Inactivation of Aspergillus niger conidia applying antifungal photocatalytic paints: a kinetic study

preprint OA: closed CC-BY-4.0
📄 Open PDF Full text JSON View at publisher

Abstract

Abstract Airborne fungal contamination in indoor environments is a growing concern, highlighting the need for innovative microbial control strategies. Developing novel photocatalytic materials for fungal control requires understanding the mechanisms and kinetics of photocatalytic inactivation. This study investigates the inactivation kinetics of Aspergillus niger conidia using a batch reactor and three TiO₂-based paints: rutile (non-photocatalytic), anatase (UV-active), and carbon-doped anatase (UV/Vis-active). Experiments were conducted at two relative humidity (RH) levels (50% and 70–80%) and three radiation fluxes (100%, 56%, and 18%). An empirical kinetic model was developed to describe the dependence of inactivation on radiation flux (power function) and RH (logistic model). Results showed a photocatalytic effect under UV light for photocatalytic paints, while photochemical effects dominated with non-photocatalytic paint. At 50% RH, logarithmic reductions per treatment day were 0.77 and 0.54 for anatase and rutile paints, respectively. At 70–80% RH, photocatalytic inactivation increased significantly, whereas rutile paint remained unchanged. The proposed kinetic model effectively captured the influence of radiation flux and RH, showing strong correlation with experimental data.
Full text 11,270 characters · extracted from preprint-html · click to expand
Inactivation of Aspergillus niger conidia applying antifungal photocatalytic paints: a kinetic study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Inactivation of Aspergillus niger conidia applying antifungal photocatalytic paints: a kinetic study Silvia Mercedes Zacarias, Delfina Martin, Valentina Wandel Petersen, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6298369/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Airborne fungal contamination in indoor environments is a growing concern, highlighting the need for innovative microbial control strategies. Developing novel photocatalytic materials for fungal control requires understanding the mechanisms and kinetics of photocatalytic inactivation. This study investigates the inactivation kinetics of Aspergillus niger conidia using a batch reactor and three TiO₂-based paints: rutile (non-photocatalytic), anatase (UV-active), and carbon-doped anatase (UV/Vis-active). Experiments were conducted at two relative humidity (RH) levels (50% and 70–80%) and three radiation fluxes (100%, 56%, and 18%). An empirical kinetic model was developed to describe the dependence of inactivation on radiation flux (power function) and RH (logistic model). Results showed a photocatalytic effect under UV light for photocatalytic paints, while photochemical effects dominated with non-photocatalytic paint. At 50% RH, logarithmic reductions per treatment day were 0.77 and 0.54 for anatase and rutile paints, respectively. At 70–80% RH, photocatalytic inactivation increased significantly, whereas rutile paint remained unchanged. The proposed kinetic model effectively captured the influence of radiation flux and RH, showing strong correlation with experimental data. airborne fungi decontamination air disinfection microorganisms photocatalytic paints titanium dioxide Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6298369","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":470016072,"identity":"c4c16b21-5c85-4200-8497-ffa4bf34cc0b","order_by":0,"name":"Silvia Mercedes Zacarias","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABKklEQVRIie3RsUrEMBjA8YRAbqnXNaFin0CwFKLCaV8lpdDpNqE4aafeUvfrdK9QF6cbAoXrUm6OOOgtnW7oIcgdnGgEvUFLudEh/ymB/AhfAoBO92/DuxUBoBd/r8XexPg5uif5UrybHI/uFk0TDTxgFotmMz2z7WzJXlZTcNSXHL5e/yWsKl06nod+DGYuTWvi5E/DU2dcA5dKjmjVQmSI0UFScABjpsYgMLeGzFLz+LnkgMYt5LnG6D358ADqvcGtIN4kq5i1FeBWEbRpIxJjBBMBY2wwpG7xY2kwS43OTyTHrbdUIaLpPPATw7iyDgUJ8iqMaCqIk1WL5LyNlDPYrKNLzzTL+9VS3FxMRsUDWYuB3S+D4rGF7MK/9upP1Yt0AJ1Op9N19AnefGYcMiXCtwAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0003-2351-3979","institution":"Instituto de Investigacion y Desarrollo en Bioingenieria y Bioinformatica","correspondingAuthor":true,"prefix":"","firstName":"Silvia","middleName":"Mercedes","lastName":"Zacarias","suffix":""},{"id":470016073,"identity":"c4834f0e-19e7-4d6d-812e-fb38a494fecc","order_by":1,"name":"Delfina Martin","email":"","orcid":"","institution":"Laboratorio I+D+i