Processing of human IVF/IVM oocytes for single cell RNA sequencing
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OA: closed
CC-BY-4.0
Abstract
Abstract The protocol describes the step-by-step procedure of isolating and preparing human oocytes 1) oocytes donated in connection with IVF/ICSI treatment and 2) in vitro matured (IVM) oocytes from small antral follicles donated in connection to fertility preservation. It details how cumulus cells and the zona pellucida are enzymatically removed to ensure that only the oocyte is analyzed. We have successfully used this protocol in connection with SMART-Seq2 (Takara).This oocyte preparation has recently been used in Sankar et al. 2020 (Sankar et al. 2020).
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-06-05T02:00:03.366016+00:00
License: CC-BY-4.0