Derivation of ringed seal (Phoca hispida) induced multipotent stem cells

Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal ( Phoca hispida ) fibroblasts. The resulting cell line had the expression of pluripotency marker gene Rex1 . Differentiation in embryoid body-like structures allowed us to register expression of AFP , endoderm marker, and Cdx2 , trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes.