Establishing a Multimodal Imaging Tool pProTG  for Selecting and Tracing Cells

Establishing a Multimodal Imaging Tool pProTG for Selecting and Tracing Cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Establishing a Multimodal Imaging Tool pProTG for Selecting and Tracing Cells Yuan Tian, Keyu XIAO, Wenxiu Yang, Peipei Zhang, Yajun Mou, Junhong Zhang, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1651256/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In order to select the cells in vitro, identify the selected cells and trace the selected cells in small animal, we establish a multimodal imaging tool pProTG. We fused enhanced green fluorescent protein, Puromycin N-acetyltransferase and Firefly Luciferase to construct a multimodal imaging tool pProTG, which have ability of selecting the positive cells by puroR, identifying the selected cells by EGFP and imaging the cells in small animal by Fluc. Our established multimodal imaging tool can be used to select the targeted cells in vitro and trace the cells in vivo. Multimodal imaging tool EGFP Puromycin Firefly Luciferase Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1651256","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":105838955,"identity":"0c28d98f-857c-484b-b388-3750cef73a4a","order_by":0,"name":"Yuan Tian","email":"","orcid":"","institution":"","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yuan","middleName":"","lastName":"Tian","suffix":""},{"id":105838956,"identity":"abb95e15-006b-477e-a0f5-4583c05e78d3","order_by":1,"name":"Keyu 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Luciferase","lastPublishedDoi":"10.21203/rs.3.rs-1651256/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1651256/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn order to select the cells in vitro, identify the selected cells and trace the selected\u0026nbsp;cells in small animal, we establish a multimodal imaging tool pProTG. We fused enhanced\u0026nbsp;green fluorescent protein, Puromycin N-acetyltransferase and Firefly Luciferase to construct a\u0026nbsp;multimodal imaging tool pProTG, which have ability of selecting the positive cells by puroR,\u0026nbsp;identifying the selected cells by EGFP and imaging the cells in small animal by Fluc. 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