Role of HPV 16 variants among cervical samples from Northeastern Brazil

Role of HPV 16 variants among cervical samples from Northeastern Brazil | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research article Role of HPV 16 variants among cervical samples from Northeastern Brazil Rodrigo Lopes da Silva, Zulmira da Silva Batista, Gerusinete Rodrigues Bastos, and 12 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.2.20515/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Cervical cancer is the fourth most common type of cancer affecting women globally. In Brazil, it is the third most frequent type of cancer in women and HPV is present in approximately 90% of cases. Evidence suggests that variants of HPV 16 can interfere biologically and etiologically during the development of cervical cancer. Methods: Cervix tumor fragments were collected, their DNA was extracted, and nested PCR was used to detect HPV. Positive samples were sequenced to determine the viral genotype. To characterize the HPV 16 strains, positive samples PCR was used to amplify the LCR and E6 regions of the HPV 16 virus. Results: Data from 120 patients with cervical cancer were analyzed. Most women were between 41 and 54 years of age, had schooling until primary school, a family income between 1 and 2 times the minimum wage and were married/in a consensual union. There was no statistically significant association between HPV or socio-demographic variables and risk factors for cervical cancer (P < 0.05). HPV was present in 88 women (73%). The most prevalent types were HPV 16 (47/54%), HPV 18 (12/13.8%), HPV 35 (6/6.9%) and HPV 45 (5/5.7%). Of the 47 HPV 16 positive cases, variant A (49%) was present in 23 samples, followed by variant D in 20 cases (43%), and variants B and C in 2 cases each (4%).The most prevalent histological type of HPV 16 tumors was epidermoid carcinoma, followed by adenocarcinoma. There was a statistically significant association between HPV 16 variants and the tumors’ histological types (P < 0.001). Conclusions: Knowledge of HPV 16 variants will provide data on their influence on the pathological and oncogenic aspects of cervical lesions. Oncology Cervical cancer Human papillomavirus HPV 16 Variants Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-11119","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":286218,"identity":"482e506b-f7d9-4fca-a1df-1c5f324f0d26","order_by":1,"name":"Rodrigo Lopes da Silva","email":"","orcid":"","institution":"Universidade Federal do Maranhao","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Rodrigo","middleName":"Lopes da","lastName":"Silva","suffix":""},{"id":286219,"identity":"cb6c34cc-c5d1-4a9f-8600-b9892aa684c3","order_by":2,"name":"Zulmira da Silva Batista","email":"","orcid":"","institution":"Universidade 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Lacerda","lastName":"Barbosa","suffix":""},{"id":286231,"identity":"7ce26862-86d4-4321-b80e-0236fcbd9d70","order_by":14,"name":"Miguel Ângelo Martins Moreira","email":"","orcid":"","institution":"Instituto Nacional de Cancer","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Miguel","middleName":"Ângelo Martins","lastName":"Moreira","suffix":""},{"id":286232,"identity":"f3292b82-7ca9-45a8-ab07-6487a6a17264","order_by":15,"name":"Maria do Desterro Soares Brandão Nascimento","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAwElEQVRIiWNgGAWjYJACCSCWY2BgbgDzDIjVYszAwEiilsQGorXwz25/eIMxxy59w/HGBoYfFdsYzKUPELDhzhljC8ZtybkbzhxsYOw5c5vBsi8BvxYDiRw2CcZtzLkbbiQ2MDO23WYwOEPAYQYS6c+AWurTDUjQkmAG1HI4gXgtEjdyQH45bjgT6JeDQL/wWPYQ0MI/Ix0YYtuq5fmONx988KPitpw5DwEtIMD8B8o4AMTEaBgFo2AUjIJRQAgAAKeRQeARB4HlAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0003-2783-362X","institution":"Universidade 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Variants","lastPublishedDoi":"10.21203/rs.2.20515/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.2.20515/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground: Cervical cancer is the fourth most common type of cancer affecting women globally. In Brazil, it is the third most frequent type of cancer in women and HPV is present in approximately 90% of cases. Evidence suggests that variants of HPV 16 can interfere biologically and etiologically during the development of cervical cancer. \u003c/p\u003e\u003cp\u003eMethods: Cervix tumor fragments were collected, their DNA was extracted, and nested PCR was used to detect HPV. Positive samples were sequenced to determine the viral genotype. To characterize the HPV 16 strains, positive samples PCR was used to amplify the LCR and E6 regions of the HPV 16 virus.\u003c/p\u003e\u003cp\u003eResults: Data from 120 patients with cervical cancer were analyzed. Most women were between 41 and 54 years of age, had schooling until primary school, a family income between 1 and 2 times the minimum wage and were married/in a consensual union. There was no statistically significant association between HPV or socio-demographic variables and risk factors for cervical cancer (P \u0026lt; 0.05). HPV was present in 88 women (73%). The most prevalent types were HPV 16 (47/54%), HPV 18 (12/13.8%), HPV 35 (6/6.9%) and HPV 45 (5/5.7%). Of the 47 HPV 16 positive cases, variant A (49%) was present in 23 samples, followed by variant D in 20 cases (43%), and variants B and C in 2 cases each (4%).The most prevalent histological type of HPV 16 tumors was epidermoid carcinoma, followed by adenocarcinoma. There was a statistically significant association between HPV 16 variants and the tumors’ histological types (P \u0026lt; 0.001).\u003c/p\u003e\u003cp\u003eConclusions:\u0026nbsp;Knowledge of HPV 16 variants will provide data on their influence on the pathological and oncogenic aspects of cervical lesions.\u003c/p\u003e","manuscriptTitle":"Role of HPV 16 variants among cervical samples from Northeastern Brazil","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2020-01-10 16:02:44","doi":"10.21203/rs.2.20515/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b85f3faf-5f1b-472a-adc1-48cba1c8c98b","owner":[],"postedDate":"January 10th, 2020","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":48581,"name":"Oncology"}],"tags":[],"updatedAt":"","versionOfRecord":[],"versionCreatedAt":"2020-01-10 16:02:44","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-11119","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"identity":"rs-11119","version":["v1"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}