Effect of pregnancy on cell proliferation in endometriotic lesions.

The percentage of proliferating cells was assessed by immunohistochemistry for PCNA in epithelial (A) and stromal (B) cells from pregnant (N = 11) and non pregnant (N = 9) mice. Micrographs (C-E) show representative histological sections of endometriotic lesions from pregnant (C) and non pregnant (D) mice. Arrows indicate marked cells. As a negative control, immunoglobulin of the same immunoglobulin class and concentration as the primary antibody was used (E). Scale bar indicates 100 μm. Statistical comparisons were performed by Student “t” test. * p<0.05 pregnant vs. non pregnant.