Intraspecific variation in temperature and phosphorus dependence of growth rates: hints of local adaptation in a freshwater diatom

Intraspecific variation in temperature and phosphorus dependence of growth rates: hints of local adaptation in a freshwater diatom | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Ecology and Evolution This is a preprint and has not been peer reviewed. Data may be preliminary. 16 April 2025 V1 Latest version Share on Intraspecific variation in temperature and phosphorus dependence of growth rates: hints of local adaptation in a freshwater diatom Authors : Li Zhao 0009-0009-9358-2663 , Divina Ryf , Sarah. Levasseur , Raphael Bossart , Marta Reyes , Frank Pennekamp 0000-0003-0679-1045 , Jukka Jokela , Anita Narwani 0000-0003-4561-0163 , and Vanessa Weber de Melo 0000-0001-9558-4042 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174480128.80490313/v1 404 views 160 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Climate change is reshaping freshwater ecosystems by warming waters and modifying nutrient dynamics. These combined environmental changes exert novel gradients of selection on phytoplankton populations and communities. Temperature and phosphorus availability are individually critical determinants of growth in phytoplankton, and can have interactive impacts on population and community dynamics. While we understand how interspecific variation in thermal and resource-use traits of phytoplankton can affect community composition in response to changing environments, the extent of intraspecific variation in these responses remains poorly understood. In this study, we examined the intraspecific variation in the temperature- and phosphorus-dependences of growth in the freshwater diatom Fragilaria crotonensis . We predict that the growth of this diatom is locally adapted to the environmental conditions of the lakes of origin. To test this, we isolated strains from eight Swiss lakes (one strain per lake) with distinct historical temperatures and nutrient status. We estimated the growth rate of each strain under combined gradients of temperature and phosphorus availability. We fitted Monod curves to the growth rate data, and we quantified the minimal phosphorus requirements ( P *), half-saturation constants ( K s ) and maximum growth rates (μ max ) for each strain as a function of temperature. We also fitted thermal performance curves and quantified activation energies ( E a ) and cumulative performance across the thermal gradient as a function of phosphorus availability. We observed large intraspecific variation on the dependency of P* on experimental temperature, with strains from phosphorus-rich lakes showing stronger increases in phosphorus requirements with warming. These patterns imply local adaptation to phosphorus availability. Our findings highlight a potentially critical role for intraspecific diversity and local adaptation in shaping phytoplankton responses to global change and call for a greater recognition of this trait variation in making predictions of community-level responses to future climate. Supplementary Material File (manuscript.docx) Download 3.52 MB File (tables.docx) Download 19.97 KB Information & Authors Information Version history V1 Version 1 16 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Ecology and Evolution Keywords comparative ecological experiment evolutionary ecology freshwater laboratory microbial Authors Affiliations Li Zhao 0009-0009-9358-2663 Eawag View all articles by this author Divina Ryf Eawag View all articles by this author Sarah. Levasseur Eawag View all articles by this author Raphael Bossart Eawag View all articles by this author Marta Reyes Eawag View all articles by this author Frank Pennekamp 0000-0003-0679-1045 University of Zurich View all articles by this author Jukka Jokela Eawag View all articles by this author Anita Narwani 0000-0003-4561-0163 Eawag View all articles by this author Vanessa Weber de Melo 0000-0001-9558-4042 [email protected] Eawag View all articles by this author Metrics & Citations Metrics Article Usage 404 views 160 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Li Zhao, Divina Ryf, Sarah. Levasseur, et al. Intraspecific variation in temperature and phosphorus dependence of growth rates: hints of local adaptation in a freshwater diatom. Authorea . 16 April 2025. DOI: https://doi.org/10.22541/au.174480128.80490313/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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