In situ new variations in Puccinia triticina causing leaf rust on wheat

In situ new variations in Puccinia triticina causing leaf rust on wheat | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article In situ new variations in Puccinia triticina causing leaf rust on wheat Subodh Kumar, Subhash Chander Bhardwaj, Om Prakash Gangwar, Pramod Prasad, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-945016/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract During the periodic purity checks of pathotypes 107-1(45R35=JCGPL) and 20(5R27=SGQPL) of Puccinia triticina in national repository at Shimla, India, mixtures of infection types were observed on the differential Lr3 and Lr15 , respectively. Single pustule isolations and further pure cultures in both the cases yielded new pathotypes designated as 57(45R39= KGTPL) and 20-2(57R27= SHKPL). Pathotype 57 was one step gain in virulence on Lr3 in 107-1 whereas 20-2 on Lr15 in mother culture of pathotype 20. The difference of virulence on one gene to each mother culture and their non occurrence in the field samples is indicative of mutation for virulence in situ . To ascertain the novelty of new pathotypes, detailed study on differentials, avirulence/virulence structure, DNA polymorphism using SSR markers, and other related information is presented in this publication. The new cultures are being maintained as PrtI 57 and PrtI 20-2 in the repository. Agroecology Evolutionary Biology Plant Molecular Biology and Genetics Puccinia triticina Wheat leaf rust Mutation in culture Virulence New pathotypes Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-945016","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":60163853,"identity":"40c5782b-ba11-4b4e-9b33-91a53a74314a","order_by":0,"name":"Subodh Kumar","email":"","orcid":"","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Subodh","middleName":"","lastName":"Kumar","suffix":""},{"id":60163854,"identity":"097e1b57-6852-4ab4-8136-6f1e9271fa3f","order_by":1,"name":"Subhash Chander Bhardwaj","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAv0lEQVRIiWNgGAWjYFACHoYPPAU2CWB2QgFxWhhn8BikJTCwgbQYEK/lMEQLAzFa5NvPHmx4Y3A+j1++O/HDAwMGeX6xA/i1GJzJS2ycY3C7WLKNd7ME0GGGM2cnENAiwWP+mMfgduKGY7wbQFoSDG4T0CI/g8ewmcfgHEjL5h9EaWG4AdZyAKRlG3G2QP2SnDizLXebRYKBBGG/QEKswi6xn/ns5ps/Kmzk+aUJOQwNSJCmfBSMglEwCkYBdgAAYwJDI9C7X+gAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-4070-8412","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Subhash","middleName":"Chander","lastName":"Bhardwaj","suffix":""},{"id":60163855,"identity":"924e82a6-33f4-41a1-bfea-5ba254e94abb","order_by":2,"name":"Om Prakash Gangwar","email":"","orcid":"","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Om","middleName":"Prakash","lastName":"Gangwar","suffix":""},{"id":60163856,"identity":"7f734a73-d9ab-41ab-b797-33842c39a047","order_by":3,"name":"Pramod Prasad","email":"","orcid":"","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Pramod","middleName":"","lastName":"Prasad","suffix":""},{"id":60163857,"identity":"38818d38-7b91-45fe-a621-cd411ea38892","order_by":4,"name":"Charu Lata","email":"","orcid":"","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Charu","middleName":"","lastName":"Lata","suffix":""},{"id":60163858,"identity":"5ca36eb7-7cf0-4328-83a2-6276a3f5f4ea","order_by":5,"name":"Sneha Adhikari","email":"","orcid":"","institution":"ICAR - Indian Institute of Wheat and Barley Research","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Sneha","middleName":"","lastName":"Adhikari","suffix":""}],"badges":[],"createdAt":"2021-09-28 14:43:09","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-945016/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-945016/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[],"financialInterests":"","formattedTitle":"\u003cp\u003e\u003cem\u003eIn situ\u003c/em\u003e new variations in \u003cem\u003ePuccinia triticina\u003c/em\u003e causing leaf rust on wheat\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-945016/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Puccinia triticina, Wheat leaf rust, Mutation in culture, Virulence, New pathotypes","lastPublishedDoi":"10.21203/rs.3.rs-945016/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-945016/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"During the periodic purity checks of pathotypes 107-1(45R35=JCGPL) and 20(5R27=SGQPL) of Puccinia triticina in national repository at Shimla, India, mixtures of infection types were observed on the differential Lr3 and Lr15 , respectively. Single pustule isolations and further pure cultures in both the cases yielded new pathotypes designated as 57(45R39= KGTPL) and 20-2(57R27= SHKPL). Pathotype 57 was one step gain in virulence on Lr3 in 107-1 whereas 20-2 on Lr15 in mother culture of pathotype 20. The difference of virulence on one gene to each mother culture and their non occurrence in the field samples is indicative of mutation for virulence in situ . To ascertain the novelty of new pathotypes, detailed study on differentials, avirulence/virulence structure, DNA polymorphism using SSR markers, and other related information is presented in this publication. The new cultures are being maintained as PrtI 57 and PrtI 20-2 in the repository.","manuscriptTitle":"In situ new variations in Puccinia triticina causing leaf rust on wheat","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-11-01 15:59:10","doi":"10.21203/rs.3.rs-945016/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"74169c08-1488-4fa4-baa9-95248faf2b93","owner":[],"postedDate":"November 1st, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":8238964,"name":"Agroecology"},{"id":8238965,"name":"Evolutionary Biology"},{"id":8238966,"name":"Plant Molecular Biology and Genetics"}],"tags":[],"updatedAt":"2021-11-12T10:59:03+00:00","versionOfRecord":[],"versionCreatedAt":"2021-11-01 15:59:10","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-945016","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-945016","identity":"rs-945016","version":["v1"]},"buildId":"FbvkV6FR0MCFSLy54lSbu","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}