Microvidas","correspondingAuthor":false,"prefix":"","firstName":"Delfina","middleName":"","lastName":"Martin","suffix":""},{"id":470016074,"identity":"3c86907c-ecd2-4f42-af01-e96a0d9d07ae","order_by":2,"name":"Valentina Wandel Petersen","email":"","orcid":"","institution":"Centro Biotecnológico del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral","correspondingAuthor":false,"prefix":"","firstName":"Valentina","middleName":"Wandel","lastName":"Petersen","suffix":""},{"id":470016075,"identity":"db461e9e-32fe-49e8-9d69-66ad86f8b126","order_by":3,"name":"Orlando Mario Alfano","email":"","orcid":"","institution":"INTEC: Instituto de Desarrollo Tecnologico para la Industria Quimica","correspondingAuthor":false,"prefix":"","firstName":"Orlando","middleName":"Mario","lastName":"Alfano","suffix":""},{"id":470016076,"identity":"9c9fcbd6-b0ba-48dd-ad3a-2c490bc57ae1","order_by":4,"name":"Maria de los Milagros Ballari","email":"","orcid":"","institution":"INTEC: Instituto de Desarrollo Tecnologico para la Industria Quimica","correspondingAuthor":false,"prefix":"","firstName":"Maria","middleName":"de los Milagros","lastName":"Ballari","suffix":""}],"badges":[],"createdAt":"2025-03-24 21:26:24","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6298369/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6298369/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":84835456,"identity":"500e6817-4825-47b3-8424-08717d0f34a7","added_by":"auto","created_at":"2025-06-17 21:38:55","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":614408,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptZacariasetal.07042025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6298369/v1_covered_c5716080-9eac-473f-a00f-2c51924cc492.pdf"}],"financialInterests":"","formattedTitle":"Inactivation of Aspergillus niger conidia applying antifungal photocatalytic paints: a kinetic study","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"airborne, fungi, decontamination, air disinfection, microorganisms, photocatalytic paints, titanium dioxide","lastPublishedDoi":"10.21203/rs.3.rs-6298369/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6298369/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eAirborne fungal contamination in indoor environments is a growing concern, highlighting the need for innovative microbial control strategies. Developing novel photocatalytic materials for fungal control requires understanding the mechanisms and kinetics of photocatalytic inactivation. This study investigates the inactivation kinetics of Aspergillus niger conidia using a batch reactor and three TiO₂-based paints: rutile (non-photocatalytic), anatase (UV-active), and carbon-doped anatase (UV/Vis-active). Experiments were conducted at two relative humidity (RH) levels (50% and 70\u0026ndash;80%) and three radiation fluxes (100%, 56%, and 18%). An empirical kinetic model was developed to describe the dependence of inactivation on radiation flux (power function) and RH (logistic model). Results showed a photocatalytic effect under UV light for photocatalytic paints, while photochemical effects dominated with non-photocatalytic paint. At 50% RH, logarithmic reductions per treatment day were 0.77 and 0.54 for anatase and rutile paints, respectively. At 70\u0026ndash;80% RH, photocatalytic inactivation increased significantly, whereas rutile paint remained unchanged. The proposed kinetic model effectively captured the influence of radiation flux and RH, showing strong correlation with experimental data.\u003c/p\u003e","manuscriptTitle":"Inactivation of Aspergillus niger conidia applying antifungal photocatalytic paints: a kinetic study","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-16 05:34:38","doi":"10.21203/rs.3.rs-6298369/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"29472f48-119e-4fbc-8a55-aba445e06e74","owner":[],"postedDate":"June 16th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-06-17T21:30:47+00:00","versionOfRecord":[],"versionCreatedAt":"2025-06-16 05:34:38","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6298369","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6298369","identity":"rs-6298369","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

Ask this paper AI returns verbatim quotes from the full text · source: preprint-html

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. This is a recent paper (2025) — citers typically take a year or two to land, and the OpenAlex reference graph may still be filling in.

Source provenance

europepmc
last seen: 2026-05-20T01:45:00.602351+00:00
unpaywall
last seen: 2026-06-02T02:00:03.124865+00:00
License: CC-BY-4.0