WITHDRAWN: LncRNA HULC-miR-556-5p axis regulates cardiac microvascular endothelial cell function in chronic heart failure through the AMPK/FOXO3 pathway

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Abstract

Abstract Purpose This study aimed to investigate the regulatory mechanism of miR-556-5p in endothelial cell injury associated with heart failure and its impact on endothelial cell function. Methods Human cardiac microvascular endothelial cells (HCMECs) were utilized as the study model. The expression levels of miR-556-5p and related proteins were assessed using techniques such as real-time quantitative PCR and Western blot. Cell apoptosis detection, analysis of inflammatory cytokine release, cell viability assays, and immunoblotting were employed to evaluate cell function and physiological status. Results We observed a crucial role of miR-556-5p in Ang II-induced HCMEC injury. Upregulation of miR-556-5p significantly suppressed cell apoptosis and the release of inflammatory cytokines (such as TNF-α, IL-1β, and IL-6), while promoting cell survival. Further experimental results indicated that miR-556-5p regulated cell function by reducing the expression level of FOXO3 and possibly modulating the AMPK signaling pathway to affect the physiological status of endothelial cells. Additionally, miR-556-5p markedly decreased cellular autophagy levels, further supporting its regulatory role in endothelial cell injury associated with heart failure. Conclusion This study elucidates the important role of miR-556-5p in endothelial cell injury associated with heart failure, providing new insights into the pathophysiological mechanisms of heart failure.
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WITHDRAWN: LncRNA HULC-miR-556-5p axis regulates cardiac microvascular endothelial cell function in chronic heart failure through the AMPK/FOXO3 pathway | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article WITHDRAWN: LncRNA HULC-miR-556-5p axis regulates cardiac microvascular endothelial cell function in chronic heart failure through the AMPK/FOXO3 pathway Yafeng Hao, Mingming Fu, Zhiqian Wang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5749434/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Editorial Note The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference. Editorial notes are used to provide important context regarding the topic of a preprint or to alert readers to potential issues concerning that preprint or a downstream publication associated with it. For more information on editorial notes, see our Editorial Policies . Abstract The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference. Full Text The authors have withdrawn this preprint from Research Square. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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