A pilot-scale micropropagation plant for two commercial varieties of pineapple Ananas comosus (L.) Merr. reveals shortcuts in a complex system through the recycling of fruit

A pilot-scale micropropagation plant for two commercial varieties of pineapple Ananas comosus (L.) Merr. reveals shortcuts in a complex system through the recycling of fruit | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A pilot-scale micropropagation plant for two commercial varieties of pineapple Ananas comosus (L.) Merr. reveals shortcuts in a complex system through the recycling of fruit Juan Alberto Francisco-Rodríguez, Mauricio Chalchi-Martínez, Héctor López-Arjona, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5313950/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 17 Jan, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 4 You are reading this latest preprint version Abstract Globally, Mexico ranks ninth among the main producers of pineapple (Ananas comosus (L.) Merr.), despite its production system still relying on field-based vegetative propagation, which limits the establishment of new cultivation areas. Consequently, the primary challenge in Mexico is the implementation of in vitro cultivation protocols that enable rapid and effective propagation of pineapples. This study describes a pilot-scale production process for the Cayena Lisa and MD-2 varieties, covering the establishment of in vitro cultures using waste plant material, the propagation of shoots from fruit crowns, acclimatisation, adaptation to the field, and subsequent fruiting in the Papaloapan Basin region, the main production area in Mexico. We evaluated the production of shoots induced with 6-benzylaminopurine (BAP), monitoring the shoot forming capacity (SFC) and the geometric rates of propagation up to the fourth subculture in both semi-solid and liquid Murashige and Skoog (MS) media. In the leaf culture, four types of morphogenic responses were observed: direct organogenesis, rhizogenesis, and both direct and indirect somatic embryogenesis in both varieties. During the rooting stage, we achieved 100% efficiency using indole-3-butyric acid (IBA) or activated charcoal. The follow-up extended to flowering and fruiting in the field, where pineapples were harvested with weights of 1.41 kg (13.9 °Brix) and 1.27 kg (14.43 °Brix). Finally, the procedure was scaled up using temporary immersion bioreactors (TIB) in both normal and double phases, alternating with closed containers. Ananas comosus Cayena Lisa MD-2 mass micropropagation in vitro scaling in vitro germplasm bank Full Text Supplementary Files SupplementaryInformation.pdf Cite Share Download PDF Status: Published Journal Publication published 17 Jan, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Reviewers agreed at journal 05 Nov, 2024 Reviewers invited by journal 04 Nov, 2024 Editor assigned by journal 26 Oct, 2024 First submitted to journal 24 Oct, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5313950","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":373730160,"identity":"03afc64f-3acc-426b-93aa-433c4e5a0ffc","order_by":0,"name":"Juan Alberto Francisco-Rodríguez","email":"","orcid":"","institution":"Universidad del Papaloapan - Campus Tuxtepec","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Juan","middleName":"Alberto","lastName":"Francisco-Rodríguez","suffix":""},{"id":373730161,"identity":"6a2b7409-48d5-41fc-811d-7d9e078fb08d","order_by":1,"name":"Mauricio Chalchi-Martínez","email":"","orcid":"","institution":"Universidad del Papaloapan","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mauricio","middleName":"","lastName":"Chalchi-Martínez","suffix":""},{"id":373730162,"identity":"e36899e8-2c21-4bd5-b5dd-86ece10e1536","order_by":2,"name":"Héctor López-Arjona","email":"","orcid":"","institution":"Universidad del Papaloapan","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Héctor","middleName":"","lastName":"López-Arjona","suffix":""},{"id":373730163,"identity":"f395f2d7-5df9-4268-b94b-4e9750ba683d","order_by":3,"name":"Eugenio Pérez-Molphe-Balch","email":"","orcid":"","institution":"Universidad Autonoma de Aguascalientes","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Eugenio","middleName":"","lastName":"Pérez-Molphe-Balch","suffix":""},{"id":373730164,"identity":"58b9b2f0-ac87-486f-8d7e-a0e168a91ddb","order_by":4,"name":"José Antonio Morales-Serna","email":"","orcid":"","institution":"Universidad del Papaloapan - Campus Tuxtepec","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"José","middleName":"Antonio","lastName":"Morales-Serna","suffix":""},{"id":373730165,"identity":"5421a36f-42d0-48fe-b148-c38c8e710f59","order_by":5,"name":"ENRIQUE VILLALOBOS-AMADOR","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+ElEQVRIiWNgGAWjYFACxsYDDxgsGAyArAdQIQNCWhoOJDBIgJQxw5QS0sLAANPCJkGUFn6JZKAtNRJy5uztz6p5d9yRZ2Bv3ibBmHMYpxbJGYlALcckjC17zpjd5j3zzLCB51iZBOO2NJxaDM4cBGphk0jccCOH7TZv22HGBokcM6AWG5xa7MFa/oG0pD8rBmqxb5B/A9IigVOLAXtjw4HENpCWBDNmoJbEBgke/LZIHAdp6QP7xVhybtuz5DaetGKLRDx+4W9mf/jgwzcbUIg9/PC27Y5tP/vhjTc+bsMdYujgAAMbiEogWgNIyygYBaNgFIwCdAAAP4hYNb5EHTMAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-7411-9114","institution":"Universidad del Papaloapan - Campus Tuxtepec","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"ENRIQUE","middleName":"","lastName":"VILLALOBOS-AMADOR","suffix":""}],"badges":[],"createdAt":"2024-10-22 18:38:50","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5313950/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5313950/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s11240-024-02962-8","type":"published","date":"2025-01-17T15:57:39+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":74284848,"identity":"3ca1bdd2-fd51-426d-a4df-74aa9856a9bb","added_by":"auto","created_at":"2025-01-20 16:13:05","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1403403,"visible":true,"origin":"","legend":"","description":"","filename":"PrincipalManuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5313950/v1_covered_adb9c0c9-7dd7-4fb4-a741-b2f95ca3850d.pdf"},{"id":69108156,"identity":"a150cfa7-da50-4398-9d7a-f4e7fae0c366","added_by":"auto","created_at":"2024-11-15 17:58:09","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1858251,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryInformation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5313950/v1/ad15fb86f1ff123d09b8c404.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eA pilot-scale micropropagation plant for two commercial varieties of pineapple Ananas comosus (L.) 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