DEVELOPMENT OF PROTOCOLS FOR THE CULTIVATION OF WORMWOOD ( ARTEMISIA SALSOLOIDES )

2025 · doi:10.1101/2025.03.31.646446
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Abstract

ABSTRACT As part of the work, the key factors influencing the growth and development of Artemisia salsoloides culture have been studied, and nutrient media have been developed and optimized for plant conservation. The optimal medium for the cultivation of A. salsoloides is MS + 2 mg/L MT. Viable suspensions were obtained. The MS nutrient medium and the BM1k nutrient medium developed by us are suitable for wormwood callusogenesis. The growth curves of cell suspensions have a standard S-shaped curve. In the A. salsoloides plants we studied, key genes for the synthesis of artemisinin-like compounds (ADS, CYP71AV1, DBR2) were found, except for the CPR gene.
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ABSTRACT As part of the work, the key factors influencing the growth and development of Artemisia salsoloides culture have been studied, and nutrient media have been developed and optimized for plant conservation. The optimal medium for the cultivation of A. salsoloides is MS + 2 mg/L MT. Viable suspensions were obtained. The MS nutrient medium and the BM1k nutrient medium developed by us are suitable for wormwood callusogenesis. The growth curves of cell suspensions have a standard S-shaped curve. In the A. salsoloides plants we studied, key genes for the synthesis of artemisinin-like compounds (ADS, CYP71AV1, DBR2) were found, except for the CPR gene. Competing Interest Statement The authors have declared no competing interest. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

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