Syntopic call diversity among morphologically similar calling morphs of the paleotropical katydid of genusMecopoda(Tettigoniidae) from an Indo-Burma biodiversity hotspot

The acoustically diverse family of Tettigoniidae (Ensifera, Orthoptera) (commonly called bushcrickets or katydids), constitutes an excellent system to study the evolution of calling songs. Mecopoda is a genus that is particularly interesting because of the many morphologically cryptic calling morphs that have been described across South, East, and Southeast Asia, some of which are considered endemic to India. We describe five new syntopic and sympatric calling morphs of the genus Mecopoda that are morphologically similar but acoustically diverse from a sub-tropical forest in Meghalaya, in the Indo-Burma biodiversity hotspot. We report the morphological and acoustic characterization of these five call types, finding morphological overlap but well-separated acoustic clusters based on a PCA analysis of call temporal and spectral features. We also sequenced the COI gene mitochondrial gene of these five call types as well as other call types previously reported from India and performed a phylogenetic analysis of this gene relative to previously reported COI gene sequences from genus Mecopoda. Phylogenetic results suggest our five call types are not mutually monophyletic and relate from different subspecies of Mecopoda , of which three call types are genetically well separated from other calling morphs. This broadens our understanding of the evolution of acoustic diversity among paleotropical katydids.