Development of a micro-CT mouse holder for a femur defect mouse model

Correct fixation of an object is essential for accurate micro computed tomography scanning. In this document, we provide a motivation for, description of, and use cases for a mouse holder appropriate for holding an externally fixated mouse in femur fracture/defect healing experiments. In addition to rigid fixation, the holder provides heating and anaesthetic gas to ensure correct anaesthetic conditions for the animal. We provide the description and design files for a Scanco viva40 scanner, but with small changes, the holder can be used with other scanners.