Antibacterial and cytotoxic efficacy of Nano- Hydroxyapatite Synthesized from Eggshell and Sheep bones bio Waste

Background: Through a new material pattern addition approach, NHA is synthesized using eggshell materials and sheep bone under different calcination temperatures (respectively at 900 ° C and 800 ° C) using a sol-gel approach. NHA/Es and NHA/Sb, due to their biocompatibility and bioactivity, are widely used in applications such as antibacterial and cytotoxic of PDL cells and teeth and as hard tissue. Results: : We investigated the physicochemical properties of NHA/Es and NHA/Sb by performing FTIR, energy scattering X-ray analysis, SEM, and XRD. The formation of NHA / Es, NHA / Sb particles occurs due to the usage of Eggshell materials and sheep bones as a pattern in NHA synthesis, which are less crystalline. Results of surface morphology analysis of NHA particles indicate that these particles are discrete and rod-shaped at low temperatures (850 °C), whereas larger particles are formed at higher temperatures. Therefore, antibacterial activity against four gram-negative bacteria were investigated through MIC and MBC methods, which included E. coli, Pseudomonas aeruginosa, Candida, Saccharomyces and gram-positive bacteria including Staphylococcus aureus and Enterococcus faecalis. Conclusions: : The reason for the compatibility of calcined NHA / Sb powder as a substance used in biomedical applications is that this powder has shown strong antibacterial efficacy against all bacterial strains with a range of inhibitory zones. The nature and structure of PDLs have been studied in many studies; While we focus on the quantitative analysis of the structural properties of PDL cells and their cytotoxic activity through the use of bio waste materials.