Investigating the influence of extraction methodology, and certain other experimental parameters on bacterial response to selected quorum-modulatory plant extracts

In context of the global threat of antimicrobial resistance (AMR), a large number of labs throughout the world are investigating plant extracts for their possible anti-pathogenic efficacy. Outcome of protocols employed for screening the plant extracts for such activities are influenced by multiple factors. Among them, this study has found the method used for extraction, size and volume of the assay system, to be important factors. Further we demonstrate the extracts prepared by microwave-and vacuum-assisted extraction methods to be stable with respect to their anti-pathogenic efficacy over a long period of storage.