Regional surround inhibition in mouse sustained On αRGCs depends on GABAergic inputs and light intensity

Regional surround inhibition in mouse sustained On αRGCs depends on GABAergic inputs and light intensity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Regional surround inhibition in mouse sustained On αRGCs depends on GABAergic inputs and light intensity Yanli Ran This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9231946/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Regional properties are common for neurons in the mammalian visual system. In the retina, some neurons change their morphology and light responses with their retinal locations. However, it is unclear whether the region-specific light responses within the same cell type maintains under various light conditions and how these region-specific light responses are generated. Here, we focused on sustained On alpha retinal ganglion cells (sOn αRGCs) which have stronger surround inhibition in the temporal than the nasal retina. By recording light evoked electrical signals in single sOn αRGCs from the temporal and nasal retina, we find that regional difference in surround inhibition present under high photopic conditions but not observable under low photopic conditions. Our pharmacological data show that this regional difference under high photopic conditions relies on GABAergic inputs. Furthermore, we find that the different surround inhibition along the temporal-nasal axis is not present in another RGC type – tOff αRGCs, suggesting that not all RGCs have regional difference in surround inhibition, such as tOff αRGCs. Thus, our data support the notion that the regional surround inhibition in RGCs depend on light stimuli and is type-specific, which might be related to type-specific signal processing under various light conditions. Biological sciences/Neuroscience/Visual system/Retina Biological sciences/Neuroscience/Cellular neuroscience Full Text Additional Declarations There is NO Competing Interest. Supplementary Files WuSupplementalInformation.pdf supplementary material file RS1136.pdf Reporting Summary Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9231946","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":624268018,"identity":"54fb4432-657b-413f-b57b-304d89b6c323","order_by":0,"name":"Yanli Ran","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAtUlEQVRIiWNgGAWjYLCCBxUWYFqCeC0JZyRI1ZLYRooW8/azxyQS50nYGxxgPnibh8Euj6AWmTN5aRKJ2ySYDQ6wJVvzMCQXE9QiwZBjBtLCZnCAx0yah+FAYgNBLfxvgFrmSPAYHOD/RqQWCZAtDRISQFvYiNXyxtgi4ZiEgeRhNmPLOQbJxDgsx/DGhxobe77jzQ9vvKmwI6wFCFgg0cEMIgyIUA9S+4E4daNgFIyCUTBiAQD/vzFPd3Yu5wAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0009-0007-5066-9393","institution":"Lanzhou University","correspondingAuthor":true,"prefix":"","firstName":"Yanli","middleName":"","lastName":"Ran","suffix":""}],"badges":[],"createdAt":"2026-03-26 09:16:49","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9231946/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9231946/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":107705650,"identity":"b039923e-f605-4756-b416-b8ade80ab014","added_by":"auto","created_at":"2026-04-24 09:14:10","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":716441,"visible":true,"origin":"","legend":"Article File","description":"","filename":"MSWusOnRGC.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9231946/v1_covered_67d12635-2a86-49f8-b48b-cf0efdce5b7e.pdf"},{"id":107502691,"identity":"faa35060-eb2c-47c2-a551-6501184846ab","added_by":"auto","created_at":"2026-04-22 06:11:56","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":360423,"visible":true,"origin":"","legend":"supplementary material file","description":"","filename":"WuSupplementalInformation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9231946/v1/11384b45398c29f56953a96b.pdf"},{"id":107502692,"identity":"6c0037d2-840f-473f-ac74-a9e1b048543e","added_by":"auto","created_at":"2026-04-22 06:11:56","extension":"pdf","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":1920485,"visible":true,"origin":"","legend":"Reporting Summary","description":"","filename":"RS1136.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9231946/v1/0ec5581ce5c63ed4bc2a4865.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Regional surround inhibition in mouse sustained On αRGCs depends on GABAergic inputs and light intensity","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-9231946/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9231946/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Regional properties are common for neurons in the mammalian visual system. 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Furthermore, we find that the different surround inhibition along the temporal-nasal axis is not present in another RGC type – tOff αRGCs, suggesting that not all RGCs have regional difference in surround inhibition, such as tOff αRGCs. 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