Machine Learning Discoveries of BUB1-X Synergy in ETC-1922159 Treated Colorectal Cancer Cells

The multidomain protein kinase, budding uninhibited by benzimidazoles 1 (BUB1) is a central component of the mitotic checkpoint for spindle assembly (SAC). It works with many different components. A recently developed search engine ranked combi- nations of mitotic checkpoint serine/threonine-protein kinase BUB1-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these rankings point to already confirmed wet lab results, while other point to possible BUB1-X syner- gistic combinations that might be working in CRC, but haven’t been tested/explored. In this research work, I cover combinations of BUB1 with spindle checkpoint pro- tein mitotic arrest deficient 2 (MAD2), inner centromere protein (INCENP), zwilch kinetochore protein (ZWILCH), aurora kinase (AURK), ZW10 interacting kinetochore protein (ZWINT), BUB-1B/3, centromere protein (CENP), cell division cycle (CDC), transforming growth factor beta (TGFB), DNA topoisomerase II (TOP), shugoshin (SGO), kinesin family member (KIF) and cyclin dependent kinase (CDK) family.