhya","suffix":""}],"badges":[],"createdAt":"2026-04-20 06:39:39","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-9467814/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9467814/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":107394242,"identity":"d33398a6-35cc-472f-841b-27b472d56a4b","added_by":"auto","created_at":"2026-04-21 06:11:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":681244,"visible":true,"origin":"","legend":"","description":"","filename":"manuscriptfile.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9467814/v1_covered_a3ddfc73-539d-45ab-b00a-f0dd3462d8ac.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eIsolation of lipase producing strains Acinetobacter pitti and Enterobacter asburiae from the oil mill sample and induced oil environment and their characterization for Bioremediation\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Mohan babu University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Enterobacter asburiae, Acinetobacter pitti, gelatin hydrolysis, oxidase reaction, catalase reaction, SDS PAGE","lastPublishedDoi":"10.21203/rs.3.rs-9467814/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9467814/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eA. pitti \u0026amp; E.asburiae are the gram-negative rod-shaped bacteria isolated from the oil mill sample and induced oil environment by using gram staining method. The isolated bacteria are characterised biochemically using IMVIC test procedures. Both the strains showed positive reaction with methyl red, catalase reaction and citrate utilisation test where as negative for gelatin hydrolysis, urease and oxidase reaction. Enterobacter asburiae is a motile bacterium with motility away from the stab line where as A.pitti is an immotile with motility along the stab line in the semisolid motility agar. Microbial lipases are isolated from the A. pitti \u0026amp; E. asburiae using chromato-focussing and the isoelectric pH of the lipases isolated from the bacteria is found to be 4.4. The isolated lipase is separated using SDS PAGE for the purity assessment. The bacterial strains are confirmed as A. pitti \u0026amp; E. asburiae by 16srRNA sequencing and BLAST assembly. 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