The Expression and Significance of Estrogen Receptor Subtypes in Eutopic Endometrium of Endometriosis and on the Mice Model

Objective To approach the expression and significance of estrogen receptor subtypes(ERα and ERβ) in the eutopic endometrium of patients with endometriosis(EM) and the ectopic focus of on the mice model.Methods 20 cases of EM patients was collected as the EM group and 20 cases of NEM patients(CINⅢ) was collected as control(NEM group).The gradient quantifiable mice model was eatabilished estabilished by implanting the endometrium of 0.2 g,0.4 g and 0.6 g,respectively,and the ectopic focus were obtained 5 days later.The protein expression of ERα and ERβ was detected by immunohistochemistry.Results The implantation rate of the EM group compare to the NEM group:60% and 15% in the 0.2 g group,respectively,there was statistical significance diference between the two groups(P 0.05);100% and 85% in the 0.4 g group and both 100% in the 0.6 g group,there was no statistical significance diference between the two groups(P 0.05).The volume of the ectopic focus in the EM group was larger than that in the NEM group(P 0.05).The expression of ERα in the eutopic endometrium of the EM group was higher than that of the NEM group(P 0.05);In the EM group,the expressions of ERα in the eutopic endometrium was higher than that in the ectopic focus(P 0.05),there was statistical significance diference between the two groups(P 0.05);while in the NEM group,there was no significant difference of the ERα protein expressions between the eutopic endometrium and the ectopic focus.there was no statistical significance diference between the two groups(P 0.05).The protein expression of the ERβ in the eutopic endometrium and the ectopic focus of EM group was higher than them that of the NEM group(P 0.05),respectively,there was statistical significance diference between the two groups(P 0.05),and in both the EM and NEM group,the protein expression of ERβ in the eutopic endometrium was lower than that in the ectopic focus in both the EM and NEM group,there was statistical significance diference between the two groups(P 0.05).Conclusion The eutopic endometrium of EM group has had a much greater implantation capability,the expression of ERα and ERβ are were both higher in eutopic endometrium of the EM group,and the role of the estrogen receptor in the pathogenesis and progresion of the EM was probably determined by the ERβ subtype.