Cell cycle-dependent transitions in PCP protein mobility erase and restore planar cell polarity in mitosis

ABSTRACT Central to our understanding of how cells establish polarity is deciphering how polarity proteins are transported to and retained at asymmetric positions. Here, in the context of planar cell polarity (PCP) remodeling in mitosis where asymmetry is transiently lost and restored, we show that PCP proteins transition between mobile and stable states in coordination with cell cycle progression. Live imaging and FRAP analyses reveal that, in addition to becoming internalized into endocytic vesicles, membrane-associated PCP proteins dramatically increase their lateral mobility upon mitotic entry in a Plk1-dependent manner. Unexpectedly, asymmetry is not restored in cytokinesis via polarized delivery of PCP-protein containing vesicles. Rather, PCP proteins continue to diffuse laterally during cytokinesis where they are captured and stabilized non-cell autonomously through adhesive interactions with neighbors. We propose that a transient, laterally diffusive state in mitosis allows for flexibility and robustness in planar polarity as dividing cells change shape and rearrange. Competing Interest Statement The authors have declared no competing interest. Footnotes Author list has been updated such they are now listed in the correct order.