Additional file 1 of Biological characteristics of endometriotic mesenchymal stem cells isolated from ectopic lesions of patients with endometriosis

Additional file 1: Figure S1. Morphology of P3 EnSCs-Control and EnSC-EM-EC. EnSC-EM-EC isolated from independent patient (n = 5) were consistent and showed similar morphology, which display a more “aggressive” phenotype. Figure S2. Paracrine production of biological factors in the CM of EnSCs. The array images of EnSCs-Control (n = 5) and EnSC-EM-EC (n = 6) were shown. Figure S3. Expression of adhesion molecules on EnSCs. The array images of EnSCs-Control (n= 6) and EnSC-EM-EC (n = 7) were shown. Figure S4. GO functional classification on DEGs between EnSC-Control and EnSC-EM-EC.X axis means number of DEGs (the number is presented by its square root value); Y axis represents GO terms. All GO terms are grouped into three ontologies: blue is for biological process; brown is for cellular component and orange is for molecular function. Figure S5. KEGG classification on DEGs between EnSC-Control and EnSC-EM-EC. X axis means number of DEGs; Y axis represents second KEGG pathway terms. All second pathway terms are grouped in top pathway terms indicated in different color. METHODS. Figure S6. Identification of HUVECs. The HUVECs used in tube formation assay positively expressed typical endothelial markers, including CD31, VEGFR2 and vWF, and the positive ration exceeded 95%, which fulfill the standard of endothelial cells. Figure S7. The schematic diagram of CAM assay used in this study with minor improvement. the fertilized chicken eggs were incubated at 38.2°C with approximately 55-65% humidity under sterile conditions. On day 3, the shallow notch was made on the shell with saw blade, and 3 to 5 ml of albumen were removed by sterilized syringe to allow detachment of the developing CAM from the shell. Subsequently, the small hole was sealed with tape, and the eggs were returned to the incubator with the fixed position. On day 7, an opening window was made by scissor on the shell, and a sterilized silicone loop with diameter of 10 mm was placed on top of the growing CAM between mature blood vessels. Table S1. Details of antibodies used. Table S2. The DEGs between EnSC-Control and EnSC-EM-EC. Table S3. The well-chosen top 8 pathway enrichment of DEGs between EnSC-Control and EnSC-EM-EC.