Salidroside Represses Proliferation of Ovarian Cancer Cells by Heightening Expression Levels of PTEN and ARID1A

Objectives: This study aimed to explore the salidroside influences on apoptosis and proliferation of ovarian cancer SKOV3 cells, also to investigate if the salidroside effects SKOV3 cells via target genes of ARID1A and PTEN. Methods: Ovarian cancer SKOV3 cells cultured in McCoy 5A media were treated with the different concentrations of salidroside (4, 8, 16, 30 and 40 μg/mL), which included SAL-1, SAL-2, SAL-3, SAL-4 and SAL-5 groups, respectively. Flow cytometry was utilized to determine the cell cycle and apoptosis rates of SKOV3 cells. Expression levels of ARID1A and PTEN mRNAs and proteins were detected． Results: In comparison with control group (CG), numbers of SKOV3 cells lessened at 48h and 72h. Inhibition rates of SKOV3 cells proliferation were increased along with the increase of salidroside doses with the largest inhibition rate in SAL-5 (P<0.05). At 24h, 48h and 72h, the apoptosis rates of SKOV3 cells in SAL-5 were significantly higher than those of CG and SAL-1 group (P<0.05 or P<0.01). Expression levels of ARID1A and PTEN mRNAs and proteins were ascended as doses of salidroside increased at 24 h, 48 h and 72 h, respectively. Conclusions: Salidroside could dose-dependently inhibit the proliferation viability of SKOV3 cells, impact the cell division process. Ahigher dose of salidroside promoted the apoptosis of SKOV3 cells, enhanced the expression levels of PTEN and ARID1A mRNAs and proteins.