Integrating Genetic and Metabolic Approaches to Uncover Heat Stress Tolerance in Wheat

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Abstract

Abstract Wheat, scientifically known as Triticum aestivum , belongs to the Poaceae family. Pakistan aimed to achieve self-sufficiency in wheat production, but the increasing temperature has presented a huge challenge in the form of heat stress. The objective of this study was to characterize wheat genotypes those exhibit heat-tolerance and evaluate the impact of heat stress on metabolic processes. Wheat plants were exposed to high temperatures and phenotypic assessments were performed in a controlled environment. The study was aimed to predict the role of DREB genes associated with heat-tolerance, to investigate interaction and regulatory networks between these DREB and other stress tolerance genes using Bioinformatics techniques. Metabolic profile of wheat was generated using HPLC for significant metabolites related to heat stress were found. The identification of heat tolerance genes and wheat lines possessing more heat tolerance will be used in breeding resources to develop climate-resilient varieties.
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Integrating Genetic and Metabolic Approaches to Uncover Heat Stress Tolerance in Wheat | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Integrating Genetic and Metabolic Approaches to Uncover Heat Stress Tolerance in Wheat Aamir Shehzad, Sehar Nawaz, Muhammad Umar Ikram, Shahid Iqbal, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7515217/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Wheat, scientifically known as Triticum aestivum , belongs to the Poaceae family. Pakistan aimed to achieve self-sufficiency in wheat production, but the increasing temperature has presented a huge challenge in the form of heat stress. The objective of this study was to characterize wheat genotypes those exhibit heat-tolerance and evaluate the impact of heat stress on metabolic processes. Wheat plants were exposed to high temperatures and phenotypic assessments were performed in a controlled environment. The study was aimed to predict the role of DREB genes associated with heat-tolerance, to investigate interaction and regulatory networks between these DREB and other stress tolerance genes using Bioinformatics techniques. Metabolic profile of wheat was generated using HPLC for significant metabolites related to heat stress were found. The identification of heat tolerance genes and wheat lines possessing more heat tolerance will be used in breeding resources to develop climate-resilient varieties. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7515217","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":532117141,"identity":"cbcaa2a0-f14e-4b46-a78b-7508e0362c81","order_by":0,"name":"Aamir 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