Hemoglobin Calibration Curves by Photoacoustic Spectroscopy

Hemoglobin Calibration Curves by Photoacoustic Spectroscopy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Hemoglobin Calibration Curves by Photoacoustic Spectroscopy Sindy Janneth Olvera Vazquez, Alfredo Cruz Orea, Sergio Armando Tomás Velázquez This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7530290/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 08 Dec, 2025 Read the published version in International Journal of Thermophysics → Version 1 posted 10 You are reading this latest preprint version Abstract In the present study, hemoglobin calibration curves were developed by photoacoustic spectroscopy (PAS), by using hemoglobin solutions with controlled concentrations. Two optically types of samples were obtained; optically opaque (20–180 mg/mL concentrations) and optically transparent (0.0312–2 mg/ml concentrations). Optical absorption spectra were obtained in the wavelength range from 250 nm to 750 nm. From these spectra the Soret band absorption peak at 412 nm, and 450 nm absorption were identified, and the ratio between these two optical absorptions was obtained. These ratios, as a function of the hemoglobin concentrations, were fitted to the best mathematical model, which allows estimating unknown hemoglobin concentrations from different biological samples, both optically opaque (e.g. blood or organs) and optically transparent (e.g. urine and plasma). The results show a high correlation between the photoacoustic signal and the hemoglobin concentration, validating the applicability of this technique in complex biological systems. Accurate quantification of hemoglobin in biological media is essential for the diagnosis and monitoring of several diseases. PAS is a reliable tool for clinical and biomedical research applications, offering advantages in terms of sensitivity, specificity, and minimal sample volume. Photoacoustic spectroscopy Hemoglobin Calibration curves Optical absorption Biomedical applications Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 08 Dec, 2025 Read the published version in International Journal of Thermophysics → Version 1 posted Editorial decision: Revision requested 24 Oct, 2025 Reviews received at journal 24 Oct, 2025 Reviewers agreed at journal 24 Oct, 2025 Reviews received at journal 26 Sep, 2025 Reviewers agreed at journal 12 Sep, 2025 Reviewers agreed at journal 05 Sep, 2025 Reviewers invited by journal 05 Sep, 2025 Editor assigned by journal 04 Sep, 2025 Submission checks completed at journal 04 Sep, 2025 First submitted to journal 03 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7530290","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":510813833,"identity":"51e5cf92-828b-47a1-a3ac-240c3f557b52","order_by":0,"name":"Sindy Janneth Olvera Vazquez","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABC0lEQVRIiWNgGAWjYDACZgYGCSAlA+Z8ALOJ1MIDYjPOgGhhbCCkC66FmQcigF+LfDvvwds8DDY8Bsd7H362qbHIMzje/vzBzzaGaIMD2LUYHOZLtuZhSOMxOHPcWDrnmESxwZkzho29bQy5M3HYZcDMYybNw3CYR3JGGoN0boNE4swZOYwNPGcYcvtxOawZpmX+M+bfliAt858/bPwD1NKGyzOHoVr4JdjYpBmBWvolGAybeSpw22JwmMfYco5BGg8/TxqbZc8xoBaeHMPZMhUSOP0i33/G8MabChs5NvZjzDd+1NQltrEff/DxjYFN7gYcIQYCTDwGmIL4kwHjD7zSo2AUjIJRMOIBAPuCTs5j4/LIAAAAAElFTkSuQmCC","orcid":"","institution":"CINVESTAV– IPN","correspondingAuthor":true,"prefix":"","firstName":"Sindy","middleName":"Janneth Olvera","lastName":"Vazquez","suffix":""},{"id":510813837,"identity":"739aff7a-f53c-4b9e-9927-231b68712c6c","order_by":1,"name":"Alfredo Cruz Orea","email":"","orcid":"","institution":"CINVESTAV– IPN","correspondingAuthor":false,"prefix":"","firstName":"Alfredo","middleName":"Cruz","lastName":"Orea","suffix":""},{"id":510813838,"identity":"c9b19039-8f1a-4087-a087-d9d9124d1ba1","order_by":2,"name":"Sergio Armando Tomás Velázquez","email":"","orcid":"","institution":"CINVESTAV– IPN","correspondingAuthor":false,"prefix":"","firstName":"Sergio","middleName":"Armando Tomás","lastName":"Velázquez","suffix":""}],"badges":[],"createdAt":"2025-09-03 21:23:08","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7530290/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7530290/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s10765-025-03686-3","type":"published","date":"2025-12-08T15:59:06+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":98244000,"identity":"86157012-1857-4326-9f43-8e337d8378d0","added_by":"auto","created_at":"2025-12-15 16:12:19","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":675121,"visible":true,"origin":"","legend":"","description":"","filename":"ARTICLECALIBRATIONCURVES.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7530290/v1_covered_42b583d3-e812-49f6-ba46-81120f3495e6.