Study on the effect of recombinant human prolactin on rat endometriosis model

Objective:To explore the effect of recombinant human prolactin (rhPRL) on endometriosis (EM).Methods:50 rat models with EM were established firstly and were randomly divided into study group 1 (10 cases, with 50μg·kg -1 ·d -1 of rhPRL ), group 2 (10 cases, with 100 μg·kg -1 ·d -1 of rhPRL), group 3 (10 cases, with 200μg·kg -1 ·d -1 of rhPRL), Danazol control group (10 cases, with 80 mg·kg -1 ·d -1 of Danazol), saline control group (6 cases) and blank control group (4 cases).Diameter and conglutination of nidus were measured and observed, CD3, CD4 CD8 T lymphocyte was determined by flowcytometry, serum IL-2 and IL-4 were determined by ELISA.Results:CD4/CD8 in group 1 and group 2 were significantly higher than those in saline group and blank controls ( P 0.05).IL-4 levels decreased but IL-2 significantly increased in group 1 and 2 comparing to that in saline group and blank controls ( P 0.05).EM niduser shrinked and conglutination was inhibited in study groups comparing to Danazol controls ( P 0.05) and saline group and blank controls ( P 0.05).Conclusion:The right dosage of rhPRL could play inhibition or treatment role in growth and conglutination of rat EM model by participating in immune regulation.