In Vitro and In Vivo Germination of Novel F1 Hybrid Banana Calcutta 4 × Neypoovan Tetraploid Enhanced by Gibberellin and Indole-3-acetic acid

In Vitro and In Vivo Germination of Novel F1 Hybrid Banana Calcutta 4 × Neypoovan Tetraploid Enhanced by Gibberellin and Indole-3-acetic acid | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article In Vitro and In Vivo Germination of Novel F1 Hybrid Banana Calcutta 4 × Neypoovan Tetraploid Enhanced by Gibberellin and Indole-3-acetic acid Bhuvaneshwari - -, Gilbert Ross Rex, Ramajayam - -, Sivashankari - - This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5621344/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This work focuses on rescuing and germinating embryos from F1 hybrid seeds obtained from the cross of Calcutta 4 and Neypoovan tetraploids. The goal was to assess the effects of growth regulators gibberellic acid (GA3) and indole-3-acetic acid (IAA) on seed germination and early seedling development in vitro and in vivo.The seeds were hydro-primed and surface-sterilized for 48 hours before being treated with different concentrations of GA3 (5 and 10 ppm) and IAA (5 and 10 ppm). The seeds were hydro-primed and surface-sterilized for 48 hours before being subjected to various concentrations of GA3 (5 and 10 ppm) and IAA (5 and 10 ppm). Embryo rescue was performed using Murashige and Skoog (MS) media for in vitro germination, with in vivo germination observed for comparison. The results showed that the maximum germination rate (77.78%) and better shoot and root growth were attained under in vitro conditions with GA3 at 5 ppm, with early shoot emergence at 8.40 days and shoot length reaching 6.63 mm. In vivo germination rates, however, were much lower, with just 6.67% germination in seeds treated with GA3 at 5 and 10 ppm and 0% in those treated with IAA. GA3 at 5 ppm was determined to be the best concentration for increasing germination and development of Calcutta 4 x Ney Poovan tetraploid hybrid seeds, especially in vitro. Calcutta 4 X Ney Poovan tetraploid Embryo culture Gibberellin (GA3) Indole-3- acetic acid (IAA) in vitro germination in vivo germination. Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5621344","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":403638753,"identity":"0a5e777a-ed89-4ea9-b75f-face2abe98ce","order_by":0,"name":"Bhuvaneshwari - -","email":"","orcid":"","institution":"NRCB: National Research Centre for Banana","correspondingAuthor":false,"prefix":"","firstName":"Bhuvaneshwari","middleName":"-","lastName":"-","suffix":""},{"id":403638754,"identity":"137a3cc3-e69d-422c-b78e-deb198ebfafe","order_by":1,"name":"Gilbert Ross Rex","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/ElEQVRIiWNgGAWjYDACZuY2ZK4NEDM2HsCvhRGkxQDGTQNpacCvhQFVy2EwiVeLvDtj24OPe/7k8/Mff/bhY9t5u7Xth4G21NhE49JieJix3XDGMwPLmTNyjGfOOHM7eduZRKCWY2m5Dbi0NDO2SfMcMDAwuMHDzMxTcTvZ7ABQC2PDYfxa/oC0nD/+mPmPwblks/MP8WuRB4aYNANIy4EEY2aGigN2ZjcI2GLADPRLzwFjA0mgXxh7ziQnmN0A2pKAxy/y/YePPfhxQM4AGGKPGX622dmbnU9/+OBDjQ1uWw6gCSSCVSbgUA62Bd0sezyKR8EoGAWjYIQCABYtYghbikHDAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-1364-3254","institution":"VCEW: Vivekanandha College of Engineering for Women","correspondingAuthor":true,"prefix":"","firstName":"Gilbert","middleName":"Ross","lastName":"Rex","suffix":""},{"id":403638755,"identity":"64856c46-72d5-47a9-94fb-362c3f3f4aa7","order_by":2,"name":"Ramajayam - -","email":"","orcid":"","institution":"National Research Centre for Banana","correspondingAuthor":false,"prefix":"","firstName":"Ramajayam","middleName":"-","lastName":"-","suffix":""},{"id":403638759,"identity":"10a48d4d-f816-4ee2-b4f1-d22c46c553c3","order_by":3,"name":"Sivashankari - -","email":"","orcid":"","institution":"National Research Centre for Banana","correspondingAuthor":false,"prefix":"","firstName":"Sivashankari","middleName":"-","lastName":"-","suffix":""}],"badges":[],"createdAt":"2024-12-11 06:13:03","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5621344/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5621344/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":84574071,"identity":"dc6fca47-69c0-4a04-84e4-6c8b7b406423","added_by":"auto","created_at":"2025-06-13 16:14:08","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":832983,"visible":true,"origin":"","legend":"","description":"","filename":"Article18.12.24.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5621344/v1_covered_e63f85f1-6390-4a22-b25f-74bd1d20a6e4.pdf"}],"financialInterests":"","formattedTitle":"In Vitro and In Vivo Germination of Novel F1 Hybrid Banana Calcutta 4 × Neypoovan Tetraploid Enhanced by Gibberellin and Indole-3-acetic acid","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Calcutta 4 X Ney Poovan tetraploid, Embryo culture, Gibberellin (GA3), Indole-3- acetic acid (IAA), in vitro germination, in vivo germination.","lastPublishedDoi":"10.21203/rs.3.rs-5621344/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5621344/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This work focuses on rescuing and germinating embryos from F1 hybrid seeds obtained from the cross of Calcutta 4 and Neypoovan tetraploids. The goal was to assess the effects of growth regulators gibberellic acid (GA3) and indole-3-acetic acid (IAA) on seed germination and early seedling development in vitro and in vivo.The seeds were hydro-primed and surface-sterilized for 48 hours before being treated with different concentrations of GA3 (5 and 10 ppm) and IAA (5 and 10 ppm). The seeds were hydro-primed and surface-sterilized for 48 hours before being subjected to various concentrations of GA3 (5 and 10 ppm) and IAA (5 and 10 ppm). Embryo rescue was performed using Murashige and Skoog (MS) media for in vitro germination, with in vivo germination observed for comparison. The results showed that the maximum germination rate (77.78%) and better shoot and root growth were attained under in vitro conditions with GA3 at 5 ppm, with early shoot emergence at 8.40 days and shoot length reaching 6.63 mm. 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