Roseomonas rosulenta sp. nov., isolated from rice paddy soil

Three bacterial isolates, Gram-stain-negative, non-motile, coccobacilli-shaped bacteria, strains OP-27 T , OP-5 and OP-30, were isolated from rice paddy soil. Phylogenetic analyses based on 16S rRNA gene sequences revealed that three isolates belonged to the genus Roseomonas , showing the highest sequence similarities to Roseomonas sediminicola FW-3 T (98.1%), Roseomonas lacus TH-G33 T (98.0%), Roseomonas soli 5N26 T (97.8%), Roseomonas eburnea BUT-5T (97.5%), Roseomonas alkaliterrae YIM 78007 T (97.4%), Roseomonas oryzicola YC6724 T (97.4%), Roseomonas rubea MO17 T (97.4%) and Roseomonas terrae DS-48 T (97.1%) and less than 94.3% similarities to other species of the genus Roseomonas . The genome size of strain OP-27 T was 5.2 Mb in a single contig with DNA G+C content of 71.2%. The genome included 5164 predicted protein-coding genes, as well as 48 tRNA, 4 rRNA and 4 mRNA genes. The average nucleotide identity value between strain OP-27 T and type strains of related species of the genus Roseomonas were 81.1-83.1%, and the digital DNA–DNA hybridization values of strain OP-27 T and the related strains were 24.6~26.8%, respectively. The DNA–DNA hybridization values between strains OP-27 T , OP-5 and OP-30 were 84-100% and its closest relative, Roseomonas sediminicola KACC 16616 T was 21.1%. The major fatty acids were C 18:1 ω 7 c , C 18:1 2-OH and C 16:0 and predominant quinone was Q-10. Based on its distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, the three strains are considered to represent novel species of the genus Roseomonas , for which the name for which the name Roseomonas rosulenta sp. nov. is proposed. The type strain is OP-27 T (=KACC 21501 T =NBRC 114497 T ).