Agomelatine improves the cognitive function of rats with traumatic brain injury by regulating the polarization of microglia in the hippocampus

Agomelatine improves the cognitive function of rats with traumatic brain injury by regulating the polarization of microglia in the hippocampus | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Agomelatine improves the cognitive function of rats with traumatic brain injury by regulating the polarization of microglia in the hippocampus Lin Yongshuang, Wei Shiyin, Shao Binbin, Huang Yi, LAN Huan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6822178/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective : To explore the effect and mechanism analysis of agomelatine (AGO) in improving cognitive impairment in rats with traumatic brain injury (TBI). Methods : Thirty rats were divided into the Sham group and the TBI group. The TBI group established the TBI rat model by free fall strike. After AGO intervention was conducted on 10 randomly selected rats, the cognitive levels of rats in each group were detected by Morris water maze and field experiments. Result : The protein expressions of TLR4, MyD88 and NF-κB increased, and the expression level of NF-κB in hippocampal tissue also increased (P < 0.05). Conclusion : AGO improves cognitive dysfunction in TBI rats, which may be related to its promotion of M2 polarization of hippocampal microglia, thereby reducing inflammatory injury and inhibiting the TLR4/MyD88/NF-κB pathway. Biological sciences/Neuroscience Biological sciences/Neuroscience/Computational neuroscience Biological sciences/Neuroscience/Oculomotor system Agomelatine Traumatic brain injury Cognitive dysfunction Microglial cell polarization Full Text Additional Declarations No competing interests reported. Supplementary Files arrive.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6822178","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":473496001,"identity":"267f9914-2b62-415e-9068-f58951d72d1e","order_by":0,"name":"Lin Yongshuang","email":"","orcid":"","institution":"The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Lin","middleName":"","lastName":"Yongshuang","suffix":""},{"id":473496002,"identity":"2a899d20-25e2-4cfb-b3e0-74e3d1764287","order_by":1,"name":"Wei Shiyin","email":"","orcid":"","institution":"Affiliated 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hippocampus","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Agomelatine, Traumatic brain injury, Cognitive dysfunction, Microglial cell polarization","lastPublishedDoi":"10.21203/rs.3.rs-6822178/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6822178/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjective\u003c/strong\u003e: To explore the effect and mechanism analysis of agomelatine (AGO) in improving cognitive impairment in rats with traumatic brain injury (TBI).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: Thirty rats were divided into the Sham group and the TBI group. The TBI group established the TBI rat model by free fall strike. After AGO intervention was conducted on 10 randomly selected rats, the cognitive levels of rats in each group were detected by Morris water maze and field experiments.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult\u003c/strong\u003e: The protein expressions of TLR4, MyD88 and NF-κB increased, and the expression level of NF-κB in hippocampal tissue also increased (P \u0026lt; 0.05).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e: AGO improves cognitive dysfunction in TBI rats, which may be related to its promotion of M2 polarization of hippocampal microglia, thereby reducing inflammatory injury and inhibiting the TLR4/MyD88/NF-κB pathway.\u003c/p\u003e","manuscriptTitle":"Agomelatine improves the cognitive function of rats with traumatic brain injury by regulating the polarization of microglia in the hippocampus","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-20 17:22:45","doi":"10.21203/rs.3.rs-6822178/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"91f65016-bbcf-436d-abf9-959b59731578","owner":[],"postedDate":"June 20th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":50284153,"name":"Biological sciences/Neuroscience"},{"id":50284154,"name":"Biological sciences/Neuroscience/Computational neuroscience"},{"id":50284155,"name":"Biological sciences/Neuroscience/Oculomotor system"}],"tags":[],"updatedAt":"2025-10-31T07:01:27+00:00","versionOfRecord":[],"versionCreatedAt":"2025-06-20 17:22:45","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6822178","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6822178","identity":"rs-6822178","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}