Effect of metoclopramide to the male rats immune functions and PMN apoptosis after trauma-hemorrhage

Objective:Determine whether administration of metoclopramide(MCP) could produce any effects on the depressed immune function and the delayed apoptosis of PMN after severe hemorrhage.Methods:Male Sprague-Dawley rats(275-325g) were made 5-cm midline laparotomy and bled to a mean arterial pressure of(5±0.5)kPa for 1 h,then randomized into 3 groups: MCP,salinetreated and contrast.2 h after saline or MCP injection,the animals were killed and spleen tissue was homogenated used to determine IL-1β and IL-6.Plasma TNF-α and IL-8 levels were also measured.The Fas expression of PMN was measured too by flow cytometry.Results:Release of IL-1β and IL-6 by spleen tissue in MCP treated group was maintained,whereas salinetreated group rat was depressed.In contrast,there were no difference of plasma lever of TNF-α and IL-8 in MCP group and contrast group, but salinetreated group was higher than the contrast group.The PMN Fas expression of MCP group was lower than the contrast and higher than salinetreated group.Conclusion:The immunosuppression after hemorrhage may be restored by the use of single dose MCP.Furthermore,MCP may be a useful adjuvant in the treatment of the trauma-hemorrhagic shock-induced immunosuppression.