Abstract
Streptococcus pneumoniae has been reported to produce biofilms that have been linked to antibiotic resistance, stronger immune evasion, and persistent infection. We investigate broth media of different nutritional conditions and supplementation to optimize pneumococcal biofilm formation. The media used were alkaline peptone water (APW), Mueller Hinton broth (MHB), Todd-Hewitt broth supplemented with 0.5% yeast extract (THY), brain heart infusion broth (BHI), and MHB supplemented with 4.5% lysed sheep blood (LSB). Biofilm formation of overnight cells was quantified using the microtiter assay with 0.5% crystal violet at 594 nm. LSB medium was found as the optimal broth medium to induce pneumococcal biofilms. The LSB was then used to classify the biofilm production capacity of different serotypes of pneumococcus. The results show that serotype 3, 20, 11A, 15B, 18C, and non-typable (NT) have a higher biofilm formation capacity. This study provides valuable insight into the influence of different nutrient conditions on pneumococcal biofilm production.
Full text
1,218 characters
· extracted from
oa-doi-fallback
· click to expand
Full text loading...
Abstract
Streptococcus pneumoniae has been reported to produce biofilms that have been linked to antibiotic resistance, stronger immune evasion, and persistent infection. We investigate broth media of different nutritional conditions and supplementation to optimize pneumococcal biofilm formation. The media used were alkaline peptone water (APW), Mueller Hinton broth (MHB), Todd-Hewitt broth supplemented with 0.5% yeast extract (THY), brain heart infusion broth (BHI), and MHB supplemented with 4.5% lysed sheep blood (LSB). Biofilm formation of overnight cells was quantified using the microtiter assay with 0.5% crystal violet at 594 nm. LSB medium was found as the optimal broth medium to induce pneumococcal biofilms. The LSB was then used to classify the biofilm production capacity of different serotypes of pneumococcus. The results show that serotype 3, 20, 11A, 15B, 18C, and non-typable (NT) have a higher biofilm formation capacity. This study provides valuable insight into the influence of different nutrient conditions on pneumococcal biofilm production.
- Received:
- Version Posted:
Funding
-
Badan Riset dan Inovasi Nasional
- Principal Award Recipient: Jesslyn Audrey Virginia
Text is read by the "Ask this paper" AI Q&A widget below.
Extraction quality varies by source — PMC NXML preserves structure
cleanly, OA-HTML may include some navigation residue, and OA-PDF can
have broken hyphenation. The publisher copy
(via DOI)
is the canonical version.