Syzygium caryophyllatum derived silver nanoparticles enhance GLUT4 expression via AMPK-associated signaling in adipocytes

Syzygium caryophyllatum derived silver nanoparticles enhance GLUT4 expression via AMPK-associated signaling in adipocytes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Syzygium caryophyllatum derived silver nanoparticles enhance GLUT4 expression via AMPK-associated signaling in adipocytes Santosh Mallikarjun Bhavi, Ramesh Babu Yarajarla This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8916619/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 17 You are reading this latest preprint version Abstract Adipocyte insulin responsiveness depends on AMPK signaling and GLUT4 trafficking, yet biogenic silver nanoparticle (AgNP) effects remain underexplored. This study extends prior synthesis/characterization of Syzygium caryophyllatum-mediated SC-AgNPs (21.78 nm TEM, λmax 421 nm) to 3T3-L1 adipocyte signaling. The identical batch, verified stable by UV-Vis and visual inspection, was tested across viability (MTT), ROS (DCFDA), mitochondrial function (TMRE), apoptosis (Annexin V/PI), and AMPK/GLUT4 regulation (endpoint RT-PCR, Western blot) using vehicle controls. SC-AgNPs showed excellent biocompatibility (IC50 605.8 μg/mL, >95% viability at 5-50 μg/mL) with hormetic bioactivity: controlled ROS elevation (1.3-fold), mitochondrial hyperpolarization (2.21-fold), versus apoptosis at IC50 (91.7%). At sub-toxic working concentrations, GLUT4 mRNA/protein increased 1.55/1.6-fold (GAPDH/β-actin normalized) despite AMPK reduction (0.2-0.7-fold), suggesting post-translational AMPK-associated signaling. These findings demonstrate SC-AgNPs predict insulin-independent GLUT4-mediated glucose handling via redox modulation, establishing nanoparticle-specific metabolic effects for future functional validation (p-AMPK, GLUT4 translocation, glucose uptake assays). Full Text Additional Declarations No competing interests reported. Supplementary Files SupplemenatryData.docx GraphicalAbstract.tif Fig.S1.tif Fig.S2.tif Fig.S3.tif Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 02 May, 2026 Reviews received at journal 02 May, 2026 Reviews received at journal 01 May, 2026 Reviews received at journal 26 Apr, 2026 Reviewers agreed at journal 25 Apr, 2026 Reviewers agreed at journal 24 Apr, 2026 Reviewers agreed at journal 23 Apr, 2026 Reviewers agreed at journal 23 Apr, 2026 Reviews received at journal 23 Apr, 2026 Reviewers agreed at journal 23 Apr, 2026 Reviews received at journal 03 Apr, 2026 Reviewers agreed at journal 26 Mar, 2026 Reviewers agreed at journal 25 Mar, 2026 Reviewers invited by journal 25 Mar, 2026 Editor assigned by journal 04 Mar, 2026 Submission checks completed at journal 03 Mar, 2026 First submitted to journal 19 Feb, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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