LC-MS and GC-MS -based phytochemical perspectives and antimicrobial effects of Endophytic Fungus Chaetomium ovatoascomatis Isolated from Euphorbia milii

Antimicrobial activity of endophytic fungi isolated from Euphorbia milli was evaluated against Gram positive, Gram negative bacteria, unicellular yeast and filamentous fungi. The most active strain was selected and identified morphologically and genetically as Chaetomium ovatoascomatis NRC . The total ethyl acetate extract of C. ovatoascomatis NRC exhibited a significant antimicrobial activity against Gram negative; Escherichia coli , Salmonella enteric and fungi; Aspergillus niger with MIC of 62.5 ug/ml. While n -hexane fraction demonstrated broader activity against Gram positive; Bacillus subtilis , Lactobacillus cereus , Gram negative; Escherichia coli and Salmonella enteric , fungi; Candida albicans and F. solani . Phytochemical analysis of ethyl acetate strain extract was performed by LCMSMS analysis and n -hexane fraction was analyzed by GCMS. LCMSMS determined five major metabolites with potential antimicrobial activities including coumarin glycoside; esculin and flavonoids. GCMS analysis of n -hexane fraction tentatively identified 30 compounds, where 9,12-octadecadienoic acid methyl ester was the major compound.