Using Manganese-Enhanced MRI to visualize Magnetogenetic-based Neuromodulation

Purpose Investigation of the electromagnetic perceptive gene (EPG) protein and garnering evidence to suggest its use as a magnetogenetic tool for neuromodulation. Activation of EPG by electromagnetic field increases intracellular calcium levels, thus, we aimed to determine whether EPG influences the analogous manganese ion. This work yields potential for manganese-enhanced MRI (MEMRI) to be used to monitor EPG acting as a neuromodulator. Methods HEK293FT cells expressing EPG were exposed to a MnCl 2 solution and stimulated with a static or electromagnet. Excess MnCl 2 was washed off the cells, followed by their collection and lysis. T 1 map measurements of the lysates were obtained to gauge the presence of intracellular Mn 2+ . Several controls were employed to critically evaluate whether EPG can influence Mn 2+ dynamics. Results Lysate of cells expressing EPG showed significantly lower T 1 values compared to cells without EPG that were exposed to the same MnCl 2 solution and magnetic stimulus. Conclusion Magnetic activation of EPG increases the uptake of Mn 2+ into the cell. By influencing ions pertinent to neuronal function, we demonstrate the potential of MEMRI to monitor EPG neuromodulatory activity.