Propagation through seed or somatic embryogenesis: Comparing the effects of two methods and methyl jasmonate treatment on Norway spruce resistance to Heterobasidion infection

Propagation through seed or somatic embryogenesis: Comparing the effects of two methods and methyl jasmonate treatment on Norway spruce resistance to Heterobasidion infection | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Propagation through seed or somatic embryogenesis: Comparing the effects of two methods and methyl jasmonate treatment on Norway spruce resistance to Heterobasidion infection Amelia Tudoran, Malin Elfstrand, Andreas Helmersson, Adriana Puentes This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5355564/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Dec, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 4 You are reading this latest preprint version Abstract The clonal propagation method somatic embryogenesis (SE) has been shown to affect plant resistance to an insect pest. In a field trial, Norway spruce SE-plants (emblings) experienced less damage by the bark-chewing pine weevil compared to seedlings propagated through seeds from the same mother tree. Moreover, if emblings were treated with the defense-inducing hormone methyl jasmonate (MeJA), their resistance became much greater compared to the effects of SE and MeJA alone. Thus, we evaluated in a full factorial design if propagation method (seed or SE) and MeJA treatment (treated or not) can affect Norway spruce resistance to infection by the fungal pathogen Heterobasidion parviporum. Emblings and seedlings were half-sibs originating from four different Norway spruce families. We found that emblings and seedlings exhibited similar fungal lesion lengths (LL) and sapwood growth (SWG) in a constitutive state (no MeJA). Once treated, seedlings exhibited a 26% and 28% reduction in LL and SWG growth respectively, in line with previous studies. For emblings, it was the opposite. MeJA increased LL and SWG by 41% and 16% respectively. This is unexpected given the genetic relatedness of the material, and the previously documented effects of SE. It is possible that SE is causing changes in Norway spruce resistance that are effective against insects, but not pathogens. Also, MeJA treatment may be affecting embling terpene composition in ways that benefit fungal growth, as suggested from separate experiments. Further studies are needed to uncover the mechanisms behind the increased susceptibility of Norway spruce emblings to fungal infection. clonal propagation conifers fungal susceptibility methyl jasmonate Full Text Supplementary Files TudoranetalSupplementaryinformation.pdf Cite Share Download PDF Status: Published Journal Publication published 10 Dec, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Reviewers agreed at journal 04 Nov, 2024 Reviewers invited by journal 04 Nov, 2024 Editor assigned by journal 30 Oct, 2024 First submitted to journal 29 Oct, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5355564","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":373723489,"identity":"35ba7080-7255-48d5-8d93-6a1fbfa69205","order_by":0,"name":"Amelia 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