Proliferation of endometrial stromal cells in endometriosis induced by Interleukin-1beta

Objective: To investigate the proliferation of endometrial stromal cells in endometriosis by interleukin-1beta(IL-1β).Methods: The eurotopic endometrial stromal cells of endometriosis cultured within 3～5 passages were treated with IL-1β in a dosage of 0 to 10.0?ng/ml,and then the cell proliferation and the cell cycle were measured by MTT and flow cytometry(FCM) respectively.Results: The endometrial stromal cells had been growing luxuriantly,and G_0/G_1 phase cells were few than G_2/M and S phase cells.Moreover,the proliferation was the most prominent when the cells were incubated in the presence of IL-1β at 1.0?ng/ml concentration for 12 hours.Conclusions: IL-1β induces the proliferation of endometrial stromal cells of endometriosis and promotes the transformation of G_0/G_1 phase cells into G_2/M and S phase.