High throughput spectroscopy of pL droplets

High throughput spectroscopy of pL droplets | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article High throughput spectroscopy of pL droplets Jaime Ortega Arroyo, Marc Sulliger, Romain Quidant This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4023751/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet microfluidics with sensitive, broadband spectroscopic techniques would therefore unlock new capabilities for various disciplines, including biomedicine and biochemistry. Here we present an integrated optofluidic platform that seamlessly combines droplet microfluidics and advanced hyperspectral imaging. This enables high-resolution, label-free analysis of single picolitre-sized droplets, providing valuable insights into their content. As a proof-of-principle, we demonstrate the ability of our platform to study rapid dynamic changes in a heterogeneous population of plasmonic nanoparticles with millisecond-time resolution. Furthermore, we demonstrate the effectiveness of the platform in biosensing applied to short DNA strands, achieving a detection sensitivity in the range of 100 pM. Finally, we show that the platform provides the flexibility to monitor samples over extended periods of time (hours) in a multiplexed manner. Physical sciences/Nanoscience and technology/Techniques and instrumentation/Characterization and analytical techniques Biological sciences/Biological techniques/Lab-on-a-chip Physical sciences/Nanoscience and technology/Nanobiotechnology/Microfluidics Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4023751","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":281476325,"identity":"a4115848-883f-4238-ab05-28750b5ecf1a","order_by":0,"name":"Jaime Ortega Arroyo","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAt0lEQVRIiWNgGAWjYBACxgYILcfAwAOiDxCvxZiBjVgtMJDYQLQW5vazzz58bLNJ75fvPfyBoeYOEQ7rSTeeObMtLXdmG1+CAcOxZ0RoaUhjZuZtO5y74RiPQQJjw2EitPQ/A2tJtwdqOUCclhkQWxIM2HgMG4jU8oyZcca5NMMZx3KMGRKOEaHFsD+NmeFDmY08f/MZ4w8faojR0gCyig3KSyCsgYFBHkz+IUbpKBgFo2AUjFgAADPbN/bctlOpAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-0657-051X","institution":"ETH Zurich","correspondingAuthor":true,"prefix":"","firstName":"Jaime","middleName":"Ortega","lastName":"Arroyo","suffix":""},{"id":281476326,"identity":"ec3e95f4-1b7c-41f3-8152-db27db866bfb","order_by":1,"name":"Marc Sulliger","email":"","orcid":"https://orcid.org/0000-0002-2706-3912","institution":"ETH Zurich","correspondingAuthor":false,"prefix":"","firstName":"Marc","middleName":"","lastName":"Sulliger","suffix":""},{"id":281476327,"identity":"5bb8c9ed-6ab9-4fb1-b7ec-439ec812893a","order_by":2,"name":"Romain Quidant","email":"","orcid":"https://orcid.org/0000-0001-8995-8976","institution":"ETH Zurich","correspondingAuthor":false,"prefix":"","firstName":"Romain","middleName":"","lastName":"Quidant","suffix":""}],"badges":[],"createdAt":"2024-03-07 09:15:40","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4023751/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4023751/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":61883178,"identity":"73a7d94e-335f-46a9-8eca-12a3e5022854","added_by":"auto","created_at":"2024-08-06 15:55:11","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1005449,"visible":true,"origin":"","legend":"","description":"","filename":"Sulliger2024NatCommunManuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4023751/v1_covered_de2bd549-5a81-418c-a58a-3a7fcc5a289f.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"High throughput spectroscopy of pL droplets","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4023751/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4023751/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Droplet microfluidics offers a versatile platform for analyzing liquid samples. 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