Artificial planting changes soil microbial community dynamics

The artificial planting of grassland serves as the most important means of grassland ecological restoration; however, the impact of artificial planting on soil microbial communities is not well understood. In this study, the evolution of the microbial community structure was studied using 16S and ITS gene sequencing techniques, and the microbial community differences between different forage grasses were analyzed, including different density cropping schemes, multi-year degraded grassland and natural grassland. It was found that the high-density planting scheme of multiple pastures exerts a great impact on soil nutrients as well as on the soil microbial community, effectively increasing the relative abundance of Actinobacteria and Basidiomycota, while the microbial community structure was found to be similar to that of natural grassland. However, in artificial planting treatment, the key node microflora group was noted to be bacteria, which was different from that in natural grassland, in which the key node microflora group was fungi. In comparison, fungi were found to be more sensitive than bacteria to different plantings.The rise in soil fungal diversity did not improve phosphate mineralization.Overall, this study may contribute to understanding the influence of artificial grassland on soil properties as well as the succession of microbial communities, How to accelerate the succession process of grassland ecosystem. which are of great significance in promoting artificial technology to restore the ecological environment.