A Labeled Dataset for AI-based Cryo-EM Map Enhancement

ABSTRACT Cryo-electron microscopy (cryo-EM) has transformed structural biology by enabling near-atomic resolution imaging of macromolecular complexes. However, cryo-EM density maps suffer from intrinsic noise arising from structural sources, shot noise, and digital recording, which complicates accurate atomic structure building. While various methods for denoising cryo-EM density maps exist, there is a lack of standardized datasets for benchmarking artificial intelligence (AI) approaches. Here, we present an open-source dataset for cryo-EM density map denoising comprising 650 high-resolution (1-4 Å) experimental maps paired with three types of generated label maps: regression maps capturing idealized density distributions, binary classification maps distinguishing structural elements from background, and atom-type classification maps. Each map is standardized to 1 Å voxel size and validated through Fourier Shell Correlation analysis, demonstrating substantial resolution improvements in label maps compared to experimental maps. This resource bridges the gap between structural biology and artificial intelligence communities, enabling researchers to develop and benchmark innovative methods for enhancing cryo-EM density maps.