Photosynthetic capacity in seagrass seeds and early-stage seedlings ofZostera marinaL

Summary In many terrestrial seeds, photosynthetic activity supplies O 2 to the developing plant embryo to sustain aerobic metabolism and enhance biosynthetic activity. However, whether seagrass seeds possess similar photosynthetic capacity to alleviate intra-seed hypoxic stress conditions is unknown. We used a novel combination of microscale variable chlorophyll fluorescence imaging, a custom-made O 2 optode microrespirometry system, and planar optode O 2 imaging, to determine the O 2 microenvironment and photosynthetic activity in developing seeds and seedlings of seagrass ( Z. marina L.). Developing, sheath-covered seeds exhibited high O 2 concentrations in the photosynthetic active seed sheath and low O 2 concentrations in the center of the seed at the position of the embryo. In light, photosynthesis in the seed sheath increased O 2 availability in central parts of the seed enabling enhanced respiratory energy generation for biosynthetic activity. Early-stage seedlings also displayed photosynthetic capacity in hypocotyl and cotyledonary tissues, which may be beneficial for seedling establishment. Sheath O 2 production is important for alleviating intra-seed hypoxic stress and can increase endosperm storage activity improving the conditions for successful seed maturation and germination.