Pseudovirus-Mediated Proximity Labeling Identifies Candidate Host Cell Membrane Proteins Involved in Viral Attachment

Abstract Viral infections represent a significant threat to humanity, as exemplified by the COVID-19 pandemic. To mitigate the associated damage, it is essential to understand the biological characteristics of causative viruses. In this study, we report a novel method for identifying host cell molecules (including virus receptor) required for viral attachment, referred to here as “Host Cell Attachment Factors (HCAFs)”. Our approach utilizes proximity labeling (PL) technology: pseudoviruses engineered to express a PL enzyme are applied to host cells, where they bind and initiate proximity labeling. Since HCAFs are expected to be localized near viral attachment sites, candidate HCAFs are specifically tagged by the PL process. Analysis of influenza HCAFs enabled the identification of multiple candidate molecules. Subsequent virus attachment experiments using CHO cells suggested that NRP1 may serve as an HCAF for influenza viruses. Given its simplicity and rapid turnaround, this method is adaptable to a wide range of enveloped viruses, including pandemic viruses that require emergency analysis. Competing Interest Statement The authors have declared no competing interest. Footnotes Abbreviations: EMARS; enzyme-mediated activation of radical source, SARS-CoV-2; severe acute respiratory syndrome coronavirus 2, COVID-19; coronavirus disease 2019, DAF; complement decay-accelerating factor, MS; mass spectrometry, HRP; Horse radish peroxidase, PL; Proximity labeling.