Yeast–Plant-Derived Consortia Factors Exhibit A Distinct And Enriched Beneficial Metabolome For Health And Beauty

Abstract Cells secrete metabolites and other factors into the extracellular medium, collectively referred to as the secretome. The effect of co-culturing cells from different species on their combined secretome remains poorly understood. Here, we investigated the effect of co-culturing plant and yeast cells to produce a unique set of secretory factors collectively termed Consortia Factors (CFx). Specifically, a yeast suspension of Ustilago cynodontis (Ustilago) was co-cultured with plant cells derived from Ocimum sanctum (Tulsi). The metabolome of the Ustilago-Tulsi CFx (termed CFx-α1) was then profiled and compared with that of the individual cultures. Statistical clustering analyses revealed that the CFx-α1 metabolome was substantially different from either culture grown alone and was enriched in metabolites relative to single cultures. Notably, significant enrichment was observed among metabolites mutually upregulated in CFx compared with both single cultures, with approximately one-third absent from either culture alone. Using an algorithm designed to identify scientifically validated antioxidant metabolites, the CFx-α1 was found to be enriched in antioxidants relative to the single cultures, including vitamin C. Accordingly, the CFx-α1 exhibited stronger antioxidant activity than either of the single-culture secretomes, and even than the combination of the two single-culture secretomes. These findings suggest that consortia factors derived from co-culturing yeast and plant cells can generate a unique and more potent secretome that is enriched in bioactive metabolites beneficial for human health. Competing Interest Statement The Authors are employed by Rinati Skin LLC, who is the holder of the patent US11473117B2, and funded the research and drafting of this article. Rinati Skin conducts research and development for ingredients used in skincare and personal care products.