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eHemoglobin Calibration Curves by Photoacoustic Spectroscopy\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"international-journal-of-thermophysics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ijot","sideBox":"Learn more about [International Journal of Thermophysics](http://link.springer.com/journal/10765)","snPcode":"10765","submissionUrl":"https://submission.nature.com/new-submission/10765/3","title":"International Journal of Thermophysics","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Photoacoustic spectroscopy, Hemoglobin Calibration curves, Optical absorption, Biomedical applications","lastPublishedDoi":"10.21203/rs.3.rs-7530290/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7530290/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn the present study, hemoglobin calibration curves were developed by photoacoustic spectroscopy (PAS), by using hemoglobin solutions with controlled concentrations. Two optically types of samples were obtained; optically opaque (20\u0026ndash;180 mg/mL concentrations) and optically transparent (0.0312\u0026ndash;2 mg/ml concentrations). Optical absorption spectra were obtained in the wavelength range from 250 nm to 750 nm. From these spectra the Soret band absorption peak at 412 nm, and 450 nm absorption were identified, and the ratio between these two optical absorptions was obtained. These ratios, as a function of the hemoglobin concentrations, were fitted to the best mathematical model, which allows estimating unknown hemoglobin concentrations from different biological samples, both optically opaque (e.g. blood or organs) and optically transparent (e.g. urine and plasma). The results show a high correlation between the photoacoustic signal and the hemoglobin concentration, validating the applicability of this technique in complex biological systems. Accurate quantification of hemoglobin in biological media is essential for the diagnosis and monitoring of several diseases. PAS is a reliable tool for clinical and biomedical research applications, offering advantages in terms of sensitivity, specificity, and minimal sample volume.\u003c/p\u003e","manuscriptTitle":"Hemoglobin Calibration Curves by Photoacoustic Spectroscopy","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-11 18:28:46","doi":"10.21203/rs.3.rs-7530290/v1","editorialEvents":[{"type":"communityComments","content":1},{"type":"decision","content":"Revision requested","date":"2025-10-24T09:21:22+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-24T09:19:18+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"317749139153796084352559924335367340207","date":"2025-10-24T09:18:05+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-26T09:22:26+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"183032427764396534555651538443471424516","date":"2025-09-12T05:59:08+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"333338453894607598920978010847138787332","date":"2025-09-05T13:12:49+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-09-05T09:32:29+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-04T08:24:28+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-04T08:23:32+00:00","index":"","fulltext":""},{"type":"submitted","content":"International Journal of Thermophysics","date":"2025-09-03T21:12:39+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"international-journal-of-thermophysics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ijot","sideBox":"Learn more about [International Journal of Thermophysics](http://link.springer.com/journal/10765)","snPcode":"10765","submissionUrl":"https://submission.nature.com/new-submission/10765/3","title":"International Journal of Thermophysics","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"66dc67c7-c28c-404e-917a-d8da9bef955b","owner":[],"postedDate":"September 11th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-12-15T16:05:13+00:00","versionOfRecord":{"articleIdentity":"rs-7530290","link":"https://doi.org/10.1007/s10765-025-03686-3","journal":{"identity":"international-journal-of-thermophysics","isVorOnly":false,"title":"International Journal of Thermophysics"},"publishedOn":"2025-12-08 15:59:06","publishedOnDateReadable":"December 8th, 2025"},"versionCreatedAt":"2025-09-11 18:28:46","video":"","vorDoi":"10.1007/s10765-025-03686-3","vorDoiUrl":"https://doi.org/10.1007/s10765-025-03686-3","workflowStages":[]},"version":"v1","identity":"rs-7530290","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7530290","identity":"rs-7530290","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}